“…After completing the reaction, the solution was ultrafiltered with a centrifugal filter device (300 Kd, PALL, Port Washington, NY, USA) to remove excess EDC.HCL and Sulfo-NHS. Then 100 mL of anti-mouse tenascin-C antibodies was added and stirred for 2 h at 37 C. Finally, the conjugated USPIO was purified with phosphate buffered saline (PBS) and stored in PBS (0.1 M, pH 7.4) at 4 C (13,14).…”