Motor-like microlasers functioning in biological fluids

Wang, Ziyihui; Shawn, Lingwei; Gao, Zehang; Chan, Kok Ken; Gong, Chen; Wang, Chenlu; Xu, Tianhua; Liu, Tiegen; Feng, Shilun; Chen, Yu‐Cheng

doi:10.1039/d2lc00513a

Cited by 5 publications

(2 citation statements)

References 43 publications

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“…Recently reported LC sensor based on the whispering gallery mode (WGM) due to the simple structure, high quality factor, and small mode volume of WGM optical microcavities have been widely used in a variety of fields. [15][16][17][18] On the basis of naked eye observation utilising POM, the optofluidic sensor is implemented by integrating the POM with the WGM laser spectroscopy, resulting in a more sensitive and precise characterisation. [19][20][21][22][23] In this work, we presented a biosensing platform based on the LC-amplified optofluidic WGM resonator, as shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

Detection of streptavidin using liquid crystal based whispering gallery mode microbubble

Wang

Xu²,

Wang³

et al. 2022

Advanced Sensor Systems and Applications XII

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Protein is a complex chemical substance essential for human survival. Traditional protein detection methods, such as colorimetry, electrochemical analysis, and enzyme-linked immunosorbent assays, have shown good specificity and accuracy for the protein detection. However, all these methods require specialized instruments, and the detection procedures are laborious and time-consuming. As a result, a rapid, sensitive, label-free protein detection method is urgently needed. Herein, we have developed an ultra-sensitive biosensor for the detection of low-concentration protein molecules, employing liquid crystal (LC)-amplified optofluidic resonator. Since the orientations of LCs highly depend on the surface biomolecular binding processes, LCs can be employed to realize the extremely sensitive detection of biomolecules. Immobilized protein molecules interfere with the orientation of LCs by reducing the vertical anchoring force from the alignment layer in which the spectral wavelength shift was monitored as a sensing parameter. A biosensing platform based on an LC-amplified optofluidic whispering gallery mode (WGM) resonator was designed and studied accordingly. Due to the simultaneous interaction of the WGM and the LCs in the optofluidic resonator, the changes caused by the injection of protein molecules will be amplified, resulting in a shift in the resonance wavelength. Total wavelength shifts scale proportionally to the molecular concentrations of the protein within a certain range. The detection limit for streptavidin (SA) can reach as low as the femtometer level, which is significantly higher than the detection limit in the classic polarized optical microscope (POM) method visible with the naked eye. In addition to SA, the LC-based optofluidic resonator can also be applied to detect a variety of protein molecules. Our study demonstrates that LC-amplified optofluidic resonator can provide a novel solution for ultrasensitive real-time characterization of biosensing and biomolecular interactions.

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Section: Introductionmentioning

confidence: 99%

Detection of streptavidin using liquid crystal based whispering gallery mode microbubble

Wang

Xu²,

Wang³

et al. 2022

Advanced Sensor Systems and Applications XII

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“…To address this issue, micro/nanomotors have emerged as active agents for biosensing owing to their unique ability to propel and travel in biofluids. By converting energy from the surrounding environment into autonomous movement, self-propelled motor-based biosensors have gained considerable attention. − Studies have demonstrated that the continuous movement of micromotors can enhance target binding efficiency and sensitivity, allowing direct identification and isolation of different biological targets. − The significantly improved analyte–receptor interactions can also be obtained through the increased fluid transport associated with propelled motions of micromotors. Through the integration of light-emitting fluorophores, self-propelled motors can continuously monitor and reflect the status of an inhomogeneous biological microenvironment.…”

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confidence: 99%

Autonomous Microlasers for Profiling Extracellular Vesicles from Cancer Spheroids

et al. 2023

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Self-propelled micro/nanomotors are emergent intelligent sensors for analyzing extracellular biomarkers in circulating biological fluids. Conventional luminescent motors are often masked by a highly dynamic and scattered environment, creating challenges to characterize biomarkers or subtle binding dynamics. Here we introduce a strategy to amplify subtle signals by coupling strong light–matter interactions on micromotors. A smart whispering-gallery-mode microlaser that can self-propel and analyze extracellular biomarkers is demonstrated through a liquid crystal microdroplet. Lasing spectral responses induced by cavity energy transfer were employed to reflect the abundance of protein biomarkers, generating exclusive molecular labels for cellular profiling of exosomes derived from 3D multicellular cancer spheroids. Finally, a microfluidic biosystem with different tumor-derived exosomes was employed to elaborate its sensing capability in complex environments. The proposed autonomous microlaser exhibits a promising method for both fundamental biological science and applications in drug screening, phenotyping, and organ-on-chip applications.

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Protein aggregation monitoring in microdisk optofluidic sensor through microcavity enhanced Raman scattering

Wang,

Zhang,

et al. 2023

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Motor-like microlasers functioning in biological fluids

Abstract: Microlasers integrated with biological systems have received tremendous attention for their intense light intensity and narrow linewidth recently, serving as a powerful tool for studying complex dynamics and interactions in...

Cited by 5 publications

References 43 publications

Detection of streptavidin using liquid crystal based whispering gallery mode microbubble

Detection of streptavidin using liquid crystal based whispering gallery mode microbubble

Autonomous Microlasers for Profiling Extracellular Vesicles from Cancer Spheroids

Protein aggregation monitoring in microdisk optofluidic sensor through microcavity enhanced Raman scattering

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