Optically micropatterned culture of adherent cells

Xiao, Jian-Long; Pan, Huei-Jyuan; Lee, Chau‐Hwang

doi:10.1117/1.jbo.17.7.075004

Cited by 8 publications

(7 citation statements)

References 21 publications

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“…The setup used in the present work is the same as that we described in our previous publications [7,8]. In brief, the light source was a 473 nm diode-pumped solid state laser.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, spatial light modulators (SLMs) have been employed to facilitate a variety of cellular guidance. For example, 473 nm blue light patterns generated by a SLM had been used to conduct patterned cell proliferation [7] and guided migration [8] of adherent cells. Apparently, optical illumination is an effective way to control cell migration.…”

Section: Introductionmentioning

confidence: 99%

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Directional migration of cancer cells induced by a blue light intensity gradient

Lan¹,

Lu²,

Pan

et al. 2015

Biomed. Opt. Express

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Abstract:We used a spatial light modulator to project an optical micropattern of 473 nm light with a quartic intensity gradient on a single lung cancer cell. We observed that the intracellular amounts of reactive oxygen species (ROS) of the cancer cells were proportional to the intensity of the blue light, and the blue light intensity gradients could drive directional cell migration. This optically induced directional cell migration was inhibited by a ROS scavenger in the culture medium in a dosedependent manner. In contrast, the ROS levels in fibroblasts were saturated by the blue light at low intensity and therefore the fibroblasts did not exhibit directional migration in the intensity gradient.

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“…The setup used in the present work is the same as that we described in our previous publications [7,8]. In brief, the light source was a 473 nm diode-pumped solid state laser.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Directional migration of cancer cells induced by a blue light intensity gradient

Lan¹,

Lu²,

Pan

et al. 2015

Biomed. Opt. Express

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“…Optical contrast is a direct way of measuring a combined set of biological parameters, including tissue composition (absorption, scattering). Michelson contrast is often used in optical imaging and vision science [21][22][23] along with other several metrics, such as a simple contrast ratio (ratio dark to light) and clinically relevant Weber contrast. 24 Michelson contrast is a method of choice for quantitative contrast evaluation when the contrast of grids and other patterns need to be assessed.…”

Section: Validation Of the Approach: Contrast Measurements With Blankmentioning

confidence: 99%

Multispectral measurement of contrast in tissue-mimicking phantoms in near-infrared spectral range of 650 to 1600 nm

et al. 2014

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In order to identify the optimal imaging conditions for the highest spatial contrast in biological tissue, we explored the properties of a tissue-mimicking phantom as a function of the wavelengths in a broad range of near-infrared spectra (650 to 1600 nm). Our customized multispectral hardware, which featured a scanning transmission microscope and imaging spectrographs equipped with silicon and InGaAs charge-coupled diode array detectors, allowed for direct comparison of the Michelson contrast obtained from a phantom composed of a honeycomb grid, Intralipid, and India ink. The measured contrast depended on the size of the grid, luminance, and the wavelength of measurements. We demonstrated that at low thickness of the phantom, a reasonable contrast of the objects can be achieved at any wavelength between 700 and 1400 nm and between 1500 and 1600 nm. At larger thicknesses, such contrast can be achieved mostly between 1200 and 1350 nm. These results suggest that distinguishing biological features in deep tissue and developing contrast agents for in vivo may benefit from imaging in this spectral range.

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“…It is also simple to couple the laser light into microfluidic devices for precise cell manipulation [4,5]. In our previous study, we had shown that the optical patterns projected on the bottom of a culture dish could enforce micropatterned culture of adherent cells [6]. On the bases of these studies, we believe that guiding cell migration by optical patterns is feasible.…”

Section: Introductionmentioning

confidence: 99%