“…Several electrophysiological, ultrasonographic, Electroneuromyography (ENMG) and histological techniques are also developed and adapted to diagnose PNL (80,81). Subsequently, many other sensitive techniques have been used previously or in development that can be updated and further used as a point of care tools in the laboratory as well as field settings as diagnostic tools such as variants of PCR (8,24,71,72,82), colorimetric assays (83), Biomarkers (66), Filter paper (84), peptide-nucleic-acid-ELISA (85,86), reverse line probe (87), synthetic peptide reagents (88)(89)(90), Ultrasonography (50)(51)(52), Fingerstick test (91), Electrochemical biosensors (53), LAMP (61)(62)(63), MRI (54)(55)(56), LF Assays (92,93), Fluorescent staining (47)(48)(49) and Artificial Intelligence to combat leprosy (44,45). Here we will be discussing budding techniques that have the potential to be used in the field settings easily.…”