Mouse model of haemorrhagic septicaemia: dissemination and multiplication of Pasteurella multocida B:2 in vital organs after intranasal and subcutaneous challenge in mice

Kharb, Subhash; Charan, Shiv

doi:10.1007/s11259-012-9547-5

Cited by 17 publications

(16 citation statements)

References 15 publications

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“…Recently, a mouse model of HS caused by P. multocida serotype B:2 has been developed (222), which might enable further exploration of the roles of different host immune cells and host factors as well as bacterial factors in dissemination of the bacteria in the host.…”

Section: Pasteurellosis and Hemorrhagic Septicemiamentioning

confidence: 99%

Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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SUMMARY In a world where most emerging and reemerging infectious diseases are zoonotic in nature and our contacts with both domestic and wild animals abound, there is growing awareness of the potential for human acquisition of animal diseases. Like other Pasteurellaceae , Pasteurella species are highly prevalent among animal populations, where they are often found as part of the normal microbiota of the oral, nasopharyngeal, and upper respiratory tracts. Many Pasteurella species are opportunistic pathogens that can cause endemic disease and are associated increasingly with epizootic outbreaks. Zoonotic transmission to humans usually occurs through animal bites or contact with nasal secretions, with P. multocida being the most prevalent isolate observed in human infections. Here we review recent comparative genomics and molecular pathogenesis studies that have advanced our understanding of the multiple virulence mechanisms employed by Pasteurella species to establish acute and chronic infections. We also summarize efforts being explored to enhance our ability to rapidly and accurately identify and distinguish among clinical isolates and to control pasteurellosis by improved development of new vaccines and treatment regimens.

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Section: Pasteurellosis and Hemorrhagic Septicemiamentioning

confidence: 99%

Pasteurella multocida: from Zoonosis to Cellular Microbiology

2013

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“…Earlier, Abdullah et al (2013b) had reported the successful recovery of P. multocida using PCR from various organs of mice inoculated with contaminated river water. HS is a deadly infection and the extent of lesion manifestation depends on the duration of the disease and the dose of bacterial inoculums (Kharb and Charan, 2013). This is in line with the findings of this study, where moderate and severe lesions were observed following inoculation with contaminated water that had stayed for 72 h in comparison to lesions observed following inoculation with contaminated water that had stayed for 24 and 48 h. This shows that the P. multocida was more active at 72 h post contamination, perhaps due to decomposition of the mice carcass and shedding of the bacteria into the water.…”

Section: Discussionmentioning

confidence: 99%

HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY <i>PASTEURELLA MULTOCIDA </i>TYPE B:2

Khaleel

Abdullah

Adamu

et al. 2014

American Journal of Animal and Veterinary Sciences

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Haemorrhagic Septicaemia (HS) is a fatal systemic disease of bovines typified by an acute, highly fatal septicaemic disease with high morbidity and mortality. In this study, sixty five adult ICR mice were used for the study. In the first phase, 5 mice each were inoculated with 10 9 cfu of P. multocida type B:2 through the intraperitoneal route. After development of classical signs of HS, the mice were euthanised and dropped into a tank of river waterfor 24, 48 and 72 h. Contaminated water from the tank were collected at the three different times (24, 48 and 72 h) and was used for intraperitoneal, oral and aerosol inoculation of 15 mice each with a dose of 1 mL −1 10 9 cfu (intraperitoneal and aerosol) and 0.4 mL −1 10 9 cfu (oral). The control group (n = 5) was given phosphate buffered saline. All mice in the three groups and control were culled after 72 h post inoculation and the vital organs (liver, spleen, lung, kidney, heart, brain) were collected in 10% buffered formalin, procesed, sectioned and stained with H&E. Degeneration and necrosis, hemorrhage and infiltration of inflammatory cells were the most observed lesions in all the organs, while thrombosis and oedema were least observed. The lesion severity was moderate to severe using contaminated water at 72 h post contamination and mild to moderate when inoculated with water 24 and 48 h post contamination. Mice inoculated through the oral and intraperitoneal routes recorded more severe lesion compared with those inoculated through the aerosol route. However, using contaminated water at 24 h, mice inoculated through the aerosol route had the highest distribution of inflammatory cells in the lungs, which declined following inoculation with contaminated water at 48 and 72 h, respectively.This study has shown that contamination of water bodiesby carcasses of HS infected animals is a likely source of infection to heathy animals in the wild and that development of lesions severity is time dependant and significantly higher using contaminated water at 72 h than water contaminated for 24 and 48 h. This further indicates the danger of leaving decomposing carcasses in water bodies for prolonged periods.

