Changes in Protein Glycosylation in Head and Neck Squamous Cell Carcinoma

Liao, Chengcheng; An, Jiaxing; Tan, Zhangxue; Xu, Fangping; Liu, Jianguo; Wang, Qian

doi:10.7150/jca.51604

Cited by 25 publications

(19 citation statements)

References 137 publications

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“…Squamous cell carcinoma (SCC) accounts for most esophageal cancers (upper esophagus) 25 , more than 90% of cervical cancers 26 , 90% of head and neck cancers 27 , 30% of non-small-cell lung cancers (NSCLCs) 28 and 20% of skin cancers 29 . Head and neck squamous cell carcinoma (HNSCC), esophageal squamous cell carcinoma (ESCC), NSCLC and cutaneous squamous cell carcinoma (cSCC) are prone to metastasis, recurrence and invasion and have low 5-year survival rates 30 , 31 , 32 , 33 . HNSCC is prone to cervical lymph node and lung metastasis, and its 5-year survival rate is only 43% 32 .…”

Section: Introductionmentioning

confidence: 99%

“…Head and neck squamous cell carcinoma (HNSCC), esophageal squamous cell carcinoma (ESCC), NSCLC and cutaneous squamous cell carcinoma (cSCC) are prone to metastasis, recurrence and invasion and have low 5-year survival rates 30 , 31 , 32 , 33 . HNSCC is prone to cervical lymph node and lung metastasis, and its 5-year survival rate is only 43% 32 . The 5-year survival rate for esophageal cancer is less than 13% 30 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Partial EMT in Squamous Cell Carcinoma: A Snapshot

Liao¹,

Wang²,

An³

et al. 2021

Int. J. Biol. Sci.

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In the process of cancer EMT, some subgroups of cancer cells simultaneously exhibit both mesenchymal and epithelial characteristics, a phenomenon termed partial EMT (pEMT). pEMT is a plastic state in which cells coexpress epithelial and mesenchymal markers. In squamous cell carcinoma (SCC), pEMT is regulated, and the phenotype is maintained via the HIPPO pathway, NOTCH pathway and TGF-β pathways and by microRNAs, lncRNAs and the cancer microenvironment (CME); thus, SCC exhibits aggressive tumorigenic properties and high stemness, which leads collective migration and therapy resistance. Few studies have reported therapeutic interventions to address cells that have undergone pEMT, and this approach may be an effective way to inhibit the plasticity, drug resistance and metastatic potential of SCC.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Partial EMT in Squamous Cell Carcinoma: A Snapshot

Liao¹,

Wang²,

An³

et al. 2021

Int. J. Biol. Sci.

Self Cite

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“…There are extensive changes in the types and levels of glycosylation in tumor cells [32] . Some studies have shown that 7-H3 also has abnormal glycosylation in head and neck squamous cell carcinoma and oral squamous cell carcinoma [8,28] .…”

Section: Discussionmentioning

confidence: 99%

A4GALT mediated the glycosylation of B7-H3 in human colorectal cancer cell lines

Han

Chen³

et al. 2022

Preprint

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B7-H3 is one of the most important members of the B7/CD28 family, and its expression level is abnormally high in a variety of tumors. B7-H3 inhibits T cell activation via binding to the corresponding receptors on T cells, thereby mediating tumor immune escape. Glycosylation is a common post-translational modification of proteins, which plays an essential role in protein expression patterns and biological functions. Current evidence has shown that the abnormal glycosylation of B7-H3 in tumors is of great significance for protein expression and ligand-receptor binding. Therefore, in-depth exploration of the underlying mechanism of glycosylation modification of B7-H3 is expected to provide new insights for tumor immunotherapy. To investigate the underlying mechanism of glycosyltransferase-mediated glycosylation of B7-H3. Firstly, the CHIPBase database was used to screen glycosyltransferases with a high correlation with the protein expression of B7-H3. Then their siRNAs were designed and synthesized to transfect into cells, and the western blotting assay was performed for further screening. Secondly, the siRNA and overexpression plasmid of the screened glycosyltransferase were respectively transfected into cells to verify the effect on the expression level of B7-H3. Thirdly, the effect of glycosyltransferase on the expression level of B7-H3 was explored by changing its substrate level. Finally, the co-immunoprecipitation experiment was conducted to verify whether protein binding existed between B7-H3 and the glycosyltransferase. The glycosyltransferase called A4GALT had the highest correlation with the protein expression of B7-H3. After knocking down or overexpressing A4GALT, the protein expression level of B7-H3 both changed significantly. When knocked down GALT to reduce the galactosyl donors, B7-H3 was significantly down-regulated. And B7-H3 was up-regulated while increasing the galactose in the medium. In addition, the Co-immunoprecipitation experiment proved that there was protein binding between B7-H3 and A4GALT. A4GALT can positively regulate the expression of B7-H3, and changing the level of galactosyl donors can also positively regulate the expression of B7-H3. There is a protein interaction between A4GALT and B7-H3.

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“…thymoma viral oncogene homolog (PI3K/AKT), and JAK/STAT pathways. These pathways are involved in the proliferation, differentiation, migration and apoptosis of certain cells (69)(70)(71). The sequence of human epidermal growth factor (EGF) is similar to that of human SPINK1, and SPINK1 has 50% gene sequence homology with EGF (72,73).…”

Section: Spink1 Regulates the Egfr/mapk Signaling Pathway In Tumorsmentioning

confidence: 99%

SPINKs in Tumors: Potential Therapeutic Targets

Liao

Wang

et al. 2022

Front. Oncol.

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The serine protease inhibitor Kazal type (SPINK) family includes SPINK1-14 and is the largest branch in the serine protease inhibitor family. SPINKs play an important role in pancreatic physiology and disease, sperm maturation and capacitation, Nager syndrome, inflammation and the skin barrier. Evidence shows that the unregulated expression of SPINK1, 2, 4, 5, 6, 7, and 13 is closely related to human tumors. Different SPINKs exhibit various regulatory modes in different tumors and can be used as tumor prognostic markers. This article reviews the role of SPINK1, 2, 4, 5, 6, 7, and 13 in different human cancer processes and helps to identify new cancer treatment targets.

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Changes in Protein Glycosylation in Head and Neck Squamous Cell Carcinoma

Cited by 25 publications

References 137 publications

Partial EMT in Squamous Cell Carcinoma: A Snapshot

Partial EMT in Squamous Cell Carcinoma: A Snapshot

A4GALT mediated the glycosylation of B7-H3 in human colorectal cancer cell lines

SPINKs in Tumors: Potential Therapeutic Targets

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