Motion of cancer-cell lamellipodia perturbed by laser light of two wavelengths

Xiao, Jian-Long; Hsu, Tsi-Hsuan; Hsu, Ping-Yu; Yang, Weijie; Kuo, P. C.; Lee, Chau‐Hwang

doi:10.1063/1.3517448

Cited by 7 publications

(7 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biener et al found that the polarization of light could apply torques on the actin filaments in cells and hence influenced the motility of neuroblastoma cells, which tended to align along the direction of polarization of the electric field [5]. Using a two-wavelength setup, Xiao et al verified that focused laser light spots can be used to influence the growth and retraction of cellular lamellipodia [6]. Recently, spatial light modulators (SLMs) have been employed to facilitate a variety of cellular guidance.…”

Section: Introductionmentioning

confidence: 99%

Directional migration of cancer cells induced by a blue light intensity gradient

Lan¹,

Lu²,

Pan

et al. 2015

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Abstract:We used a spatial light modulator to project an optical micropattern of 473 nm light with a quartic intensity gradient on a single lung cancer cell. We observed that the intracellular amounts of reactive oxygen species (ROS) of the cancer cells were proportional to the intensity of the blue light, and the blue light intensity gradients could drive directional cell migration. This optically induced directional cell migration was inhibited by a ROS scavenger in the culture medium in a dosedependent manner. In contrast, the ROS levels in fibroblasts were saturated by the blue light at low intensity and therefore the fibroblasts did not exhibit directional migration in the intensity gradient.

show abstract

Section: Introductionmentioning

confidence: 99%

Directional migration of cancer cells induced by a blue light intensity gradient

Lan¹,

Lu²,

Pan

et al. 2015

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…This study of cell size, providing a three-dimensional view, was performed using an optimized optical profilometry (Schmidt and Black, 1992; Chen et al, 2009; Xiao et al, 2010; Zouani et al, 2012; Pan et al, 2011; Lei et al, 2013) and a specific cell treatment protocol, which allowed for a vertical nanometer resolution. Other features that are characteristic of a mature osteoblast include (i) an increase in Runx2 and Col-I expression and (ii) an increase in ECM (osteoid-like matrix) production.…”

Section: Introductionmentioning

confidence: 99%

Insights into the osteoblast precursor differentiation towards mature osteoblasts induced by continuous BMP-2 signaling

et al. 2013

View full text Add to dashboard Cite

SummaryMature osteoblasts are the cells responsible for bone formation and are derived from precursor osteoblasts. However, the mechanisms that control this differentiation are poorly understood. In fact, unlike the majority of organs in the body, which are composed of “soft” tissue from which cells can easily be isolated and studied, the “hard” mineralized tissue of bone has made it difficult to study the function of bone cells. Here, we established an in vitro model that mimics this differentiation under physiological conditions. We obtained mature osteoblasts and characterized them on the basis of the following parameters: the strong expression of osteoblastic markers, such as Runx2 and Col-I; the achievement of specific dimensions (the cell volume increases 26-fold compared to the osteoblast precursors); and the production of an abundant extracellular matrix also called osteoid. We demonstrated that the differentiation of osteoblast precursors into mature osteoblasts requires the continuous activation of Bone Morphogenetic Protein (BMP) receptors, which we established with the immobilization of a BMP-2mimetic peptide on a synthetic matrix mimicking in vivo microenvironment. Importantly, we demonstrated that the organization of the F-actin network and acetylated microtubules of the cells were modified during the differentiation process. We showed that the perturbation of the F-actin cytoskeleton organization abolished the differentiation process. In addition, we demonstrated that expression of the Runx2 gene is required for this differentiation. These findings demonstrate the retro-regulation of cytoplasmic and genic components due to the continuous induction of BMP-2 and also provide more detailed insights into the correct signaling of BMPs for cell differentiation in bone tissue.

show abstract

“…This wavelength was selected for the compromise between high optical effects on perturbing the cell movements and low phototoxicity in comparison with the 405 nm light used in our previous work. 10 Because the shape and size of an optical pattern remained the same within a propagation distance longer than 20 μm, the optical patterns actually formed light walls around the adherent cells. Under suitable intensity and contrast of the optical pattern, the cells continued proliferating to fill up the region enclosed by the light walls.…”

Section: Introductionmentioning

confidence: 99%

“…It is usually difficult to vary the culture patterns during experiments. In contrast, using laser light to pattern cell growth, 7,8 to influence the directions of cell migration, 9,10 or to detach and re-orientate adherent cells, 11 is dynamic and accurate in both spatial and temporal domains. In addition, a number of research groups have demonstrated arbitrary patterning of floating cells by using optoelectronic tweezers.…”

Section: Introductionmentioning

confidence: 99%

Optically micropatterned culture of adherent cells

2012

Self Cite

View full text Add to dashboard Cite

We used a liquid-crystal spatial light modulator to project 473 nm light patterns surrounding a region of adherent cells and achieved an arbitrarily micropatterned cell culture. For a group of ∼60 cells, the cell boundaries fit the pattern of light within 15% deviation of the side length. We also demonstrated a wound-healing experiment with a definite starting temporal point by using this technique. While observing mitochondrial structures in the illuminated cells, we found that the 473 nm light damaged the integrity of mitochondria and thus prohibited cell proliferation in the illuminated region.

show abstract

Motion of cancer-cell lamellipodia perturbed by laser light of two wavelengths

Cited by 7 publications

References 13 publications

Directional migration of cancer cells induced by a blue light intensity gradient

Directional migration of cancer cells induced by a blue light intensity gradient

Insights into the osteoblast precursor differentiation towards mature osteoblasts induced by continuous BMP-2 signaling

Optically micropatterned culture of adherent cells

Contact Info

Product

Resources

About