Mucins, Mucus, and Sputum

Voynow, Judith A.; Rubin, Bruce K.

doi:10.1378/chest.08-0412

Cited by 456 publications

(394 citation statements)

References 77 publications

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“…Mucins constitute an important element of natural immunity and its secretion is associated with different physiological and pathological conditions (Voynow et al ., 2009;Nakamura et al ., 2012). Mucins are classified as neutral, acidic, sulphated, and non-sulphated.…”

Section: Goblet Cells Synthesize Glycoproteins Called Mucinsmentioning

confidence: 99%

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

Zapata

Rodríguez²,

Ramírez³

et al. 2015

Rev Colomb Cienc Pecu

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El lipopolisacárido de Escherichia coli afecta la secreción de mucinas intestinales en cerdos destetados O lipopolissacárido de Escherichia coli afecta a secreção de mucina intestinal em porcos desmamadosSummary Background: to the best of our knowledge, the effects of lipopolysaccharide (LPS) from Escherichia coli on goblet cells and intestinal mucin secretion of weaned pigs has not been reported, and it is unknown whether these effects could trigger enteritis. Objective: to determine the effect of E . coli LPS on intestinal mucin secretion in weaning piglets. Methods: fifty-two piglets weaned at 21 days of age were fed a basal diet supplemented with four LPS levels (0.0, 0.3, 0.5, and 1.0 µg/mg) during 10 days. Piglets were slaughtered on days 1, 5, 7, and 10 post-weaning and samples of small and large intestine were taken for histochemical staining to determine goblet cell population and type of mucins produced (acidic, sulphated, non-sulphated, or neutral). Results: acidic mucin was reduced on day 5 post-weaning independently of the dietary LPS level supplied to piglets. Recovery of acidic mucins was observed during days 7 and 10 post-weaning. Neutral mucins increased on day 5 and decreased on days 7 and 10 post-weaning. High LPS levels decreased goblet cells population and secretion of all types of mucins. This effect was remarkably high for diet two (D2: 0.5 mg LPS/mg food). Conclusions: early weaning (21 d) and LPS addition to the diet affect mucin secretion and intestinal epithelium integrity by modifying goblet cell populations and their balance between acidic and neutral mucin secretion. These findings explain some abnormalities related with post-weaning diarrhea syndrome and help to explain its pathophysiology.

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Section: Goblet Cells Synthesize Glycoproteins Called Mucinsmentioning

confidence: 99%

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

Zapata

Rodríguez²,

Ramírez³

et al. 2015

Rev Colomb Cienc Pecu

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“…Su principal componente macromolecular son las mucinas, una familia de glicoproteínas de elevado peso molecular (2 a 20 x 10 5 Da), altamente glicosiladas (50 a 90% de su masa son carbohidratos). Estas glicoproteínas son estructuralmente variadas, secretadas de manera constitutiva por las células caliciformes, y también pueden ser secretadas como respuesta a un estí-mulo agudo, lo que ocurre en milisegundos 4,6,8 . Las mucinas dotan al moco de características viscoelásticas que le permiten actuar como gel y capturar partículas inhaladas, sus cargas eléctrica-mente negativas generan fuerzas de repulsión contra bacterias y otros patógenos [10][11][12] .…”

Section: Capa Mucosaunclassified

“…La primera línea de defensa es el barrido mucociliar del epitelio respiratorio que, mediante la secreción constitutiva de moco mantiene la hidratación de la vía aérea, atrapa partículas, bacterias y virus, y contribuye sustancias antioxidantes, antiproteasas y antimicrobianas. Este barrido mucociliar elimina el moco en un proceso que exige una regulación muy fina entre el volumen y composición del moco y el volumen de líquido periciliar, de tal forma que permitan un adecuado batido ciliar 5,6 . Numerosos factores pueden alterar este balance al inducir sobreproducción e hipersecreción de moco o activar respuestas como la producción de sustancias antivirales, antibióticas y citoquinas proinflamatorias 7 .…”

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Dinámica y patología del barrido mucociliar como mecanismo defensivo del pulmón y alternativas farmacológicas de tratamiento

