Mucin pre-cultivated Lactobacillus reuteri E shows enhanced adhesion and increases mucin expression in HT-29 cells

Dudík, Boris; Sepová, Hana Kiňová; Bilka, František; Pašková, Ľudmila; Bilková, Andrea

doi:10.1007/s10482-020-01426-1

Cited by 16 publications

(22 citation statements)

References 46 publications

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“…It has been reported that the probiotics such as Bifidobacterium and Lactobacillus could promote the secretion of mucins (Round et al, 2012;Wrzosek et al, 2013;Nishiyama et al, 2015;Arike et al, 2017). C. butyricum is an important commensal bacterium inhabiting human gut, which has been used as a clinical drug to cure intestinal inflammation in Japan (Dudik et al, 2020). In the present study, the fluorescence microscopy analysis revealed that co-culture with C. butyricum resulted in a marked elevation of the mucin secretion and/or glycosylation of the mucus in HT-29 cells.…”

Section: Discussionsupporting

confidence: 49%

“…One limitation of the study is that it was performed in HT-29 cell line, which was a cancer cell line, and not normal human tissue, but studies have shown that HT-29 cell line is able to produce and secrete mucins and form a mucus layer, which could be used as in vitro intestinal epithelium model. (Gagnon et al, 2013;Dudik et al, 2020). In this work, we explored the adhesion capability of C. butyricum to intestinal luminal epithelium by using HT-29 cells as an in vitro model.…”

Section: Discussionmentioning

confidence: 99%

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Clostridium butyricum Induces the Production and Glycosylation of Mucins in HT-29 Cells

Lu²,

Mao

et al. 2021

Front. Cell. Infect. Microbiol.

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C. butyricum is a common gut commensal bacterium, which has many positive functions in human intestine. In this study, we investigated the effects of monosaccharide and its derivatives on the adhesion of C. butyricum to the mucus of HT-29 cells. RNA interference was performed to assess the roles of MUC2 and glycan in the adhesion of C. butyricum to HT-29 cells. The effects of C. butyricum on the glycosylation of mucins were assayed with fluorescence microscope. The expression levels of mucins and glycotransferases were also determined. The results showed that C. butyricum could adhere to the mucins secreted by HT-29 cells. Several kinds of monosaccharides inhibited the adhesion of C. butyricum to HT-29 cells, which suggested that the mucus glycan was the attaching sites of this bacterium. Knockdown of MUC2, FUT2 or GALNT7 significantly decreased the numbers of the bacteria adhering to HT-29 cells. When colonizing on the surface of HT-29 cells, C. butyricum could increase the production of mucins, promote the expression of glycotransferase, and induce the glycosylation of mucins. These results demonstrated that the glycan of mucus played important roles in the adhesion of C. butyricum to HT-29 cells. This study indicates for the first time that C. butyricum possesses the ability to modulate the glycosylation profile of mucus secreted by HT-29 cells. These findings contribute to understanding the mechanism of interaction between colonic epithelial cells and commensal bacteria.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Clostridium butyricum Induces the Production and Glycosylation of Mucins in HT-29 Cells

Lu²,

Mao

et al. 2021

Front. Cell. Infect. Microbiol.

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“…In the gastrointestinal tract, commensal lactobacilli have genes encoding mucin binding protein or other binding factors allowing adhesion to mucins. Similar genomic features are noted in L. crispatus and L. gasseri, two of the most common bacteria of the vaginal microbiota (Boekhorst et al, 2006;Velez et al, 2007;Van Tassell and Miller, 2011;Dudik et al, 2020). The ability to use CVM may facilitate Lactobacillus dominance of the vaginal microbiome.…”

