Mouse Model of Diethylnitrosamine-Induced Gastric Cancer

Binato, Marcelo; Schmidt, M; Volkweis, Bernardo Silveira; Ribeiro, Guilherme Behrend Silva; Edelweiss, Maria Isabel Albano; Gurski, Richard Ricachenevsky

doi:10.1016/j.jss.2007.12.748

Cited by 17 publications

(10 citation statements)

References 26 publications

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“…151,152 DEN is frequently used as a carcinogenic agent; 152 however, the target organ in which it induces malignant tumors is species specific. Thus, mice treated with DEN develop liver tumors as well as gastrointestinal, 153 skin, lung, 154 and haematopoietic malignancies. 155 In addition, the time needed to develop HCC after a single DEN injection depends on the administered dose, and the gender, age, and strain of the treated mice.…”

Section: Animal Models Of Liver Cancermentioning

confidence: 99%

MicroRNAs in liver cancer: a model for investigating pathogenesis and novel therapeutic approaches

et al. 2014

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MicroRNAs (miRNAs) constitute a large class of short RNAs (e.g., 20-24 nucleotides in length), whose main function is to posttranscriptionally regulate the expression of protein-coding genes. Their importance in tumorigenesis has been demonstrated over the past decade, and correspondingly, they have emerged as potential therapeutic molecules and targets. Liver cancer is one of the most common neoplastic diseases worldwide, and it currently has a poor prognosis owing to largely ineffective therapeutic options. Liver cancer is also an excellent model for testing miRNA-based therapy approaches as it can be easily targeted with the systemic delivery of oligonucleotides. In recent years, the role of miRNAs in hepatocellular carcinoma (HCC) has been established with molecular studies and the development of animal models. These studies have also provided the basis for evaluating the therapeutic potential of miRNAs, or anti-miRNAs. In general, the safety of miRNAs has been proven and antitumor activity has been observed. Moreover, because of the absence or presence of mild side effects, the prophylactic use of miRNA-based approaches may be foreseen.

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Section: Animal Models Of Liver Cancermentioning

confidence: 99%

MicroRNAs in liver cancer: a model for investigating pathogenesis and novel therapeutic approaches

et al. 2014

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“…The target organ in which DEN induces malignant tumours is species specific. Mice mainly develop not only liver tumours, but also gastrointestinal (Binato et al. 2008), skin, respiratory (Wang et al.…”

Section: Chemically Induced Modelsmentioning

confidence: 99%

“…The target organ in which DEN induces malignant tumours is species specific. Mice mainly develop not only liver tumours, but also gastrointestinal (Binato et al 2008), skin, respiratory (Wang et al 1992) and haematopoietic tumours (Gray et al 1991). The carcinogenic capacity of DEN is situated in its capability of alkylating DNA structures.…”

Section: Chemically Induced Modelsmentioning

confidence: 99%

Experimental mouse models for hepatocellular carcinoma research

Heindryckx

Colle

Vlierberghe

2009

Int J Experimental Path

311

331

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Every year almost 500,000 new patients are diagnosed with hepatocellular carcinoma (HCC), a primary malignancy of the liver that is associated with a poor prognosis. Numerous experimental models have been developed to define the pathogenesis of HCC and to test novel drug candidates. This review analyses several mouse models useful for HCC research and points out their advantages and weaknesses. Chemically induced HCC mice models mimic the injury-fibrosis-malignancy cycle by administration of a genotoxic compound alone or, if necessary, followed by a promoting agent. Xenograft models develop HCC by implanting hepatoma cell lines in mice, either ectopically or orthotopically; these models are suitable for drug screening, although extrapolation should be considered with caution as multiple cell lines must always be used. The hollow fibre assay offers a solution for limiting the number of test animals in xenograft research because of the ability for implanting multiple cell lines in one mouse. There is also a broad range of genetically modified mice engineered to investigate the pathophysiology of HCC. Transgenic mice expressing viral genes, oncogenes and/or growth factors allow the identification of pathways involved in hepatocarcinogenesis

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“…This model of HCC is developed by administering N-nitrosodiethylamine (DEN) to mice [9,10]. The carcinogenetic activity of DEN is exerted in two different ways: (i) by alkylating DNA structures thus causing DNA damage and subsequent cell degeneration and (ii) by inducing reactive oxygen species (ROS) formation through the activation of the cytochrome P450 in hepatocytes [11,12].…”

Section: Experimental Models Of Hccmentioning

confidence: 99%

Liver carcinogenesis: Rodent models of hepatocarcinoma and cholangiocarcinoma

Minicis¹,

Kisseleva²,

Francis³

et al. 2013

Digestive and Liver Disease

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Hepatocellular carcinoma and cholangiocarcinoma are primary liver cancers, both represent a growing challenge for clinicians due to their increasing morbidity and mortality. In the last few years a number of in vivo models of hepatocellular carcinoma and cholangiocarcinoma have been developed. The study of these models is providing a significant contribution in unveiling the pathophysiology of primary liver malignancies. They are also fundamental tools to evaluate newly designed molecules to be tested as new potential therapeutic agents in a pre-clinical set. Technical aspects of each model are critical steps, and they should always be considered in order to appropriately interpret the findings of a study or its planning. The purpose of this review is to describe the technical and experimental features of the most significant rodent models, highlighting similarities or differences between the corresponding human diseases. The first part is dedicated to the discussion of models of hepatocellular carcinoma, developed using toxic agents, or through dietary or genetic manipulations. In the second we will address models of cholangiocarcinoma developed in rats or mice by toxin administration, genetic manipulation and/or bile duct incannulation or surgery. Xenograft or syngenic models are also proposed.

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Mouse Model of Diethylnitrosamine-Induced Gastric Cancer

Cited by 17 publications

References 26 publications

MicroRNAs in liver cancer: a model for investigating pathogenesis and novel therapeutic approaches

MicroRNAs in liver cancer: a model for investigating pathogenesis and novel therapeutic approaches

Experimental mouse models for hepatocellular carcinoma research

Liver carcinogenesis: Rodent models of hepatocarcinoma and cholangiocarcinoma

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