MPF Governs the Assembly and Contraction of Actomyosin Rings by Activating RhoA and MAPK during Chemical-Induced Cytokinesis of Goat Oocytes

Wu, Yan-Guang; Zhou, Ping; Lan, Guo-Cheng; Gao, Da; Li, Qing; Wei, De-Li; Wang, Huili; Tan, Jing-He

doi:10.1371/journal.pone.0012706

Cited by 10 publications

(14 citation statements)

References 79 publications

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“…In somatic cells, physical and psychological stressors causes oxidative damage by inducing an imbalance between the prooxidant and antioxidant status [67,68] and oxidative stressinduced proteolysis of the anaphase inhibitor securin and mitotic cyclins [62]. Furthermore, studies in rodent [69][70][71] and goat [72,73] oocytes suggest that maturation-promoting factor (MPF) might be an upstream regulator of the MAPK/ MOS pathway during meiotic maturation. It is thus suggested that maternal restraint induces intraoocyte oxidative stress, which then reduces MAPK activities (Fig.…”

Section: Stress and Oocyte Aneuploidymentioning

confidence: 99%

Maternal-Restraint Stress Increases Oocyte Aneuploidy by Impairing Metaphase I Spindle Assembly and Reducing Spindle Assembly Checkpoint Proteins in Mice1

Zhou

Lian

Cui

et al. 2012

Biology of Reproduction

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Studies in both humans and animals suggest detrimental effects of psychological stress on reproduction. Although our recent study shows that maternal-restraint stress diminishes oocyte developmental potential, the mechanism behind this effect is unknown. This prompted us to study the potential role of maternal-restraint stress in the genesis of aneuploidy during meiosis I. At 24 h after equine chorionic gonadotropin injection, mice were subjected to restraint stress for 24 h. After the restraint, some mice were killed to recover immature oocytes for in vitro maturation, while others were injected with human chorionic gonadotropin to recover in vivo matured oocytes. Analysis on chromosome complements of both mature oocytes and parthenotes confirmed that maternal restraint increased aneuploidy in both in vivo and in vitro matured oocytes and that the percentage of aneuploid oocytes were three times higher in the earlier matured oocytes than in the later matured ones. Further observations indicated that maternal restraint 1) impaired metaphase I (MI) spindle assembly while inhibiting MAPK activities, 2) accelerated progression of anaphase I while down-regulating the expression of spindle assembly checkpoint (SAC) proteins, and 3) induced intraoocyte oxidative stress. The following possible model was proposed to explain the results. Maternal-restraint stress increased oocyte aneuploidy by impairing MI spindle assembly and decreasing the SAC. Whereas abnormal spindles would affect centromere attachments, a reduction in SAC would accelerate the anaphase I progression. Failure of centromere attachment, together with the hastened anaphase, would result in nondisjunction of the unattached chromosomes. Furthermore, maternal-restraint stress might also impair spindle assembly and SAC function by inducing intraoocyte oxidative stress, which would then reduce MAPK activity, a critical regulator of microtubule assembly and the establishment and maintenance of the SAC during oocyte maturation.

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Section: Stress and Oocyte Aneuploidymentioning

confidence: 99%

Maternal-Restraint Stress Increases Oocyte Aneuploidy by Impairing Metaphase I Spindle Assembly and Reducing Spindle Assembly Checkpoint Proteins in Mice1

Zhou

Lian

Cui

et al. 2012

Biology of Reproduction

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“…Our previous study using goat oocytes indicated that demecolcine induced ooplasmic protrusion by increasing maturation promoting factor (MPF) and mitogen activated protein kinase (MAPK) activities via activating MAD2 (Wu et al, 2010). Both caffeine and MG132 are MPF-activating agents; whereas MG132, a specific inhibitor of proteasome catalytic activity, activates MPF by preventing cyclin B degradation (Glotzer et al, 1991;Josefsberg et al, 2000), caffeine increases MPF activity by inhibiting Myt1/Wee1 and dephosphorylating CDC2A (Kikuchi et al, 2000;Smythe and Newport, 1992).…”

