MP07-17 STONE ABLATION EFFICACY OF A NEW PROTOTYPE HOLMIUM:YAG PULSE-MODULATED LASER AT WORKING DISTANCES OF UP TO 3 mm

Abstract

investigate the role of Fabp4 on urolithiasis. 2)An in vitro study using murine renal tubular cells (RTC) was conducted to examine calcium oxalate crystallization via Fabp4. We compared the control group with the Fabp4 knockdown group after 6 hours exposure of calcium oxalate monohydrate (COM).RESULTS: 1)The Fabp4 KO mice had significantly larger amounts of renal crystal deposits and urinary crystal than the WT mice. Additionally, the gene expression level of Ccl2 was lower in the Fabp4 KO mice than in the WT mice (figure 1A). The RNA-sequence result demonstrated that there were 16 genes commonly differentiated (>2fold) before and after renal crystal development in the Fabp4 KO mice compared with the WT mice. These genes were associated with downregulation of antioxidant activity, IgA binding, and Toll-like receptor binding; cell-cell adhesion and neutrophil aggregation; and IgA immunoglobulin complex in Gene Ontology analysis.2)In the Fabp4 knockdown RTC, COM crystal endocytosis rate was significantly lower than the control. Gene expression levels of Ccl2 and Tumor necrosis factor were higher in the Fabp4 knockdown RTC than those of the control (figure 1B).CONCLUSIONS: We have found that Fabp4 deficiency suppresses macrophage function and crystal endocytosis in renal tubular stones, leading to stone development. These data support a causal role for FABP4 in driving urinary stone formation.

Lasers for stone lithotripsy: advantages/disadvantages of each laser source

“…As for the future, the possibility to apply artificial intelligence could decease lesions to surrounding tissues keeping adequate speed and precision [33]. Also Ho:YAG with micro-pulse packets have provided significantly greater stone ablation in comparison to thulium laser fiber (TFL) [34].…”

Which Is the Best Laser for Lithotripsy? Holmium Laser

“…Holmium lasers with micro-pulse packets are the next step. A prototype holmium system with these features provided significantly greater stone ablation in comparison to TFL [10] .…”