MsCYS1, a developmentally-regulated cystatin from alfalfa

Rivard, Daniel; Girard, Cécile; Anguenot, Raphaël; Vézina, Louis‐P.; Trépanier, Sonia; Michaud, Dominique

doi:10.1016/j.plaphy.2007.03.028

Cited by 19 publications

(14 citation statements)

References 44 publications

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“…However, there is increasing evidence that protease inhibitors also participate in the regulation of endogenous proteases (Rivard et al 2007;Prins et al 2008). Cys-proteases are involved in deposition and degradation of storage proteins, senescence, programmed cell death, and stress signaling (Grudkowska and Zagdajska 2004;van der Hoorn 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Proteolysis is a complex process involving many diVerent pathways and cellular compartments. Proteases in vivo are inhibited by endogenous inhibitors (Solomon et al 1999;Rivard et al 2007) or by compartmentation to prevent indiscriminant breakdown of proteins essential for metabolism (Wang et al 2007). Protein degradation by proteases supplies amino acids for protein synthesis by degrading abnormal/misfolded proteins and for translocation to developing plants (Michaud et al 1994) or seedlings (Sheokand et al 2005) in the case of vegetative propagules or seeds, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Developmentally linked changes in proteases and protease inhibitors suggest a role for potato multicystatin in regulating protein content of potato tubers

Weeda

Kumar

Knowles

2009

Planta

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The soluble protein fraction of fully developed potato (Solanum tuberosum L.) tubers is dominated by patatin, a 40 kD storage glycoprotein, and protease inhibitors. Potato multicystatin (PMC) is a multidomain Cys-type protease inhibitor. PMC effectively inhibits degradation of patatin by tuber proteases in vitro. Herein we show that changes in PMC, patatin concentration, activities of various proteases, and their gene expression are temporally linked during tuber development, providing evidence that PMC has a role in regulating tuber protein content in vivo. PMC was barely detectable in non-tuberized stolons. PMC transcript levels increased progressively during tuberization, concomitant with a 40-fold increase in PMC concentration (protein basis) as tubers developed to 10 g fresh wt. Further increases in PMC were comparatively modest (3.7-fold) as tubers developed to full maturity (250 g). Protease activity declined precipitously as PMC levels increased during tuberization. Proteolytic activity was highest in non-tuberized stolons and fell substantially through the 10-g fresh wt stage. Cys-type proteases dominated the pre-tuberization and earliest stages of tuber development. Increases in patatin transcript levels during tuberization were accompanied by a notable lag in patatin accumulation. Patatin did not begin to accumulate substantially on a protein basis until tubers had reached the 10-g stage, wherein protease activity had been inhibited by approximately 60%. These results indicate that a threshold level of PMC (ca. 3 microg tuber(-1), 144 ng mg(-1) protein) is needed to favor patatin accumulation. Collectively, these results are consistent with a role for PMC in facilitating the accumulation of proteins in developing tubers by inhibiting Cys-type proteases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Developmentally linked changes in proteases and protease inhibitors suggest a role for potato multicystatin in regulating protein content of potato tubers

Weeda

Kumar

Knowles

2009

Planta

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“…Much interest in protease inhibitors has focussed on their use to improve insect resistance in crop plants (Ferry et al 2003) or to improve stability of heterologously expressed recombinant proteins (Rivard et al 2007). More recently the role of cysteine protease inhibitor during cold stress and anaerobiosis has been investigated (Gaddour et al 2001;Van der Vyver et al 2003) and a growing body of evidence suggests developmentally-regulated phytocystatins function to mediate fine control of endogenous cysteine protease activity during development (Rivard et al 2007). Proteolysis in general in cells plays a vital role in protein homeostasis as well as controlling enzyme activity and signal transduction components associated with plant growth and development (Dreher and Callis 2007).…”

Section: Sdg20smentioning

confidence: 99%

The resurrection plant Sporobolus stapfianus: An unlikely model for engineering enhanced plant biomass?

et al. 2010

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The resurrection grass Sporobolus stapfianus Gandoger can rapidly recover from extended periods of time in the desiccated state (water potential equilibrated to 2% relative humidity) (Gaff and Ellis, Bothalia 11:305-308 1974; Gaff and Loveys, Transactions of the Malaysian Society of Plant Physiology 3:286-287 1993). Physiological studies have been conducted in S. stapfianus to investigate the responses utilised by these desiccationtolerant plants to cope with severe water-deficit. In a number of instances, more recent gene expression analyses in S. stapfianus have shed light on the molecular and cellular mechanisms mediating these responses. S. stapfianus is a versatile research tool for investigating desiccation-tolerance in vegetative grass tissue, with several useful characteristics for differentiating desiccation-tolerance adaptive genes from the many dehydration-responsive genes present in plants. A number of genes orthologous to those isolated from dehydrating S. stapfianus have been successfully used to enhance drought and salt tolerance in model plants as well as important crop species. In addition to the ability to desiccate and rehydrate successfully, the survival of resurrection plants in regions experiencing short sporadic rainfall events may depend substantially on the ability to tightly down-regulate cell division and cell wall loosening activities with decreasing water availability and then grow rapidly after rainfall while water is plentiful. Hence, an analysis of gene transcripts present in the desiccated tissue of resurrection plants may reveal important growth-related genes. Recent findings support the proposition that, as well as being a versatile model for devising strategies for protecting plants from water-loss, resurrection plants may be a very useful tool for pinpointing genes to target for enhancing growth rate and biomass production.

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“…Cystatins exist widely in many organisms from microbes to plants and animals. In plants, several roles have been attributed to these proteins including the control of endogenous cysteine protease in physiological and developmental processes such as the development of stem and leaves 4 , protein turnover during seed maturation, and germination [5][6][7] and programmed cell death 8 . Several lines of evidence suggest that plant cystatins are also responsive to abiotic stresses such as drought, salt, ABA and cold treatment and this helps plants to improve their ability to tolerate environmental stress [9][10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

Characterization of a cDNA encoding cystatin with antifungal activity from Siam tulip Curcuma alismatifolia

Porruan¹,

Chundet²,

Anuntalabhochai³

2013

ScienceAsia

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Cystatins are cysteine protease inhibitors involved in defence mechanisms against pests and pathogens. Here, the cystatin CaCPI gene was isolated from a cDNA library of the Siam tulip (Curcuma alismatifolia cv. Chiang Mai Pink was then transformed into Escherichia coli strain BL21-Star to produce a recombinant CaCPI protein. After induction with 1 mM IPTG, the cell lysate of E. coli-carrying pDEST17-CaCPI generated a CaCPI protein about 12 kDa in size as measured using SDS-PAGE. Pre-incubation of the 5-30 µM CaCPI protein samples with 5 µM papain is known to decrease papain activity. Antifungal activities of the purified recombinant CaCPI protein against phytopathogenic fungi were tested. The CaCPI protein could suppress mycelium growth of Fusarium oxysporum, Colletotrichum capsici, and Pyricularia grisea phytopathogenic fungi.

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MsCYS1, a developmentally-regulated cystatin from alfalfa

Cited by 19 publications

References 44 publications

Developmentally linked changes in proteases and protease inhibitors suggest a role for potato multicystatin in regulating protein content of potato tubers

Developmentally linked changes in proteases and protease inhibitors suggest a role for potato multicystatin in regulating protein content of potato tubers

The resurrection plant Sporobolus stapfianus: An unlikely model for engineering enhanced plant biomass?

Characterization of a cDNA encoding cystatin with antifungal activity from Siam tulip Curcuma alismatifolia

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