mt-COX1, mt-ND1 and CREBP are indicators of intramuscular fat content in Hanwoo (Korean cattle)

Lee, Seung Hwan; Kim, Seung-Chang; Choi, Bong-Hwan; Lim, Dajeong; Kim, Nam Kuk; Lee, Jun Heon; Kim, Oun Hyun; Lee, Chang‐Soo; Kim, Hyeong Cheol; Yang, Boh Suk; Hong, Sung Kee

doi:10.1016/j.livsci.2012.03.003

Cited by 5 publications

(4 citation statements)

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“…While associations of the MT-CO1 gene with intramuscular fat in animals have been reported [35] , no significant findings have been reported so far for the MT-CO3 gene with BMI, nor with other BMI-related phenotypes. Liu et al [37] suggested an effect of mtSNPs, including MT-CO2 , MT-ND1 , and MT-ND2 genes, on BMI but this effect failed to reach significance after adjusting for multiple testing.…”

Section: Discussionmentioning

confidence: 90%

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Mitochondrial Genetic Variants Identified to Be Associated with BMI in Adults

et al. 2014

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It has been suggested that mitochondrial dysfunction plays a role in metabolic disorders including obesity, diabetes, and hypertension. The fact that mitochondrial defects can be accumulated over time as a normal part of aging may explain why some individuals can eat all sorts of foods and remain at normal weight while they are young. However, around the fourth decade of life there is a trend towards “middle-age spread” with weight gain and the body's decreasing ability to metabolize calories efficiently. To test the hypothesis that mitochondrial variants are associated with BMI in adults, we analyzed a total number of 984 mitochondrial single nucleotide polymorphisms (mtSNPs) in a sample of 6,528 individuals participating in the KORA studies. To assess mtSNP association while taking heteroplasmy into account we used the raw signal intensity values measured on the microarray and applied linear regression. Significant results were obtained for 2 mtSNPs located in the Cytochrome c oxidase subunit genes (MT-CO1: Padjusted = 0.0140 and MT-CO3: Padjusted = 0.0286) and 3 mtSNPs located in the NADH dehydrogenase subunit genes (MT-ND1, MT-ND2 and MT-ND4L: Padjusted = 0.0286). Polymorphisms located in the MT-CO3 and MT-ND4L genes have not been associated with BMI or related phenotypes in the past. Our results highlight the importance of the mitochondrial genome among the factors that contribute to the risk of high BMI. Focusing on mitochondrial variants may lead to further insights regarding effects of existing medications, or even to the development of innovative treatments.

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Section: Discussionmentioning

confidence: 90%

“…MT-ND1 and MT-ND2 genes were previously reported to be related with body fat mass and intramuscular fat in animals [23] , [34] , [35] . The significant findings in our study at mt3336T→G ( MT-ND1 ) and mt4851C→T ( MT-ND2 ) have associated effect sizes estimated to be −0.23 and −1.11, respectively.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Genetic Variants Identified to Be Associated with BMI in Adults

et al. 2014

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“…In 2012, Lee et al . reported that the expression of the cAMP responsive element binding protein ( CREB1 ) gene is higher in muscle with high IMF content in Hanwoo [49]. CREB1 is a transcription factor containing a basic leucine zipper.…”

Section: Resultsmentioning

confidence: 99%

Whole-genome resequencing of Hanwoo (Korean cattle) and insight into regions of homozygosity