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“…The organism is the etiological agent of Haemorrhagic septicaemia in cattle and water buffalo and it is predominant in South and South East Asia and to most parts of tropical Africa (Abubakar and Zamri-Saad, 2011;Kharb and Charan, 2013;Khaleel et al, 2013;Abdullah et al, 2013a;Jesse et al, 2013a;. HS is a peracute disease and is considered to be one of the most economically important diseases in Asia particularly in South and South East Asia leading to huge economic loss in livestock industry (Jesse et al, 2013a).…”

Section: Intoductionmentioning

confidence: 99%

“…There are several disease models of infection with P. multocida using mice and show the suitability of mice to study the pathogenesis of P. multocida to produce HS with same symptoms and lesion in cattle and water buffalo (Affandi et al, 2012;Jamal et al, 2013;Faez et al, 2013). In pure chemical form endotoxin is a Lipopolysaccharide (LPS) which has widely variable composition (or chemotype) and exerts a wide range of often opposing influences on host pathogen interactions (Khaleel et al, 2013;Kharb et al, 2013). The key virulence factors identified in Pasteurella multocida type B: 2 include capsule, lipopolysaccharides, surface adhesions, ironregulated and iron acquisition proteins (Affandi et al, 2012;Kharb et al, 2013).…”

Section: Intoductionmentioning

confidence: 99%

“…In pure chemical form endotoxin is a Lipopolysaccharide (LPS) which has widely variable composition (or chemotype) and exerts a wide range of often opposing influences on host pathogen interactions (Khaleel et al, 2013;Kharb et al, 2013). The key virulence factors identified in Pasteurella multocida type B: 2 include capsule, lipopolysaccharides, surface adhesions, ironregulated and iron acquisition proteins (Affandi et al, 2012;Kharb et al, 2013). The protein toxin from Pasteurella multocidatype B: 2 experimentally induce all of the major symptoms of a number of economically important diseases in domestic livestock (Jamal et al, 2013;Ataei et al, 2009;Faez et al, 2013).…”

Section: Intoductionmentioning

confidence: 99%

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Alterations in Interleukin-1Î² and Interleukin-6 in Mice Inoculated Through the Oral Routes Using Graded Doses of <i>p. Multocida</i> Type b: 2 and its Lipopolysaccharide

Ali¹,

Adamu²,

Abdullah³

et al. 2015

American Journal of Animal and Veterinary Sciences

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The gram-negative bacterium Pasteurella multocida serotype B: 2 is an important veterinary and opportunistic human pathogen that causes deplorable welfare problems and colossal economic production losses in bovine and other animal species throughout the world. Pasteurella multocida type B: 2 and its LPS are associated with Haemorrhagic Septicaemia (HS) in mice as animal model. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n = 10) and these were inoculated with 0.4 mL of PBS pH 7.4 orally. The treatment groups (B; n = 25 and C; n = 25) were inoculated with P. multocida type B: 2 and lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B10 1 , B10 3 , B10 5 , B10 7 and B10 9 for Pasteurella multocida and C10 1 , C10 3 , C10 5 , C10 7 and C10 9 for LPS respectively. The mice were observed for 120 h post-inoculation. The concentration of IL-1β was significantly higher (p<0.001) in the B10 7 of P. multocida type B: 2 and C10 9 of LPS compared to the control group and the other treatment groups. The concentration of IL-6 was significantly higher (p<0.001) in the B10 3 cfu of P. multocida type B: 2 and C10 9 cfu of LPS compared to the control group and the other treatment groups. Interleukin-1β and interleukin-6 evaluated in this study were predominantly associated with HS in varying doses of 10 1 , 10 3 , 10 5 , 10 7 and 10 9 in the whole cell and its LPS respectively. Therefore, the target of this study was to investigate on proinflammatory response in mice infected with graded doses of Pasteurella multocida serotype B: 2 and its LPS via the oral route of inoculation.

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Mouse model of haemorrhagic septicaemia: dissemination and multiplication of Pasteurella multocida B:2 in vital organs after intranasal and subcutaneous challenge in mice

Cited by 17 publications

References 15 publications

Pasteurella multocida: from Zoonosis to Cellular Microbiology

Pasteurella multocida: from Zoonosis to Cellular Microbiology

HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY <i>PASTEURELLA MULTOCIDA </i>TYPE B:2

Alterations in Interleukin-1Î² and Interleukin-6 in Mice Inoculated Through the Oral Routes Using Graded Doses of <i>p. Multocida</i> Type b: 2 and its Lipopolysaccharide

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