MUC1 mucin mRNA expression in stage I lung adenocarcinoma and its association with early recurrence

Ohgami, Akira; Tsuda, Tohru; Osaki, Toshihiro; Mitsudomi, Tetsuya; Morimoto, Yasuo; Higashi, Toshiaki; Yasumoto, Kosei

doi:10.1016/s0003-4975(99)00041-7

Cited by 33 publications

(22 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both studies, including the more common administration of adjuvant chemotherapy and radiotherapy for more advanced disease, are likely to have introduced confounding factors affecting the application of molecular analysis to prognosis. Interestingly, Ohgami et al [28] reported that there was a strong link between MUC1 mRNA expression and early recurrence and hematogenic metastasis in patients with stage I lung adenocarcinoma which is fairly in agreement with the present study. MUC1 is a transmembrane mucin consisting of a heavily O-glycosylated extracellular domain, a transmembrane domain, and a cytoplasmic tail.…”

Section: Discussionsupporting

confidence: 93%

“…However, in the further stratified analysis split by histology, the prognostic significance only remained in the non-SCC subgroup. These findings are consistent with several previous studies [22,[28][29][30]. Nevertheless, Jarrard and his colleagues [23] reported that MUC1 expression did not imply more aggressive malignant behaviors of NSCLC or predict the poor prognosis of patients despite significant correlation with adenocarcinoma histology.…”

Section: Discussionsupporting

confidence: 93%

“…To date, numerous immunohistochemistry studies have been performed in order to elucidate the role of MUC1 in NSCLC; however, the current findings in terms of its expression pattern and potential involvement in the formation and progression of NSCLC are contradictory in the literatures [5,[21][22][23][27][28][29][30]. One problem faced by researchers is the determination of the extent of tumor immunohistochemical positivity for MUC1 which is clinically and biologically relevant.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Expression and prognostic relevance of MUC1 in stage IB non-small cell lung cancer

Situ

Wang

et al. 2010

Med Oncol

View full text Add to dashboard Cite

The goal of this study was to evaluate the expression of MUC1 in stage IB non-small cell lung cancer (NSCLC) and its prognostic significance. The expression of MUC1 in 178 NSCLC specimens was evaluated via immunohistochemistry. A reproducible semiquantitative method which took both staining percentage and intensity into account was applied for immunohistochemical scoring, and receiver operating characteristic curve analysis was utilized to select the cut-off score for high or low MUC1 expression. Then, the correlations between MUC1 expression and clinicopathological features and its prognostic relevance were determined. In this study, high MUC1 expression was detected more frequently in adenocarcinomas (86.3%) and other NSCLCs (74.1%) than in squamous cell carcinomas (39.1%, P < 0.001). The Kaplan-Meier survival curves showed that up-regulated expression of MUC1 indicated poorer overall survival (OS) and disease-free survival (DFS) (P = 0.011 and P = 0.008, respectively), especially for those with non-squamous cell carcinomas (P = 0.033 and P = 0.011, respectively). Multivariate analysis also confirmed that MUC1 expression was an independent prognostic factor for both OS and DFS in stage IB NSCLC (P = 0.008 and P = 0.004, respectively). MUC1 might be correlated with the histogenesis of lung adenocarcinoma, and its elevated expression may be an adverse prognostic indicator for the patients with stages IB NSCLC, particularly for those with non-squamous cell carcinomas.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression and prognostic relevance of MUC1 in stage IB non-small cell lung cancer

Situ

Wang

et al. 2010

Med Oncol

View full text Add to dashboard Cite

show abstract

“…The high expression level of MUC1 in lung cancer has been closely correlated with early recurrence, poor prognosis and high metastatic potential (19). Ren et al (20) suggested that downregulation of MUC1 in lung cancer A549 cells increased their sensitivity to genotoxic drugs in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

MUC1 downregulation inhibits non‑small cell lung cancer progression in human cell lines

Wang

et al. 2017

Exp Ther Med

View full text Add to dashboard Cite

Abstract. Mucin 1 (MUC1) is a transmembrane glycoprotein that is aberrantly unregulated in numerous types of cancer, including non-small cell lung cancer (NSCLC), and serves a key role as an oncogene in the tumorigenesis of various human adenocarcinomas. Studies have indicated that MUC1 is involved in cell proliferation, invasion and migration. However, the role of MUC1 in NSCLC progression remains poorly understood. The aim of the present study was to investigate the role of MUC1 in stable MUC1-low-expression NSCLC cell lines that were generated by transfection with MUC1-siRNA. Cell Counting Kit-8 assay was preformed to determine the proliferation ability of NSCLC cells, while cell apoptosis was detected using flow cytometry. In addition, the mRNA and protein expression levels of MUC1 were detected by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. Western blot analysis was also used for detection of other associated proteins. The results demonstrated that, compared with the control group, the cell proliferation ability was significantly declined in the MUC1 inhibition group, and the cell apoptosis rate was markedly increased. Inhibition of MUC1 gene in NCI-H1650 cells suppressed cell proliferation and induced cell apoptosis. In addition, the protein expression levels of vascular endothelial growth factor (VEGF) and VEGF-C were notably decreased by MUC1 inhibition, indicating the anti-angiogenic effect of MUC1 downregulation. Furthermore, inhibition of MUC1 gene with MUC1-siRNA significantly suppressed the phosphorylation of protein kinase B and extracellular signal-regulated kinase. In conclusion, the findings indicated that silencing of MUC1 gene may inhibit the development of NSCLC cells.

show abstract

“…In the past few years, O-linked carbohydrate antigens, such as mucin antigen (MUC1), carcinoembryonic antigen (CEA), sialyl LewisX (SLX), CA19-9, Sialyl Tn (STn), and Tn have been reported to be associated with altered adhesion (Fogel et al, 1983;Rice and Bevilacqua, 1989), invasion (Bolscher et al, 1988;Matsushita et al, 1991;Nakamori et al, 1994), recurrence, and prognosis (Itzkowitz et al, 1990;Niklinski et al, 1992;Ogawa et al, 1994;Cao et al, 1995;Diez et al, 1996;Ohgami et al, 1999) in lung cancer and other tumours. Mucin-type O-linked carbohydrates may constitute up to 80% of the total mass of these glycoproteins ; O-glycosylation has been shown to be important in the binding of cell adhesion molecules, cell differentiation, invasion, and metastasis in tumours (Nakamori et al, 1994;Clausen and Bennett, 1996;Sutherlin et al, 1997;Nomoto et al, 1999).…”

mentioning

confidence: 99%

Low expression of polypeptide GalNAc N-acetylgalactosaminyl transferase-3 in lung adenocarcinoma: impact on poor prognosis and early recurrence

Oyama

Osaki

et al. 2004

Br J Cancer

View full text Add to dashboard Cite

MUC1 mucin mRNA expression in stage I lung adenocarcinoma and its association with early recurrence

Cited by 33 publications

References 23 publications

Expression and prognostic relevance of MUC1 in stage IB non-small cell lung cancer

Expression and prognostic relevance of MUC1 in stage IB non-small cell lung cancer

MUC1 downregulation inhibits non‑small cell lung cancer progression in human cell lines

Low expression of polypeptide GalNAc N-acetylgalactosaminyl transferase-3 in lung adenocarcinoma: impact on poor prognosis and early recurrence

Contact Info

Product

Resources

About