Mouse paw anaphylaxis

Effect of PAF‐acether antagonists on active anaphylactic mouse paw edema

“…The present results show that the glucocorticoid prednisolone suppresses the inflammatory response, whereas dexamethasone at doses that inhibit carrageenin-induced paw edema (0.25 and 0.5 mg/kg) (16) failed to interfere with the reaction and required doses as high as 1.9 mg/kg to be effective. Similar results were reported by Kaneta et al (17) who showed that dexamethasone does not protect mice from passive paw anaphylaxis at doses of 0.1 mg/kg administered once a day for two days. On the basis of the model used by our group (edema by antigen versus edema by carrageenin), the different dose requirements for glucocorticosteroids confirm that these anti-inflammatory agents interfere with multiple targets and/or the synthesis of inhibitory proteins (18).…”

Mouse IgE-Mediated Paw Anaphylaxis in Mice and Rats

“…In addition, PPA was also demonstrated with native antiserum after a shor ter (2 h) or a longer (6 days) sensitization period (data not shown). The time course and peak height of this 2-hour PPA in mice were almost identical to those of the mouse IgGl-mediated reaction described previ ously [3]. Interestingly, the 2-hour PPA in mice had the peak invariably at 15 min after antigen challenge; the difference in peak time between the 2-hour and 72-hour PPA was estimated to be statistically signifi cant at the 1 % level by a rank test [14].…”

“…Release of biologically active constituents from the mast cells of the skin appears to be the underlying mechanism of increased vascular permeability in PPA [3] as well as in PCA [10]. In PPA reactions, massive serous exudation is distinctive, and measuring paw thickness is easier and more accurate than any proce dures for the quantitation of PCA [4,8,12,15].…”

“…We also reported the induction of mouse PPA with IgGl, another class of mouse homocyto tropic antibodies [3].…”

“…In rats, the injecting volume of each solution was doubled. Before and af ter antigen challenge, paw thickness was measured us ing a dial thickness gauge (Ozaki Manufacturing Co., Tokyo, Japan) [3]. Figure 1 shows that maximum swelling was appar ent 10 min in mice and 15 min in rats after antigen challenge and that the heated antiserum and the anti mouse IgE-treated antiserum, both of which were the same as those used in the above PCA tests, were nearly completely devoid of the 72-hour PPA-eliciting activities in mice and rats.…”