Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose

Abstract

A better understanding of the control of lipogenesis is of critical importance for both human and animal physiology. This requires a better knowledge of the changes of gene expression during the process of adipose tissue development. Thus, the objective of the current study was to determine the effects of development on subcutaneous adipose tissue gene expression in growing and adult pigs. Here, we present a comprehensive investigation of mRNA transcriptomes in porcine subcutaneous adipose tissue across four developmental stages using digital gene expression profiling. We identified 3,274 differential expressed genes associated with oxidative stress, immune processes, apoptosis, energy metabolism, insulin stimulus, cell cycle, angiogenesis and translation. A set of universally abundant genes (ATP8, COX2, COX3, ND1, ND2, SCD and TUBA1B) was found across all four developmental stages. This set of genes may play important roles in lipogenesis and development. We also identified development-related gene expression patterns that are linked to the different adipose phenotypes. We showed that genes enriched in significantly up-regulated profiles were associated with phosphorylation and angiogenesis. In contrast, genes enriched in significantly down-regulated profiles were related to cell cycle and cytoskeleton organization, suggesting an important role for these biological processes in adipose growth and development. These results provide a resource for studying adipose development and promote the pig as a model organism for researching the development of human obesity, as well as being used in the pig industry.

Identification of lncRNAs and Genes Responsible for Fatness and Fatty Acid Composition Traits between the Tibetan and Yorkshire Pigs

“…It has been surveyed that fat mass will be augmented in response to excess caloric intake leading to obesity and further to inflammatory signaling [29]. As part of the immune system, adipose tissue had been identified to be involved in inflammatory response which also inversely participated in obesity and insulin resistance [30]. GO terms, included “leukocyte migration,” “activation of immune response,” and “macrophage differentiation,” were enriched by upregulated DEGs (Figure 3 and Table S4).…”

Comparative proteomics analysis reveals the difference during antler regeneration stage between red deer and sika deer

“…In this case, ATP6 could be the key enzyme involved in ATP production during antler development, whereas ATP8 may take over the roles of ATP6 in red deer. In addition, ATP8 , which plays important roles in lipogenesis, was found during the stage of porcine adipose tissue development (Zhang et al, 2016). Weiss et al (2012) reported that ATP8 mutation led to mitochondrial ROS generation with dysregulation of mitochondrial OxPhos system.…”

Dynamic Organization of lncRNA and Circular RNA Regulators Collectively Controlled Cardiac Differentiation in Humans

“…In this current study, we used the commonly used method (ANOVA) to identify the differentially expressed mRNAs, lncRNAs and circRNAs during heart differentiation ( Yu et al, 2014 ; Zhang et al, 2016 ). There are a number of statistical tests specifically designed for RNA-Seq data, such as DESeq ( Love et al, 2014 ) and edgeR ( Robinson et al, 2010 ).…”