MtDNA variation among subspecies of Apis cerana using restriction fragment length polymorphism

Deowanish, Sureerat; Nakamura, Jun; Matsuka, Mitsuo; Kimura, Kiyoshi

doi:10.1051/apido:19960508

Cited by 33 publications

(26 citation statements)

References 4 publications

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“…A non-coding region of the Apis mitochondrial genome (Cornuet et al, 1991) has been used extensively in studies of honey bee biogeography and population biology (for example, Garnery et al, 1992Garnery et al, , 1995Garnery et al, , 1998Oldroyd et al, 1995;Moritz et al, 1994;Deowanish et al, 1996;Smith and Hagen, 1996;de la Rúa et al, 1998de la Rúa et al, , 2000Deowanish et al, 1998;Franck et al, 1998Franck et al, , 2000aFranck et al, ,b, 2001Sheppard et al, 1999;Sihanuntavong et al, 1999;Smith and Hagen, 1999;Palmer et al, 2000;Smith et al, 2000Smith et al, , 2003Smith et al, , 2004Smith et al, , 2005Warrit et al, 2006, and many more). Few data of this nature are available for Chinese A. cerana.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA diversity of ChineseApis cerana

2007

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-DNA sequence diversity in a non-coding portion of the mitochondrial genome was investigated in samples of Apis cerana from 47 locations in China. Nine haplotypes (mitochondrial genotypes) were found: Japan1, Japan2, Korea4, and Cambodia2, which were previously reported from other populations, and China1−5, which are new. All nine sequences belong to the Mainland mitochondrial lineage, and none differs from the Japan1 haplotype by more than a single base substitution and/or a single insertion/deletion. Japan1 is the most common haplotype, making up 39 of 49 sequences. Haplotype diversity was 0.4 (s.d. 0.089) and nucleotide diversity was 0.00569 (s.d. 0.00154). By both measures the Chinese samples were more diverse than those from Japan and Thailand, similar to populations from Pakistan, Burma and Korea, and less diverse than samples from Indochina (Laos-Cambodia-Vietnam).mitochondrial DNA / Apis cerana / China / phylogeography

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Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA diversity of ChineseApis cerana

2007

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“…However, multivariate morphometric analyses of the former lag behind the latter and a recent survey of the literature shows that infraspecific classification and population structure in Apis cerana are fraught with difficulties (Hepburn et al, 2001). A beginning toward a resolution of this disparity is apparent from the recent publication of several such analyses for different parts of Asia (Sasaki, 1994;Deowanish et al, 1996;Damus and Otis, 1997;Sylvester et al, 1998;Sihanuntavong et al, 1999;de la Rúa et al, 2000;Smith et al, 2000;Tilde et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Morphometric analysis of Apis cerana populations in the southern Himalayan region

et al. 2001

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-Multivariate analyses of morphometric traits of Apis cerana were measured from 3704 individual workers of 279 colonies from 64 localities with an average sampling distance resolution of 50 km along a 2200 km transect in the southern Himalayan region bordered by Pakistan to the west and Myanmar to the east. Factor and discriminant function analyses revealed four major morphoclusters (= unnamed subspecies), two of which are further subdivisable into three biometric subgroups each. These morphoclusters can only be partially integrated into any current subspecific nomenclature available for Apis cerana. Morphocluster separation is related to physiographic differences which create a partial temporal reproductive isolation associated with altitude. High variance domains occur at the edges of morphocluster and biometric groupings. The bees decrease in size from west to east but increase in size with increasing altitude. Apis cerana / honeybees / morphoclusters / population genetics / southern Himalayas

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“…Population genetic studies of the Asian honey bee (Apis cerana) in Thailand using mitochondrial DNA and microsatellite polymorphism revealed the biogeographical transition area between Mainland and Sundaland populations located at the Kra ecotone (at Tup Sa Kae, Prachup Kiri Khan, 11°31'N, 99°35'E, and Bang Sapan, Prachup Kiri Khan, 11°24'N, 99°31'E; Deowanish et al, 1996;Hagen, 1996, 1999;Sihanuntavong et al, 1999;Warrit et al, 2006). A pattern of geographic differentiation of the giant honey bee (Apis dorsata) in Thailand between north-to-central and peninsular Thailand populations was also noticed found, based on microsatellite analysis (Insuan et al, 2007).…”

Section: Application Of Cutc1 For Determining Genetic Population Strumentioning

confidence: 99%

Development of a species-diagnostic marker and its application for population genetics studies of the stingless bee Trigona collina in Thailand

Theeraapisakkun¹,

Klinbunga²,

Sittipraneed³

2010

Genet. Mol. Res.

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ABSTRACT. A molecular maker for authenticating species origin of the stingless bee (Trigona collina) was developed. Initially, amplified fragment length polymorphism analysis was made of 11 stingless bee species using 64 primer combinations. A 316-bp band found only in T. collina was cloned and sequenced. A primer pair (CUTc1-F/R) was designed and tested for species-specificity in 15 stingless bee species (239 nests). The expected 259-bp fragment was consistently amplified in all T. collina individuals (134/134 nests, 100%). Cross-species amplification was observed in T. pagdeni (43/51 nests; 84.3%), but not in other species. SSCP analysis of CUTc1 unambiguously differentiated T. collina from T. pagdeni. CUTc1 generated three genotypes in Thai T. collina (134 nests). An AA (259/259 bp) genotype was found in all stingless bees from the north (21 nests) and northeast (32 nests), and 23/28 nests from the Central region, whereas a BB (253/253 bp) genotype was observed in most samples from peninsular Thailand (42/53 nests). Heterozygotes exhibiting the AB (253/259 bp) genotype were observed in 5 of 28 nests from Prachuap Khiri Khan located slightly above the Kra ecotone and 11 of 53 nests originated further south of the Kra ecotone. Genotype distribution patterns of CUTc1 clearly indicated intraspecific population differentiation of Thai T. collina.

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MtDNA variation among subspecies of Apis cerana using restriction fragment length polymorphism

Cited by 33 publications

References 4 publications

Mitochondrial DNA diversity of ChineseApis cerana

Mitochondrial DNA diversity of ChineseApis cerana

Morphometric analysis of Apis cerana populations in the southern Himalayan region

Development of a species-diagnostic marker and its application for population genetics studies of the stingless bee Trigona collina in Thailand

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