Section: Impact Of Cervicovaginal Mucus On Genital Microbiotamentioning

confidence: 59%

“…Few studies examine the role of mucins in driving FGT microbial communities. Changes in mucin composition due to hormones, or disruption of CVM by pathogen mucinase activity could limit Lactobacillus binding, and “non-optimal” microbial populations could prevail ( Dudik et al., 2020 ). In the gut, different mucin glycosylation patterns are instrumental to microbial tropism ( Tailford et al., 2015 ).…”

Section: Impact Of Cervicovaginal Mucus On Genital Microbiotamentioning

confidence: 99%

“…These same mucins downregulate virulence genes in the respiratory pathogen P. aeruginosa and decrease biofilm formation ( Wheeler et al., 2019 ). Pre-incubation of L. reuteri (a common gut species) with gastric mucin led to greater adherence to HT-29 colon epithelial cells, and upregulation of bacterial surface adhesion proteins ( Dudik et al., 2020 ). Apart from C. albicans , few genital tract microbes have been evaluated in these types of experiments.…”

Section: Impact Of Cervicovaginal Mucus On Genital Microbiotamentioning

confidence: 99%

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Mutual Preservation: A Review of Interactions Between Cervicovaginal Mucus and Microbiota

Vagios

Mitchell

2021

Front. Cell. Infect. Microbiol.

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At mucosal surfaces throughout the body mucus and mucins regulate interactions between epithelia and both commensal and pathogenic bacteria. Although the microbes in the female genital tract have been linked to multiple reproductive health outcomes, the role of cervicovaginal mucus in regulating genital tract microbes is largely unexplored. Mucus-microbe interactions could support the predominance of specific bacterial species and, conversely, commensal bacteria can influence mucus properties and its influence on reproductive health. Herein, we discuss the current evidence for both synergistic and antagonistic interactions between cervicovaginal mucus and the female genital tract microbiome, and how an improved understanding of these relationships could significantly improve women’s health.

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Inhibitory activity of Limosilactobacillus reuteri isolated from camel milk against Helicobacter pylori effects in human gastric epithelial cells

Nia

Ghasemi

Saeidi

et al. 2023

Biotech and App Biochem

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This study aimed to evaluate anti‐Helicobacter pylori effects of Limosilactobacillus reuteri 2892 (L. reuteri 2892) isolated from camel milk in GC cell lines (AGS and MKN). From 15 camel milk samples, 132 microbial strains were isolated. Based on microbial and biochemical analysis, 11 potential probiotic candidates were selected. The potential probiotic candidates were assayed for anti‐H. pylori activity, and the strain with the highest anti‐H. pylori activity was identified genotypically. Based on 16S rDNA sequencing, the selected strain with the best activity against H. pylori (inhibition zone = 15.5 ± 0.8) belonged to the Lactobacillus reuteri strain 2892. Cell treatment with H. pylori HC‐113 inhibits gene expression of Claudin‐4, ZO‐1, MUC5AC, and MUC2 in gastric cells, which are attenuated by L. reuteri 2892. The simulative effects of H. pylori HC‐113 on the cell migration and invasion of gastric cells were lost when cells were cotreated with L. reuteri 2892. Cell treatment with H. pylori HC‐113 promoted cell death, whereas cotreatment with L. reuteri 2892 markedly decreased necrotic and late apoptotic cells. The present study demonstrates that L. reuteri 2892 has potent anti‐H. pylori effects and thus can be considered as an alternative protective agent against inflammatory effects of H. pylori in gastric cells.

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Mucin pre-cultivated Lactobacillus reuteri E shows enhanced adhesion and increases mucin expression in HT-29 cells

Cited by 16 publications

References 46 publications

Clostridium butyricum Induces the Production and Glycosylation of Mucins in HT-29 Cells

Clostridium butyricum Induces the Production and Glycosylation of Mucins in HT-29 Cells

Mutual Preservation: A Review of Interactions Between Cervicovaginal Mucus and Microbiota

Inhibitory activity of Limosilactobacillus reuteri isolated from camel milk against Helicobacter pylori effects in human gastric epithelial cells

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