In vivo MRI detection of atherosclerosis in ApoE-deficient mice by using tenascin-C-targeted USPIO

Li, Yan; Li, Jun; Song, Jiacheng; Ma, Zhanlong; Shi, Haibin

doi:10.1177/0284185118762613

Cited by 13 publications

(8 citation statements)

References 28 publications

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“…The degree of decline is related to the local iron accumulation (19–21). Here, 24 h after administration was selected as the optimal imaging time, and signal decline is usually seen within 24 h after administration (22,23). The signal decrease was detected at the lesion site.…”

Section: Discussionmentioning

confidence: 99%

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The possibility of visualizing TGF-β1 expression in ApoE^−/− mice atherosclerosis using MR targeted imaging

Xia

Yang

et al. 2023

Acta Radiol

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Background Endothelial TGF-β1 signaling is a primary driver of atherosclerosis-associated vascular inflammation. Targeted imaging and inhibition of the expression of TGF-β1 may reduce the atherosclerotic vessel wall inflammation and stop the progression of atherosclerotic plaque. Purpose To investigate the possibility of the anti-TGF-β1-ultrasmall superparamagnetic iron oxide (USPIO) specific probe as an imaging marker for the expression of TGF-β1 in ApoE–/– mice atherosclerosis detected with 7.0-T magnetic resonance imaging (MRI). Material and Methods Here, 70 ApoE–/– mice on a high-fat diet served as the experimental group and 30 C57BL/6 mice on a normal diet served as the control group. The morphology of plaques was viewed by H&E staining, and the expression and distribution of TNC and TGF-β1 were detected by immunohistochemical staining. Another 40 mice in the experimental group were classified into a targeted group, which was administrated an anti-TGF-β1-USPIO probe, and the pure group, which was injected with pure USPIO. Results The 7.0-T MRI showed that the relative signal intensity (rSI) changes of the targeted group decreased more than those of the pure group (−19.34 ± 0.68% vs. −5.61 ± 0.57%; P < 0.05). Histopathological analyses demonstrated expression of TGF-β1 in atherosclerotic plaque formation progression from 10 to 28 weeks. The MR images of the expression of TGF-β1 in atherosclerosis correlated well with the pathological progression of atherosclerotic plaque formation. Conclusions Anti-TGF-β1-USPIO could provide a useful molecular imaging tool for detecting and monitoring the expression of TGF-β1 in atherosclerotic plaques by MRI.

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Section: Discussionmentioning

confidence: 99%

“…Atherosclerotic plaque formation was dynamically viewed at different times (10,16,22, and 28 weeks). The morphology of aorta plaques was observed by optical microscope (Nikon, Japan) on hematoxylin and eosin (H&E) staining and immunohistochemical staining.…”

Section: Induction Of Atherosclerosis In Apoe-knockout Micementioning

confidence: 99%

The possibility of visualizing TGF-β1 expression in ApoE^−/− mice atherosclerosis using MR targeted imaging

Xia

Yang

et al. 2023

Acta Radiol

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“…A further study demonstrated that tenascin-C, a multifunctional extracellular glycoprotein that is highly expressed in advanced atherosclerotic plaques and is associated with inflammatory changes and plaque rupture, can serve as a marker for atherosclerotic plaques [ 187 ]. After injection of anti-tenascin C-ultra-small IONPs, MR images correlated well with histopathological analysis and the progression of atherosclerotic plaques.…”

Section: Cardiovascular Tissue Regeneration and Engineeringmentioning

confidence: 99%

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

2021

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In recent years, many promising nanotechnological approaches to biomedical research have been developed in order to increase implementation of regenerative medicine and tissue engineering in clinical practice. In the meantime, the use of nanomaterials for the regeneration of diseased or injured tissues is considered advantageous in most areas of medicine. In particular, for the treatment of cardiovascular, osteochondral and neurological defects, but also for the recovery of functions of other organs such as kidney, liver, pancreas, bladder, urethra and for wound healing, nanomaterials are increasingly being developed that serve as scaffolds, mimic the extracellular matrix and promote adhesion or differentiation of cells. This review focuses on the latest developments in regenerative medicine, in which iron oxide nanoparticles (IONPs) play a crucial role for tissue engineering and cell therapy. IONPs are not only enabling the use of non-invasive observation methods to monitor the therapy, but can also accelerate and enhance regeneration, either thanks to their inherent magnetic properties or by functionalization with bioactive or therapeutic compounds, such as drugs, enzymes and growth factors. In addition, the presence of magnetic fields can direct IONP-labeled cells specifically to the site of action or induce cell differentiation into a specific cell type through mechanotransduction.

