mRNA from porcine colostral and milk cells coding for specific antibodies in response to orally administered strains

Kortbeek-Jacobs, Netty; Donk, Hans van der

doi:10.1016/0165-2427(81)90004-0

Cited by 2 publications

(4 citation statements)

References 14 publications

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“…Although MaLN were rich in antigen-specific cells after Schedule IV, they expressed IgG, which may be of less importance in the local protection of neonatal piglets. Previous experiments have already indicated a prevalence of cells containing IgA-and IgMmRNA, specific for K88 antigen, in the colostrum of sows orally immunized according to Schedule V (Kortbeek-Jacobs and van der Donk, 1981). The experiments reported here complement these findings.…”

Section: Productive Capacitysupporting

confidence: 86%

“…The clear supernatants were collected for analysis by radioimmunoassays (RIA). In parallel assays for IgA, IgG, IgM and the total amount of K88 antibodies, translated in ovo, were conducted as described by Kortbeek-Jacobs and van der Donk (1981).…”

Section: In Ovo Translation Of Mrnamentioning

confidence: 99%

“…Knowledge of the mechanisms(s) by which the antibodies resulting from immunization enter the mammary secretions may contribute to the prevention of neonatal infectious diseases. We have already reported, that in sows' colostrum and milk, cells are present that contain mRNA coding for antibodies directed against intestinal antigens (Kortbeek-Jacobs and van der Donk, 1981). These committed cells, capable of producing Ag-specific immunoglobulins, are presumed to originate from the gut.…”

Section: Introductionmentioning

confidence: 99%

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The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow

Kortbeek-Jacobs

Kooten

Donk

et al. 1984

Veterinary Microbiology

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Kortbeek-Jacobs, J.M.C., van Kooten, P.J.S., van der Donk, J.A., van Dijk, J.E. and Rutten, V.P., 1984. The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow. Vet. Microbiol., 9: 287--299.Sows were immunized orally with live Escherichia coli according to various immunization schedules. Six pregnant gilts were used; 4 immunized at various intervals during the last month of gestation, 1 control immunized after parturition following suppression of lactation by weaning and 1 non-immunized control. The effect of oral vaccination on cell populations from lymphoid organs was studied. The in vitro proliferative responses of the cell populations to K88 antigen, anti-Ig sera and mitogens were used to demonstrate the distribution of sensitized lymphocytes over different lymphoid organs. The capacity of these cells to produce antigen-specific Ig was determined by in ovo translation of their mRNA. Oral administration of antigen resulted in the appearance of K88-positive cells in lymphoid organs. In lactating sows, sensitized cells preferentially occurred in the mammary lymph nodes, whereas after suppression of lactation such a distribution was not seen. A possible route of migration of sensitized lymphocytes is discussed in relation to the local immune response. The antibody isotype produced by sensitized lymphocytes seemed to depend on the immunization schedule. The most effective schedule was one starting early in gestation and comprising frequent administration of antigen. This caused an optimal distribution of sensitized lymphocytes capable of IgA production.

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Section: Productive Capacitysupporting

confidence: 86%

Section: In Ovo Translation Of Mrnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow

Kortbeek-Jacobs

Kooten

Donk

et al. 1984

Veterinary Microbiology

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show abstract

“…The incidence of diarrhea is correlated with lower anti-K88 titers in mammary secretions ( Table 2). Earlier observations already indicated that diarrhea may occur when local production of anti-K88 antibodies in the mammary gland is low (7). However, these findings have to be confirmed by a trial on more animals to establish the full potential of protection of the piglets by oral immunization of the sow.…”

Section: Resultsmentioning

confidence: 80%

Oral immunization of sows: Anti‐K88 antibodies in serum and milk of the sow and in serum of the piglets

Kortbeek-Jacobs¹,

Houten²

1982

Veterinary Quarterly

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mRNA from porcine colostral and milk cells coding for specific antibodies in response to orally administered strains

Cited by 2 publications

References 14 publications

The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow

The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow

Oral immunization of sows: Anti‐K88 antibodies in serum and milk of the sow and in serum of the piglets

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