mTORC2 regulates hierarchical micro/nano topography‐induced osteogenic differentiation via promoting cell adhesion and cytoskeletal polymerization

Gao, Qian; Hou, Yuxuan; Li, Zhe; Hu, Jinyang; Huo, Dawei; Zheng, Huimin; Zhang, Junjiang; Yao, Xiaoyu; Gao, Rui; Wu, Xudong; Sui, Lei

doi:10.1111/jcmm.16672

Cited by 13 publications

(14 citation statements)

References 65 publications

(139 reference statements)

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“…44 Recent studies have shown that mTORC2 is also involved in regulating autophagy, cell senescence, and induction of osteogenic differentiation. 48,49 In addition, studies have shown that mTORC3 affects the proliferation of tumour cells through 4E-BP1 35 ; however, the underlying regulatory mechanism remains elusive, warranting in-depth future investigations (Figure 2).…”

Section: Signal Transduction Of Mtor Signalling Pathwaymentioning

confidence: 99%

“…Studies have shown that mTORC1 can downregulate PI3K signalling via S6K1, resulting in mTORC2 inactivation, suggesting a potential feedback control loop between mTORC1 and mTORC2 44 . Recent studies have shown that mTORC2 is also involved in regulating autophagy, cell senescence, and induction of osteogenic differentiation 48,49 . In addition, studies have shown that mTORC3 affects the proliferation of tumour cells through 4E‐BP1 35 ; however, the underlying regulatory mechanism remains elusive, warranting in‐depth future investigations (Figure 2).…”

Section: Signal Transduction Of Mtor Signalling Pathwaymentioning

confidence: 99%

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Emerging role and therapeutic implication of mTOR signalling in intervertebral disc degeneration

et al. 2022

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Intervertebral disc degeneration (IDD), an important cause of chronic low back pain (LBP), is considered the pathological basis for various spinal degenerative diseases. A series of factors, including inflammatory response, oxidative stress, autophagy, abnormal mechanical stress, nutritional deficiency, and genetics, lead to reduced extracellular matrix (ECM) synthesis by intervertebral disc (IVD) cells and accelerate IDD progression. Mammalian target of rapamycin (mTOR) is an evolutionarily conserved serine/threonine kinase that plays a vital role in diverse degenerative diseases. Recent studies have shown that mTOR signalling is involved in the regulation of autophagy, oxidative stress, inflammatory responses, ECM homeostasis, cellular senescence, and apoptosis in IVD cells. Accordingly, we reviewed the mechanism of mTOR signalling in the pathogenesis of IDD to provide innovative ideas for future research and IDD treatment.

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Section: Signal Transduction Of Mtor Signalling Pathwaymentioning

confidence: 99%

Section: Signal Transduction Of Mtor Signalling Pathwaymentioning

confidence: 99%

Emerging role and therapeutic implication of mTOR signalling in intervertebral disc degeneration

et al. 2022

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“…The combined use of acid etching and sand blasting is Sandblasted, Large-grit, and Acid-etching (SLA), which is currently the most widely used implant surface treatment method (Li and Wang, 2020;Souza et al, 2020). Alkali-heat treatment can also be combined with micro-scale surface modification such as sandblasting to form a micro-nano hybrid structure (Zhuang et al, 2014;Gao et al, 2021;Hu et al, 2021). Anodization is an electrochemical method of surface modification on metal surfaces.…”

Section: Acid/alkali Treatment and Anodization Treatmentmentioning

confidence: 99%

Surface Modification Techniques to Produce Micro/Nano-scale Topographies on Ti-Based Implant Surfaces for Improved Osseointegration

Hou

Zhao

et al. 2022

Front. Bioeng. Biotechnol.

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Titanium and titanium alloys are used as artificial bone substitutes due to the good mechanical properties and biocompatibility, and are widely applied in the treatment of bone defects in clinic. However, Pure titanium has stress shielding effect on bone, and the effect of titanium-based materials on promoting bone healing is not significant. To solve this problem, several studies have proposed that the surface of titanium-based implants can be modified to generate micro or nano structures and improve mechanical properties, which will have positive effects on bone healing. This article reviews the application and characteristics of several titanium processing methods, and explores the effects of different technologies on the surface characteristics, mechanical properties, cell behavior and osseointegration. The future research prospects in this field and the characteristics of ideal titanium-based implants are proposed.

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“…influences osteogenic differentiation via various downstream molecular pathways following integrin signaling, reorganization of the actin cytoskeleton, and nuclear translocation/transcription [ 10 , 178 , 179 ]. These include several canonical pathways such as FAK [ 7 ], ERK [ 7 , 179 ], phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) [ 179 ], Rho-associated kinase (Rho-ROCK) [ 179 , 180 ], autophagy-mediated signaling between Yes-associated protein (YAP) and β-catenin [ 181 ], mammalian target of rapamycin complex with Rictor (mTORC2) [ 182 ], and Wnt/β-catenin [ 183 ] signaling pathways. Recently, Lv et al [ 184 ] revealed the epigenetic mechanism of nanotube-guided osteogenic differentiation of MSCs and that changes in cell adhesion and cytoskeleton reorganization are linked with epigenetic alterations.…”

Section: Tailoring Osteoinduction With Anodic Tio 2 Nanotubesmentioning

confidence: 99%

Anodic TiO2 Nanotubes: Tailoring Osteoinduction via Drug Delivery

et al. 2021

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TiO2 nanostructures and more specifically nanotubes have gained significant attention in biomedical applications, due to their controlled nanoscale topography in the sub-100 nm range, high surface area, chemical resistance, and biocompatibility. Here we review the crucial aspects related to morphology and properties of TiO2 nanotubes obtained by electrochemical anodization of titanium for the biomedical field. Following the discussion of TiO2 nanotopographical characterization, the advantages of anodic TiO2 nanotubes will be introduced, such as their high surface area controlled by the morphological parameters (diameter and length), which provides better adsorption/linkage of bioactive molecules. We further discuss the key interactions with bone-related cells including osteoblast and stem cells in in vitro cell culture conditions, thus evaluating the cell response on various nanotubular structures. In addition, the synergistic effects of electrical stimulation on cells for enhancing bone formation combining with the nanoscale environmental cues from nanotopography will be further discussed. The present review also overviews the current state of drug delivery applications using TiO2 nanotubes for increased osseointegration and discusses the advantages, drawbacks, and prospects of drug delivery applications via these anodic TiO2 nanotubes.

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mTORC2 regulates hierarchical micro/nano topography‐induced osteogenic differentiation via promoting cell adhesion and cytoskeletal polymerization

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 13 publications

References 65 publications

Emerging role and therapeutic implication of mTOR signalling in intervertebral disc degeneration

Emerging role and therapeutic implication of mTOR signalling in intervertebral disc degeneration

Surface Modification Techniques to Produce Micro/Nano-scale Topographies on Ti-Based Implant Surfaces for Improved Osseointegration

Anodic TiO2 Nanotubes: Tailoring Osteoinduction via Drug Delivery

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