mRNA/lncRNA expression patterns and the function of fibrinogen‐like protein 2 in Meishan pig endometrium during the preimplantation phases

Abstract

Embryonic implantation involves a complex and well‐coordinated interaction between the developing conceptus and maternal uterus, and the preimplantation period has a major impact on litter size in pigs. The present study aimed to investigate the vital messenger RNAs (mRNAs) and long noncoding RNAs (lncRNAs) that regulate preimplantation in Meishan pigs. The enriched Gene Ontology terms were all related to “binding.” Furthermore, “ECM‐receptor interaction” was predicted as an important pathway that regulated the success of implantation. We speculated that the differentially expressed mRNAs S100A9, ANXA8, MUC16, and FGL2 and the differentially expressed lncRNAs TCONS_11206566, TCONS_09904861, and TCONS_1252933 may play vital roles in the process of implantation. Furthermore, this study verified that FGL2 was highly expressed on Day 12 of pregnancy, and we also investigated the function of FGL2 during preimplantation in vivo. In conclusion, this study provides useful information for further analyses of the molecular mechanisms of implantation in Chinese domestic pigs.

Small RNA-seq analysis of extracellular vesicles from porcine uterine flushing fluids during peri-implantation

“…The animal experiment has been described in Wang et al [60]. All experiments involving animals were Before every gilt was slaughtered, a warm shower to relax the pigs, then they were stunned with lowvoltage electric shock to reduce the pain and were exsanguinated by puncturing carotid artery to death.…”

“…Endometrial stromal cells were further removed by 0.25% trypsin without edetic acid disodium salt (EDTA) after 2-4 d. The epithelial cells were then trypsinized with 0.25% trypsin-EDTA and placed on Cell Culture Flask (Corning, NY, USA) for subsequent experiments. Isolation and culture of porcine primary EECs referred to pervious research [60,62]. Detailed methods have been published [63].These cells were cultured in DMEM-F12 (Gibco) supplemented with insulin (0.1 Units/mL; Sigma-Aldrich, German), glutamine (2 mM, Sigma-Aldrich), 1% penicillin-streptomycin and 5% fetal bovine serum (Gibco).…”

Small RNA-seq analysis of exosomes from porcine uterine luminal fluid during peri-implantation

“…The animal experiment has been described in Wang et al [41]. All experiments involving animals were carried out in accordance with regulations (No.…”

“…The pellets mainly containing endometrial epithelial cells (EECs) were suspended in Dulbecco's modified Eagle's medium/F-12 (DMEM/F12; 1:1) medium (Gibco, NY, USA) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Gibco) and cultured in 37°C and 5% CO 2 incubator. Endometrial stromal cells were further removed by 0.25% trypsin without edetic acid disodium salt (EDTA) after 2-4 d. Isolation and culture of porcine primary EECs referred to pervious research [41,43].…”

Spatial Transcriptomic and miRNA Analyses Revealed Genes Involved in the Mesometrial-Biased Implantation in Pigs

“…A recent study conducted by using laser capture microdissection (LCM) determined the gene expression changes in different types of endometrial cells between day 12 of pregnant and non-pregnant cyclic gilts [9]. These findings identified genes and regulatory pathways that are associated with embryo implantation in pigs [10,11,12,13,14,15].…”