Section: Introductionmentioning

confidence: 99%

Caffeine Can Be Used for Oocyte Enucleation

Wang

Chang²,

Li³

et al. 2011

Cellular Reprogramming

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The removal of chromosomes from recipient oocytes is one of the key steps in nuclear transfer cloning. Although microtubule interrupters have been successfully used for oocyte enucleation, their potential side effect on oocyte developmental potential should be considered, and less harmful drugs should be explored for chemical-assisted enucleation. Based on our previous findings that any maturation promoting factor-activating agent induces ooplasmic protrusion without disrupting microtubules, we have studied the feasibility to use caffeine or MG132 for chemical-assisted enucleation. Experiments using goat oocytes showed that treatments for 30 min with 1-mM caffeine or 5-μM MG132-induced ooplasmic protrusions in about 85% of the oocytes, a percentage similar to that achieved with optimal demecolcine treatment. Rates of enucleation, cell fusion and in vitro blastulation were similar among caffeine, MG132, and demecolcine enucleation but significantly higher than blind aspiration. Furthermore, neither rates of pregnancy on days 90 and 120 nor the general rate of live births/embryos transferred differed significantly (p > 0.05) between caffeine and demecolcine enucleation. Although oocytes treated with caffeine did not retract protrusions until 2 h, many oocytes treated with MG132 withdrew protrusions as early as 0.5 h after treatment. The optimal treatment to induce ooplasmic protrusion in 75% pig oocytes was 8-mM caffeine for 60 min. Mouse oocytes responded poorly to demecolcine or caffeine with less than 40% forming inconspicuous protrusions following optimal treatments. It is concluded that caffeine can be used for enucleation of goat and pig oocytes with similar results as demecolcine, and live kids were born after caffeine-assisted enucleation.

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“…To assess whether adiponectin acts on oocyte maturation through the MEK 1/2 MAPK pathway, COCs ( n = 430 oocytes) were randomly distributed in groups of 20–25 for one of the following treatments: IVM with 0 or 5 μg/ml adiponectin, in the presence or absence of a specific inhibitor of MEK 1/2 (10 μM; U0126, EMD Millipore Corp., Billerica, MA, USA). The U0126 is a highly selective inhibitor of MEK 1/2 activation and the MAPK cascade in granulosa cells and goat oocytes (Coyral‐Castel, Ramé, Fatet, & Dupont, ; Wu et al, ). The nuclear meiotic stage was determined after 27 hr of IVM.…”

Section: Methodsmentioning

confidence: 99%

Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

Gomes

Costa

Silva

et al. 2018

Reprod Domestic Animals

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Recent studies have shown that adiponectin, an adipokine predominantly produced by adipose tissue, regulates several reproductive processes. However, the mechanisms of action of adiponectin on the maturation of goat oocytes remain to be determined. The aim of this study was to investigate whether (a) adiponectin influences the meiotic maturation of goat oocytes; (b) MAPK MEK 1/2 mediates the effects of adiponectin; and 3) adiponectin differentially affects mRNA relative abundance of genes relevant for adiponectin signal transduction in goat oocytes. The addition of adiponectin (5 μg/ml) during the maturation of goat oocytes resulted in a higher percentage of successful nuclear maturation compared to those of the group without adiponectin (p < 0.05). Adiponectin-stimulated nuclear oocyte maturation was significantly impaired by a mitogen-activated protein kinase MEK 1/2 inhibitor, U0126 (p < 0.05). There was no evidence of any adiponectin-induced difference in the relative transcript abundances of AdipoR1, AdipoR2, AMPKα1, AMPKα2, PPARα and PPARγ genes. In conclusion, these results indicate that adiponectin has a positive effect on the meiotic maturation of goat oocytes through the MAPK MEK 1/2 pathway. Furthermore, the adiponectin does not affect the relative abundance of genes relevant for adiponectin signal transduction in goat oocytes.

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MPF Governs the Assembly and Contraction of Actomyosin Rings by Activating RhoA and MAPK during Chemical-Induced Cytokinesis of Goat Oocytes

Cited by 10 publications

References 79 publications

Maternal-Restraint Stress Increases Oocyte Aneuploidy by Impairing Metaphase I Spindle Assembly and Reducing Spindle Assembly Checkpoint Proteins in Mice1

Maternal-Restraint Stress Increases Oocyte Aneuploidy by Impairing Metaphase I Spindle Assembly and Reducing Spindle Assembly Checkpoint Proteins in Mice1

Caffeine Can Be Used for Oocyte Enucleation

Effects of adiponectin during in vitro maturation of goat oocytes: MEK 1/2 pathway and gene expression pattern

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