Mucoadhesive microparticulate drug delivery system of curcumin against Helicobacter pylori infection: Design, development and optimization

Ali, Mushir; Pandit, Vinay; Jain, Mahendra; Dhar, K.L.

doi:10.4103/2231-4040.126996

Cited by 33 publications

(16 citation statements)

References 16 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Curcumin is a natural active ingredient extracted from dry rhizome of the turmeric Curcuma genus plant, turmeric and Curcuma, with extensive pharmacological effects, low toxicity, and good tolerance, and due to its economic value, it has become a hot spot for exploration (8). Findings of studies focusing on curcumin have identified a wide range of pharmacological activities such as anti-inflammation, anti-oxidation, lipid, anti-virus, anti-infection, anticancer, anticoagulant, anti-liver fibrosis and atherosclerosis, low toxicity and few side effects (9)(10)(11)(12)(13). However, the effect of curcumin on NSCLC and the underlying molecular mechanism remains unclear.…”

Section: Curcumin Inhibits Cell Proliferation and Induces Apoptosis Omentioning

confidence: 99%

Curcumin inhibits cell proliferation and induces apoptosis of human non-small cell lung cancer cells through the upregulation of miR-192-5p and suppression of PI3K/Akt signaling pathway

et al. 2015

View full text Add to dashboard Cite

Curcumin is the main active ingredient extracted from the traditional Chinese medicine, turmeric, which acts against non-small cell lung cancer cell (NSCLC), lowers blood pressure, is anti-inflammatory, choleretic, and exerts anti‑oxidant effects, without any obvious toxicity in the long term. The aim of the present study was to investigate whether the anticancer effect of curcumin inhibited cell proliferation and induced apoptosis of human NSCLC through the upregulation of microRNA‑192-5p (miR-192-5p) and suppression of the PI3K/Akt signaling pathway. In the present study, treatment with curcumin inhibited cell proliferation, induced cell apoptosis and increased the caspase-3 activity of A549 cells. The results also showed that, miR-192-5p relative expression of NCL-H460 cells was relatively lower than that of A549 cells, which was higher, with that of BEAS-2E cells being the highest. miR-192-5p mimics suppressed cell proliferation and increased cell apoptosis of A549 cells. However, anti-miR-192-5p mimics increased cell proliferation and inhibited cell apoptosis of A549 cells. Curcumin treatment effectively increased the relative miR‑192-5p expression and suppressed the PI3K/Akt signaling pathway. miR-192-5p mimics enhanced the effect of curcumin on cell viability and apoptosis and suppressed the PI3K/Akt signaling pathway in A549 cells. Anti-miR-192-5p mimics reversed the effect of curcumin on A549 cells and PI3K/Akt expression. Collectively, our findings suggested that curcumin inhibited cell proliferation and induced apoptosis of human non-small cell lung cancer cells through the upregulation of miR-192-5p and suppression of the PI3K/Akt signaling pathway.

show abstract

Section: Curcumin Inhibits Cell Proliferation and Induces Apoptosis Omentioning

confidence: 99%

Curcumin inhibits cell proliferation and induces apoptosis of human non-small cell lung cancer cells through the upregulation of miR-192-5p and suppression of PI3K/Akt signaling pathway

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Studies are available pertaining to the determination of minimum inhibitory concentration (MIC) values of curcumin apart from documentation of its anti-quorum sensing and anti-efflux pump activities ( Bhawana et al, 2011 ; Mun et al, 2013 ; Ali et al, 2014 ; Packiavathy et al, 2014 ; Zorofchian Moghadamtousi et al, 2014 ; Pandit et al, 2015 ; Tyagi et al, 2015 ). While determining the MIC of curcumin against different bacterial isolates we faced difficulty in determining and interpreting the results possibly due to its insolubility in aqueous phase leading to formation of colored precipitate.…”

Section: Introductionmentioning

confidence: 99%

Curcumin Quantum Dots Mediated Degradation of Bacterial Biofilms

et al. 2017

View full text Add to dashboard Cite

Bacterial biofilm has been reported to be associated with more than 80% of bacterial infections. Curcumin, a hydrophobic polyphenol compound, has anti-quorum sensing activity apart from having antimicrobial action. However, its use is limited by its poor aqueous solubility and rapid degradation. In this study, we attempted to prepare quantum dots of the drug curcumin in order to achieve enhanced solubility and stability and investigated for its antimicrobial and antibiofilm activity. We utilized a newer two-step bottom up wet milling approach to prepare Curcumin Quantum Dots (CurQDs) using acetone as a primary solvent. Minimum inhibitory concentration against select Gram-positive and Gram-negative bacteria was performed. The antibiofilm assay was performed at first using 96-well tissue culture plate and subsequently validated by Confocal Laser Scanning Microscopy. Further, biofilm matrix protein was isolated using formaldehyde sludge and TCA/Acetone precipitation method. Protein extracted was incubated with varying concentration of CurQDs for 4 h and was subjected to SDS–PAGE. Molecular docking study was performed to observe interaction between curcumin and phenol soluble modulins as well as curli proteins. The biophysical evidences obtained from TEM, SEM, UV-VIS, fluorescence, Raman spectroscopy, and zeta potential analysis confirmed the formation of curcumin quantum dots with increased stability and solubility. The MICs of curcumin quantum dots, as observed against both select gram positive and negative bacterial isolates, was observed to be significantly lower than native curcumin particles. On TCP assay, Curcumin observed to be having antibiofilm as well as biofilm degrading activity. Results of SDS–PAGE and molecular docking have shown interaction between biofilm matrix proteins and curcumin. The results indicate that aqueous solubility and stability of Curcumin can be achieved by preparing its quantum dots. The study also demonstrates that by sizing down the particle size has not only enhanced its antimicrobial properties but it has also shown its antibiofilm activities. Further, study is needed to elucidate the exact nature of interaction between curcumin and biofilm matrix proteins.

show abstract

“…Results revealed the permanency of microparticles in the deepest layers of the mucosa for over 6 h [29]. [48,49] Proteins Gliadin Load acetohydroxamic acid [50]; amoxicillin [51]; clarithromycin [52]; dual therapy [53] and triple therapy [54] Gelatin Load amoxicillin [55,56] Chitosan Deliver dual therapy [57,58]; amoxicillin [17,59]; tetracycline [44]; Antibacterial properties of chitosan [46,60,61] Copolymers Deliver antibiotics (several systems with different mixture of polymers, summarised in [22]); probiotics [62]; phytomedicine [63][64][65][66] and antacids [67].…”

Section: Mucoadhesivenessmentioning

confidence: 99%