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“…For instance, intravascular ultrasound and optical coherence tomography [ 10 , 11 , 12 , 13 ] provide information on plaque morphology but are invasive procedures. Other non-invasive imaging strategies might offer new opportunities for atheroma diagnosis [ 14 , 15 ], particularly magnetic resonance imaging (MRI), which combines excellent soft-tissue contrast, good resolution, and absence of exposure to ionizing radiation [ 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

A Nano-Emulsion Platform Functionalized with a Fully Human scFv-Fc Antibody for Atheroma Targeting: Towards a Theranostic Approach to Atherosclerosis

Bonnet

Prévot

Mornet

et al. 2021

IJMS

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Atherosclerosis is at the onset of the cardiovascular diseases that are among the leading causes of death worldwide. Currently, high-risk plaques, also called vulnerable atheromatous plaques, remain often undiagnosed until the occurrence of severe complications, such as stroke or myocardial infarction. Molecular imaging agents that target high-risk atheromatous lesions could greatly improve the diagnosis of atherosclerosis by identifying sites of high disease activity. Moreover, a “theranostic approach” that combines molecular imaging agents (for diagnosis) and therapeutic molecules would be of great value for the local management of atheromatous plaques. The aim of this study was to develop and characterize an innovative theranostic tool for atherosclerosis. We engineered oil-in-water nano-emulsions (NEs) loaded with superparamagnetic iron oxide (SPIO) nanoparticles for magnetic resonance imaging (MRI) purposes. Dynamic MRI showed that NE-SPIO nanoparticles decorated with a polyethylene glycol (PEG) layer reduced their liver uptake and extended their half-life. Next, the NE-SPIO-PEG formulation was functionalized with a fully human scFv-Fc antibody (P3) recognizing galectin 3, an atherosclerosis biomarker. The P3-functionalized formulation targeted atheromatous plaques, as demonstrated in an immunohistochemistry analyses of mouse aorta and human artery sections and in an Apoe−/− mouse model of atherosclerosis. Moreover, the formulation was loaded with SPIO nanoparticles and/or alpha-tocopherol to be used as a theranostic tool for atherosclerosis imaging (SPIO) and for delivery of drugs that reduce oxidation (here, alpha-tocopherol) in atheromatous plaques. This study paves the way to non-invasive targeted imaging of atherosclerosis and synergistic therapeutic applications.

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In vivo MRI detection of atherosclerosis in ApoE-deficient mice by using tenascin-C-targeted USPIO

Abstract: Tenascin-C expression increased with the progression of atherosclerosis. Anti-tenascin-C-USPIO could provide a useful molecular imaging tool for detecting and monitoring atherosclerotic plaques by MRI.

Cited by 13 publications

References 28 publications

The possibility of visualizing TGF-β1 expression in ApoE^−/− mice atherosclerosis using MR targeted imaging

The possibility of visualizing TGF-β1 expression in ApoE^−/− mice atherosclerosis using MR targeted imaging

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

A Nano-Emulsion Platform Functionalized with a Fully Human scFv-Fc Antibody for Atheroma Targeting: Towards a Theranostic Approach to Atherosclerosis

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In vivo MRI detection of atherosclerosis in ApoE-deficient mice by using tenascin-C-targeted USPIO

Abstract: Tenascin-C expression increased with the progression of atherosclerosis. Anti-tenascin-C-USPIO could provide a useful molecular imaging tool for detecting and monitoring atherosclerotic plaques by MRI.

Cited by 13 publications

References 28 publications

The possibility of visualizing TGF-β1 expression in ApoE−/− mice atherosclerosis using MR targeted imaging

The possibility of visualizing TGF-β1 expression in ApoE−/− mice atherosclerosis using MR targeted imaging

Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering

A Nano-Emulsion Platform Functionalized with a Fully Human scFv-Fc Antibody for Atheroma Targeting: Towards a Theranostic Approach to Atherosclerosis

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Product

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The possibility of visualizing TGF-β1 expression in ApoE^−/− mice atherosclerosis using MR targeted imaging

The possibility of visualizing TGF-β1 expression in ApoE^−/− mice atherosclerosis using MR targeted imaging