Motility of spermatozoa from shovelnose sturgeon and paddlefish

Abstract

The spermatozoa in the seminal plasma from shovelnose sturgeon Scuphirhynchi6s pluforynchus and paddlefish Polyodon sputhulu were immotile with only a few spontaneously motile spermatozoa for 5-10 and 10-20 s, respectively. Spermatozoa of shovelnose sturgeon were observed to be 100% motile immediately after sperm dilution in 10mM NaCl and 20mM Tris-HCI, pH 8.5. The duration of mass progressive movement was 2-3 min; and 1 to 5% of spermatozoa remain active after 360 s (P<O.OI). Spermatozoa of paddlefish demonstrated the best motility 10 s after dilution in 10 mM NaCl with 20 mM Tris-HC1, pH 8.5. The duration of mass progressive movement was 2-3 min and 1 to 5% of spermatozoa remained active after 370 s (P<O.OI). The spermatozoa of shovelnose sturgeon and paddlefish were motile in a range of osmotic pressure from 0 to 100 mosmol kg-' and 0 to 120 mosmol kg-', respectively. The best results with short-term storage of sperm from shovelnose sturgeon and paddlefish were observed in 100 mM glucose+20 mM Tris-HCI, pH 8.5 and 150 mM glucose+20 mM Tris-HCI, pH 8.5.(; 1995 The Fisheries Society of the British Isles

Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effects of pH, dilution rate, ions and osmolality

“…Our data suggest that the optimal pH for sperm motility induction and prolongation is pH 8.0. According to the results, it is confirmed again that the alkaline conditions of diluents enhance the motility parameters of sperm in A. persicus, similar to other sturgeon species such as Polyodon spathula , A. baeri (Gallis et al 1991) and Scaphirhynchus platorynchus (Linhart et al 1995). It has been reported that a change in the external pH value induces a change in the internal pH.…”

“…Ca 2þ and Na þ of more than 10 mM had a negative effect on sperm motility. Sturgeon sperm is motile in the range 0 -120 mosmol kg 21 (Gallis et al 1991, Linhart et al 1995, but the osmotic level of seminal plasma is lower than 100 mosmol kg 21 (Gallis et al 1991, Piros et al 2002. These findings suggest that (1) control of sturgeon sperm motility is due to the ionic content of the medium but that (2) sturgeon sperm shows sensitivity to osmolality and ion concentrations of the medium.…”

“…Until now, little information has been available on the sperm biology of sturgeon, including ultrastructure and morphological functions (Cherr and Clark 1984, 1985, Ciereszko et al 1994, characterization of motility and fertilization ability (Gallis et al 1991, Linhart et al 1995, Tsvetkova et al 1996, Toth et al 1997, Williot et al 2000, respiration and energetics of motility of spermatozoa , Ingermann et al 2002, the correlation between spermatozoan motility parameters and biochemical characteristics of seminal plasma (Gallis et al 1991, Toth et al 1997, Ingermann et al 2002 and cryopreservation and short-term storage (Tsvetkova et al 1996, Jahnichen et al 1999. Unfortunately, data concerning biological aspects of sperm in the Persian sturgeon are rare .…”

“…In Salmonidae the period of active movement of spermatozoa decreases when the dilution rate decreases (Ginzburg 1968). Compared with teleosts, there are several studies show that Acipenseridae sperm becomes motile at low dilution rates (Gallis et al 1991, Linhart et al 1995, Toth et al 1997, Alavi et al 2002. Gallis et al (1991) reported that the duration of sperm motility and intensity of spermatozoa in A. baeri increased when the dilution rate increased from 1:6 to 1:100.…”

“…Motility of carp sperm is fully initiated in media of osmotic pressure below 150-200 mosmol kg 21 (Plouidy and Billard 1982), and the osmolality of seminal plasma is higher than necessary for activation of sperm; for example, the osmolality of carp seminal plasma is 286 mosmol kg 21 (Plouidy and Billard 1982). In the case of sturgeon, spermatozoa from Siberian sturgeon (Gallis et al 1991), shovelnose sturgeon (Linhart et al 1995) and paddlefish (Linhart et al 1995) were motile in a range of osmotic pressures; 0-100 mosmol kg…”