Small RNA-seq analysis of extracellular vesicles from porcine uterine flushing fluids during peri-implantation

Hua, Renwu; Wang, Yueying; Lian, Weisi; Li, Wenchao; Xue, Shuai; Kang, Tingting; Lei, Minggang

doi:10.1016/j.gene.2020.145117

Cited by 26 publications

(27 citation statements)

References 81 publications

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“…Of the 53 DA proteins identified in uEVs in our study, 32 were in common with at least one of these 5 studies. At the miRNA level, a comparison was performed with two studies of porcine uEVs 39 , 40 and one in goat 41 analyzing miRNAs in pregnant vs. control animals. An overlap of 81 miRNAs was found with the two pig studies, while 21 miRNAs with the goat study.…”

Section: Resultsmentioning

confidence: 99%

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Vegas

Hamdi

Podico

et al. 2022

Sci Rep

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In contrast to other domestic mammals, the embryo-derived signal(s) leading to maternal recognition of pregnancy (MRP) are still unknow in the mare. We hypothesize that these embryonic signals could be packed into uterine extracellular vesicles (uEVs), acting as multi-signal messengers between the conceptus and the maternal tract, and contributing to MRP. To unveil these signals, the RNA and protein cargos of uEVs isolated from uterine lavages collected from pregnant mares (P; day 10, 11, 12 and 13 after ovulation) and cyclic control mares (C; day 10 and 13 after ovulation) were analyzed. Our results showed a fine-tuned regulation of the uEV cargo (RNAs and proteins), by the day of pregnancy, the estrous cycle, and even the size of the embryo. A particular RNA pattern was identified with specific increase on P12 related to immune system and hormonal response. Besides, a set of proteins as well as RNAs was highly enriched in EVs on P12 and P13. Differential abundance of miRNAs was also identified in P13-derived uEVs. Their target genes were linked to down- or upregulated genes in the embryo and the endometrium, exposing their potential origin. Our study identified for first time specific molecules packed in uEVs, which were previously associated to MRP in the mare, and thus bringing added value to the current knowledge. Further integrative and functional analyses will help to confirm the role of these molecules in uEVs during MRP in the mare.

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Section: Resultsmentioning

confidence: 99%

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Vegas

Hamdi

Podico

et al. 2022

Sci Rep

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“…Hua and collaborators found small RNAs in swine uterine fluid-derived Evs of gilts on days 10, 13, and 18 of pregnancy that were involved in pathways of immunization, endometrial receptivity, embryo development, and implantation. They also highlighted a different expression of some miRNAs during the three phases of pregnancy, whose role as biomarkers to monitor pregnancy could be further studied [ 97 ].…”

Section: Evs As Biomarkersmentioning

confidence: 99%

Extracellular Vesicles in Veterinary Medicine

Moccia

Sammarco

Cavicchioli

et al. 2022

Animals

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Extracellular vesicles (EVs) are cell-derived membrane-bound vesicles involved in many physiological and pathological processes not only in humans but also in all the organisms of the eukaryotic and prokaryotic kingdoms. EV shedding constitutes a fundamental universal mechanism of intra-kingdom and inter-kingdom intercellular communication. A tremendous increase of interest in EVs has therefore grown in the last decades, mainly in humans, but progressively also in animals, parasites, and bacteria. With the present review, we aim to summarize the current status of the EV research on domestic and wild animals, analyzing the content of scientific literature, including approximately 220 papers published between 1984 and 2021. Critical aspects evidenced through the veterinarian EV literature are discussed. Then, specific subsections describe details regarding EVs in physiology and pathophysiology, as biomarkers, and in therapy and vaccines. Further, the wide area of research related to animal milk-derived EVs is also presented in brief. The numerous studies on EVs related to parasites and parasitic diseases are excluded, deserving further specific attention. The literature shows that EVs are becoming increasingly addressed in veterinary studies and standardization in protocols and procedures is mandatory, as in human research, to maximize the knowledge and the possibility to exploit these naturally produced nanoparticles.

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“…Subsequent bioinformatics analysis revealed that the miRNA enriched in the EVs involved in important pregnancy associated pathways such as immunomodulation, endometrial receptivity, implantation and embryo development. These studies add to the miRNA repository as well as serve as a resource to further investigate crosstalk at the maternal-fetal interface (138). As integrin family proteins play an important role in embryo implantation, previous study demonstrated that EVs in the bovine uterine flushings isolated on gd20 and 22 were able to upregulate VCAM1 expression in endometrial epithelial cells (139).…”

Section: Extracellular Vesicles In Porcine Pregnancymentioning

confidence: 99%

Insights Into Extracellular Vesicle/Exosome and miRNA Mediated Bi-Directional Communication During Porcine Pregnancy

et al. 2021

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Spontaneous fetal loss is one of the most important challenges that commercial pig industry is still facing in North America. Research over the decade provided significant insights into some of the associated mechanisms including uterine capacity, placental efficiency, deficits in vasculature, and immune-inflammatory alterations at the maternal-fetal interface. Pigs have unique epitheliochorial placentation where maternal and fetal layers lay in opposition without any invasion. This has provided researchers opportunities to accurately tease out some of the mechanisms associated with maternal-fetal interface adaptations to the constantly evolving needs of a developing conceptus. Another unique feature of porcine pregnancy is the conceptus derived recruitment of immune cells during the window of conceptus attachment. These immune cells in turn participate in pregnancy associated vascular changes and contribute toward tolerance to the semi-allogeneic fetus. However, the precise mechanism of how maternal-fetal cells communicate during the critical times in gestation is not fully understood. Recently, it has been established that bi-directional communication between fetal trophoblasts and maternal cells/tissues is mediated by extracellular vesicles (EVs) including exosomes. These EVs are detected in a variety of tissues and body fluids and their role has been described in modulating several physiological and pathological processes including vascularization, immune-modulation, and homeostasis. Recent literature also suggests that these EVs (exosomes) carry cargo (nucleic acids, protein, and lipids) as unique signatures associated with some of the pregnancy associated pathologies. In this review, we provide overview of important mechanisms in porcine pregnancy success and failure and summarize current knowledge about the unique cargo containing biomolecules in EVs. We also discuss how EVs (including exosomes) transfer their contents into other cells and regulate important biological pathways critical for pregnancy success.

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Small RNA-seq analysis of extracellular vesicles from porcine uterine flushing fluids during peri-implantation

Cited by 26 publications

References 81 publications

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Uterine extracellular vesicles as multi-signal messengers during maternal recognition of pregnancy in the mare

Extracellular Vesicles in Veterinary Medicine

Insights Into Extracellular Vesicle/Exosome and miRNA Mediated Bi-Directional Communication During Porcine Pregnancy

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