R³

et al. 2014

Rev. méd. Chile

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The first line of defense to maintain the sterility of the intrapulmonaryA l menos 12.000 litros de aire son filtrados por los pulmones de un ser humano en un día 1 . Este enorme volumen contiene el oxígeno necesario para las funciones vitales, alérgenos, microorganismos, partículas y otros gases 2 . Al igual que otros epitelios, el respiratorio está protegido por defensas que incluyen barreras anatómicas, cambios aerodinámicos, secreción de moco, tos, macrófagos alveolares y moléculas con efecto antibiótico 3,4 . La primera línea de defensa es el barrido mucociliar del epitelio respiratorio que, mediante la secreción constitutiva de moco mantiene la hidratación de la vía aérea, atrapa partículas, bacterias y virus, y contribuye sustancias antioxidantes, antiproteasas y antimicrobianas. Este barrido mucociliar elimina el moco en un proceso que exige una regulación muy fina entre el volumen y composición del moco y el volumen de líquido periciliar, de tal forma que permitan un adecuado batido ciliar 5,6 . Numerosos factores pueden alterar este balance al inducir sobreproducción e hipersecreción de moco o activar respuestas como la producción de sustancias antivirales, antibióticas y citoquinas proinflamatorias 7 . Estas respuestas pueden contribuir a la patogénesis de las enfermedades respiratorias comunes 8 .Esta revisión describe la composición y funcionamiento del barrido mucociliar, sus alteraciones y terapias disponibles. Barrido mucociliarEl barrido mucociliar requiere de equilibrio y coordinación entre sus tres componentes: la capa mucosa superficial en contacto con el lumen, capaz de adherir virtualmente cualquier partícula

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“…Mucins are multimillion dalton glycoproteins which are present in the airway mucus and produced by goblet cells in the surface epithelium and mucous cells in the submucosal gland (Rogers and Barnes, 2006). Hypersecretion of airway mucus, however, is one of the major symptoms associated with severe pulmonary diseases including asthma, chronic bronchitis, cystic fi brosis and bronchiectasis (Voynow and Rubin, 2009). Therefore, we suggest it is valuable to determine the potential activities of compounds derived from various medicinal plants for inhibiting excess mucin production.…”

Section: Introductionmentioning

confidence: 99%

Effect of Berberine on MUC5AC Mucin Gene Expression and Mucin Production from Human Airway Epithelial Cells

Sikder¹,

Lee²,

Lee³

et al. 2011

Biomolecules and Therapeutics

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We conducted this study to investigate whether berberine signifi cantly affects MUC5AC mucin gene expression and mucin production induced by epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-α (TNF-α) from human airway epithelial cells. Confl uent NCI-H292 cells were pretreated with varying concentrations of berberine for 30 min and then stimulated with EGF, PMA or TNF-α for 24 h. MUC5AC mucin gene expression and mucin production were measured by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Berberine was found to inhibit the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-α. Berberine also inhibited the production of MUC5AC mucin protein stimulated by the same inducers. This result suggests that berberine can regulate the expression of mucin gene and production of mucin protein, by directly acting on human airway epithelial cells. Abstractber of reports, Coptis japonica Makino and its components, berberine, have been used to control airway allergic or infl ammatory diseases and reported to have anti-infl ammatory and anti-cancer effects (Yoo et al., 2008;Sun et al., 2009;Tang et al., 2009). On the other hand, it was previously reported that berberine stimulated basal mucin release from airway goblet cells, by our group . However, to the best of our knowledge, there are no reports about the effect of berberine on MUC5AC mucin gene expression and mucin production induced by epidermal growth factor (EGF), phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-α (TNF-α), from human airway epithelial cells. Therefore, in this study, we investigated whether berberine affects the expression of MUC5AC mucin gene and the production of mucin protein induced by EGF, PMA or TNF-α, from the NCI-H292 human pulmonary mucoepidermoid cell line.

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Mucins, Mucus, and Sputum

Cited by 456 publications

References 77 publications

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

Escherichia coli lipopolysaccharide affects intestinal mucin secretion in weaned pigs

Dinámica y patología del barrido mucociliar como mecanismo defensivo del pulmón y alternativas farmacológicas de tratamiento

Effect of Berberine on MUC5AC Mucin Gene Expression and Mucin Production from Human Airway Epithelial Cells

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