et al. 2013

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BackgroundHanwoo (Korean cattle), which originated from natural crossbreeding between taurine and zebu cattle, migrated to the Korean peninsula through North China. Hanwoo were raised as draft animals until the 1970s without the introduction of foreign germplasm. Since 1979, Hanwoo has been bred as beef cattle. Genetic variation was analyzed by whole-genome deep resequencing of a Hanwoo bull. The Hanwoo genome was compared to that of two other breeds, Black Angus and Holstein, and genes within regions of homozygosity were investigated to elucidate the genetic and genomic characteristics of Hanwoo.ResultsThe Hanwoo bull genome was sequenced to 45.6-fold coverage using the ABI SOLiD system. In total, 4.7 million single-nucleotide polymorphisms and 0.4 million small indels were identified by comparison with the Btau4.0 reference assembly. Of the total number of SNPs and indels, 58% and 87%, respectively, were novel. The overall genotype concordance between the SNPs and BovineSNP50 BeadChip data was 96.4%. Of 1.6 million genetic differences in Hanwoo, approximately 25,000 non-synonymous SNPs, splice-site variants, and coding indels (NS/SS/Is) were detected in 8,360 genes. Among 1,045 genes containing reliable specific NS/SS/Is in Hanwoo, 109 genes contained more than one novel damaging NS/SS/I. Of the genes containing NS/SS/Is, 610 genes were assigned as trait-associated genes. Moreover, 16, 78, and 51 regions of homozygosity (ROHs) were detected in Hanwoo, Black Angus, and Holstein, respectively. ‘Regulation of actin filament length’ was revealed as a significant gene ontology term and 25 trait-associated genes for meat quality and disease resistance were found in 753 genes that resided in the ROHs of Hanwoo. In Hanwoo, 43 genes were located in common ROHs between whole-genome resequencing and SNP chips in BTA2, 10, and 13 coincided with quantitative trait loci for meat fat traits. In addition, the common ROHs in BTA2 and 16 were in agreement between Hanwoo and Black Angus.ConclusionsWe identified 4.7 million SNPs and 0.4 million small indels by whole-genome resequencing of a Hanwoo bull. Approximately 25,000 non-synonymous SNPs, splice-site variants, and coding indels (NS/SS/Is) were detected in 8,360 genes. Additionally, we found 25 trait-associated genes for meat quality and disease resistance among 753 genes that resided in the ROHs of Hanwoo. These findings will provide useful genomic information for identifying genes or casual mutations associated with economically important traits in cattle.

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“…The TUBA1B gene has been shown to play an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance ( Kapeller et al, 1995 ). Cytochrome c oxidase and NADH dehydrogenase showed increased gene expression levels during adipocyte differentiation and intramuscular fat deposition, suggesting that these genes play important roles in fat development ( Lee et al, 2012 ). Deficiencies in COX1 and COX2 gene activity have been correlated with a number of human aging diseases such as Alzheimer’s and muscle atrophy ( Barrientos et al, 2002 ).…”

Section: Resultsmentioning

confidence: 99%

Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose

Zhang

Long

et al. 2016

PeerJ

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A better understanding of the control of lipogenesis is of critical importance for both human and animal physiology. This requires a better knowledge of the changes of gene expression during the process of adipose tissue development. Thus, the objective of the current study was to determine the effects of development on subcutaneous adipose tissue gene expression in growing and adult pigs. Here, we present a comprehensive investigation of mRNA transcriptomes in porcine subcutaneous adipose tissue across four developmental stages using digital gene expression profiling. We identified 3,274 differential expressed genes associated with oxidative stress, immune processes, apoptosis, energy metabolism, insulin stimulus, cell cycle, angiogenesis and translation. A set of universally abundant genes (ATP8, COX2, COX3, ND1, ND2, SCD and TUBA1B) was found across all four developmental stages. This set of genes may play important roles in lipogenesis and development. We also identified development-related gene expression patterns that are linked to the different adipose phenotypes. We showed that genes enriched in significantly up-regulated profiles were associated with phosphorylation and angiogenesis. In contrast, genes enriched in significantly down-regulated profiles were related to cell cycle and cytoskeleton organization, suggesting an important role for these biological processes in adipose growth and development. These results provide a resource for studying adipose development and promote the pig as a model organism for researching the development of human obesity, as well as being used in the pig industry.

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mt-COX1, mt-ND1 and CREBP are indicators of intramuscular fat content in Hanwoo (Korean cattle)

Cited by 5 publications

References 24 publications

Mitochondrial Genetic Variants Identified to Be Associated with BMI in Adults

Mitochondrial Genetic Variants Identified to Be Associated with BMI in Adults

Whole-genome resequencing of Hanwoo (Korean cattle) and insight into regions of homozygosity

Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose

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