Mucoid <i>Helicobacter pylori</i> Isolates with Fast Growth under Microaerobic and Aerobic Conditions

Siavoshi, Farideh; Saniee, Parastoo; Atabakhsh, Mojgan; Pedramnia, Shahrzad; Tavakolian, Atefeh; Mirzaei, Mehran

doi:10.1111/j.1523-5378.2011.00913.x

Cited by 6 publications

(4 citation statements)

References 39 publications

(49 reference statements)

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“…43 Furthermore, MC exhibited resistance to all the used antibiotics. These findings are in accordance with several reports that showed production of EXPs by H pylori could protect the bacterium against oxygen, antibiotics, 45 and stomach acid. 26 It appears that EXPs in Gram-negative bacteria protect against increased oxygen, 46 limitation of nitrogen and cations, 47 desiccation, 48 and antimicrobials.…”

Section: Discussionsupporting

confidence: 93%

Mucoid and coccoid Helicobacter pylori with fast growth and antibiotic resistance

2019

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Background In this study, one Helicobacter pylori isolate, from gastric biopsy of a dyspeptic patient that turned into mucoid‐coccoid (MC) form upon consecutive subcultures, was identified. The culturability, antibiotic resistance, and lipid contents of MC were compared with those of non‐mucoid (NM) spiral H pylori. Materials and Methods Mucoid‐coccoid and NM H pylori were subcultured on Brucella blood agar (BBA) and incubated under aerobic and microaerobic atmospheres at 37°C. Cultures were examined for colony characteristics and bacterial morphology after 1‐3 days. The isolates were identified by biochemical tests and detection of H pylori‐16S rDNA. Antibiogram was performed with currently used antibiotics for H pylori eradication. Cellular lipid contents were extracted and analyzed by gas chromatography. Results Compared with pin‐pointed and glistening colonies of NM H pylori that appeared under microaerobic conditions, MC H pylori grew well in consecutive subcultures under aerobic and microaerobic atmospheres and produced white patches of mucoid colonies. MC exhibited coccoid and NM spiral morphology. Both isolates were catalase, oxidase, and urease positive and contained 16S rDNA. Compared with NM that was susceptible to almost all the antibiotics, MC was resistant to all the antibiotics. Lipid analyses showed high frequency of unsaturated fatty acids and cholesterol in MC. Conclusions Coccoid forms with high fatty acid and cholesterol contents that show resistance to antibiotics might resist against other stressful conditions such as gastric acidity and immune response. Moreover, mucoid property may enhance resistance of coccoids to stresses. With mucoid‐coccoid lifestyle, H pylori may establish a chronic infection refractory to antimicrobial therapy.

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Section: Discussionsupporting

confidence: 93%

Mucoid and coccoid Helicobacter pylori with fast growth and antibiotic resistance

2019

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“…In this study, we substantiate that the amount of carbohydrates composing the EPS matrix seems to differ according to the medium used to form the biofilm. Furthermore, it has been hypothesized that the in vivo production of EPS by H. pylori may protect the bacterium against stomach acid and pH fluctuations and may serve as a physical barrier to reduce the uptake of antibiotics .…”

Section: Discussionmentioning

confidence: 99%

Helicobacter pylori free‐living and biofilm modes of growth: behavior in response to different culture media

Bessa

Grande

Iorio

et al. 2012

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The physiological behavior of Helicobacter pylori in different growth conditions was investigated to approach its growth standardization. H. pylori free-living and biofilm modes of growth were assessed in four different liquid culture media (Brucella broth, brain heart infusion broth and Ham's F-12 supplemented with 2% fetal calf serum and Ham's F-12 without serum). Free-living growth was monitored during 72 h in each medium and characterized for bacterial density, culturability, viability and morphology. The biofilm formation in the same media was evaluated for biomass production, colony forming unit (CFU) counts and microscopic visualization. Afterward, using Ham's F-12, the effect of amoxicillin and clarithromycin at sub- minimum inhibitory concentrations (sub-MICs) was evaluated on H. pylori biofilm formation and luxS gene expression. Differences in the free-living growth were observed between the media supplemented with serum and Ham's F-12 without serum. Biofilm formation was significantly dependent on the growth media used. Ham's F-12 seems to be a good medium to support both growth phenotypes of H. pylori. Moreover, sub-MICs of antibiotics increased the biofilm formation and affected the luxS gene expression. Optimizing the growth conditions of H. pylori, especially in the biofilm mode, will be helpful to perform more accurate in-depth studies that will allow increasing the knowledge about H. pylori biofilm, which should be a target to eradicate resistant infection.

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“…Cell surface hydrophobicity plays a crucial function in resistance, pathogen-host interactions, and biofilm architecture. Production of exopolysaccharide, active clustering, and the cells embedded in an extracellular matrix conquers the impact of stomach acid and pH fluctuations and increases resistance to antibiotics [11,44,45]. AiiA-AgNPs reduces the hydrophobicity index of H. pylori, which leads to the inhibition of biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Influence of N‐acylhomoserine lactonase silver nanoparticles on the quorum sensing system of Helicobacter pylori: A potential strategy to combat biofilm formation

et al. 2020

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The treatment of Helicobacter pylori usually fails due to their ability to form biofilms and resistance to antibiotics. This might potentially lead to gastric carcinoma and mucosa‐associated lymphoid tissue lymphoma. In the present study, we elucidate the potential role of N‐acylhomoserine lactonase stabilized silver nanoparticles (AiiA‐AgNPs) in treating biofilms produced by H. pylori. AiiA‐AgNPs inhibited quorum sensing (QS) by degradation of QS molecules, thereby reducing biofilm formation, urease production, and altering cell surface hydrophobicity of H. pylori. AiiA‐AgNPs showed no cytotoxic effects on RAW 264.7 macrophages at the effective concentration (1–5 µM) of antibiofilm activity. In addition, AiiA‐AgNP in high concentration (80–100 µM) exhibited cytotoxicity against HCT‐15 carcinoma cells, depicting its therapeutic role in treating cancer.

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Mucoid Helicobacter pylori Isolates with Fast Growth under Microaerobic and Aerobic Conditions

Cited by 6 publications

References 39 publications

Mucoid and coccoid Helicobacter pylori with fast growth and antibiotic resistance

Mucoid and coccoid Helicobacter pylori with fast growth and antibiotic resistance

Helicobacter pylori free‐living and biofilm modes of growth: behavior in response to different culture media

Influence of N‐acylhomoserine lactonase silver nanoparticles on the quorum sensing system of Helicobacter pylori: A potential strategy to combat biofilm formation

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