MUC1 gene silencing inhibits proliferation, invasion, and migration while promoting apoptosis of oral squamous cell carcinoma cells

Abstract

The aim of the present study is to investigate the role of RNA interference in the inhibition of MUC1 gene expression in occurrence and metastasis of oral squamous cell carcinoma (OSCC) and its in-depth mechanisms. The OSCC and normal oral mucosa tissues, as well as normal oral epithelial cell line HOK and OSCC cell line SCC-4, Cal-27, TSCCA, Tca8113 were obtained to detect the expression of MUC1. Slug expression in OSCC and normal oral mucosa tissues was also determined. The OSCC cells were grouped to investigate the role of MUC1 gene silencing on proliferation, DNA replication, cell cycle distribution, apoptosis, colony formation ability, epithelial-mesenchymal transition (EMT), invasion, and migration of OSCC cells. We first found higher positive rate of MUC1 and Slug expression in OSCC tissues. Next, it was determined that higher expression of MUC1 was found in OSCC tissues and cells. Furthermore, silencing of MUC1 declined Slug expression, inhibited the proliferation, DNA replication, cell cycle progression, and EMT while inducing apoptosis of OSCC cells. Our study suggests that overexpression of MUC1 is found in OSCC, and MUC1 gene silencing could inhibit the proliferation, invasion, and migration while inducing apoptosis of OSCC cells.

Identification of Novel Prognostic Risk Signatures of Soft Tissue Sarcoma Based on Ferroptosis-Related Genes

“…Besides, mucin 1 (MUC1), a tumor driver gene in our study, is a membranebound protein whose gene expression is highest in the respiratory, digestive, and reproductive systems, and plays a role in cell growth, differentiation, and cell signal transduction (35)(36)(37). In oral squamous cell carcinoma (OSCC), silencing MUC1 can reduce the expression of Slug, thereby inhibiting tumor cell proliferation, inhibiting DNA replication, and inducing OSCC cell apoptosis (38). In addition, MUC1 can also be used as a useful marker for predicting poor prognostic factors for 5-year survival outcome after radical esophageal squamous cell carcinoma resection (39).…”

Baicalein Represses Cervical Cancer Cell Growth, Cell Cycle Progression and Promotes Apoptosis via Blocking AKT/mTOR Pathway by the Regulation of circHIAT1/miR-19a-3p Axis

“…28 Juan et al have shown that miR-489 served for an anti-proliferative and pro-apoptotic maker in CC cells, 29 and miR-148a was used as a novel target to suppress the progression of CC. 30 Interestingly, we found that miR-19a-3p was highly expressed in CC tissues and cells but its expression was reduced after baicalein treatment in CC cells, which demonstrated that the biological function of baicalein in CC might be related to the downregulation of miR-19a-3p. Our reverted assays subsequently exhibited that the overexpression of miR-19a-3p recovered the regulation of baicalein in CC cells, validating that baicalein functioned in CC cells via the expression inhibition of miR-19a-3p.…”

Changes in Protein Glycosylation in Head and Neck Squamous Cell Carcinoma

“…Due to the higher expression of MUC1 in OSCC than in normal mucosal tissues, it is considered a reliable biomarker for the diagnosis of OSCC. Silencing the MUC1 gene could induce apoptosis and inhibit the proliferation, invasion, migration and EMT of OSCC cells 123 , 124 . T]his membrane-localized glycoprotein is overexpressed and aberrantly glycosylated in most epithelial cancers 125 , and the aberrant glycosylation of MUC1 might result in the shortening of the sugar chain, causing the exposure of hidden antigens; the hidden antigens usually have peptidic and carbohydrate properties, making the MUC1 epitope tumor-specific 126 .…”