Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa

Billard, Roland; Cosson, Jacky; Fierville, F.; Brun, R.; Rouault, Thierry; Williot, Patrick

doi:10.1111/j.1439-0426.1999.tb00234.x

Cited by 55 publications

(40 citation statements)

References 12 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spermatozoa concentration was determined to be 0.28 ± 0.27 (·10 9 spz ml )1 ). As in the other chondrostean species (Billard et al, 1999;Cosson et al, 2000), spermatozoa motility of H. huso is longer than 5 min, and the velocities were stable during the early period (in 1 min) of sperm activation.…”

Section: Resultsmentioning

confidence: 84%

Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

Rodina

Hulák

et al. 2010

Journal of Applied Ichthyology

View full text Add to dashboard Cite

Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 ± 5.63 to 105.78 ± 5.27 lms )1 ) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 ± 0.16 mg ml )1 ), spermatozoa concentration (0.28 ± 0.27 · 10 9 spz ml )1 ), seminal plasma osmolality (51.33 ± 4.91 mOsmol kg )1 ), the pH (8.49 ± 0.01) and Na + (18.97 ± 3.65 mM M) , K + (2.83 ± 1.36 mM M), Ca 2+ (0.19 ± 0.06 mM M), Mg 2+ (0.49 ± 0.23 mM M) and Cl ) (6.33 ± 0.58 mM M) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and isoelectric points ranging from 5 to 9.5 were found. The observed physiological and biochemical properties, together with protein patterns, should be considered for the development of methods for controlled reproduction and sperm cryopreservation for the highly endangered beluga sturgeon.

show abstract

Section: Resultsmentioning

confidence: 84%

Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

Rodina

Hulák

et al. 2010

Journal of Applied Ichthyology

View full text Add to dashboard Cite

show abstract

“…2008). The inhibition of spermatozoa motility by moderate concentrations of K + can be overcome by an increase in external Ca 2+ concentration (Billard et al. 1999).…”

Section: Discussionmentioning

confidence: 99%

“…These characteristics play a crucial physio‐endocrinological role in supporting spermatozoa after release of sperm from the testis into the sperm duct and subsequently after discharge of sperm into the aquatic environment (Morisawa and Morisawa 1988). Spermatozoa concentration is low in sturgeon and paddlefish in comparison with teleosts, but the spermatozoa motility period is longer than that in teleosts, reported to be from 1 min to more than 5 min (Billard et al. 1999).…”

Section: Introductionmentioning

confidence: 99%

Spermatozoa Concentration, Seminal Plasma Composition and Their Physiological Relationship in the Endangered Stellate Sturgeon (Acipenser stellatus) and Russian Sturgeon (Acipenser gueldenstaedtii )

Rodina²,

Hulák³

et al. 2011

Reprod Domestic Animals

View full text Add to dashboard Cite

Comparative studies of ionic composition, osmolality, protein concentration and pH of seminal plasma along with spermatozoa concentrations were carried out in stellate sturgeon, Acipenser stellatus, and Russian sturgeon, Acipenser gueldenstaedtii. Analysis of A. gueldenstaedtii sperm showed significantly higher concentrations of Na(+) (34.58 ± 4.61 mm), Ca²(+) (0.35 ± 0.12 mm), Mg²(+) (0.70 ± 0.25 mm), Cl(-) (13.50 ± 4.04 mm) and proteins (0.60 ± 0.29 mg/ml) in the seminal plasma than did seminal plasma of A. stellatus: Na(+) (20.08 ± 10.75 mm), Ca²(+) (0.28 ± 0.06 mm), Mg²(+) (0.29 ± 0.05 mm), Cl(-) (7.50 ± 3.00 mm) and 0.30 ± 0.11 mg/ml proteins. Significantly higher concentration of K(+) (5.42 ± 1.06 mm) was observed in A. stellatus compared to A. gueldenstaedtii K(+) (2.29 ± 0.50 mm). Concentration of Na(+) was positively correlated with osmolality (r = 0.819), levels of Cl(-) (r = 0.922) and Mg²(+) (r = 0.727) and pH (r = 0.848). The concentration of Mg²(+) was positively correlated with protein concentration (r = 0.774), Na(+) (r = 0.727), Cl(-) (r = 0.872) and Ca²(+) (r = 0.801). A positive relationship was also found between concentration of K(+) and spermatozoa concentration (r = 0.709). Results revealed strong inter-species differences in several parameters. The data should be useful for artificial fertilization and for cryopreservation of sturgeon sperm.

show abstract

“…ATP content in fish spermatozoa is in the range between 6 and 24 nmol/(10 8 ) spermatozoa (Perchec et al 1995;Billard et al 1997Billard et al , 1999Dreanno et al 1999a,b). For carp spermatozoa, we published earlier (Rurangwa et al 2002) the ATP content about 10 nmol/(10 8 ) spermatozoa.…”

Section: Discussionmentioning

confidence: 97%

ATP Content and Adenine Nucleotide Catabolism in African Catfish Spermatozoa Stored in Various Energetic Substrates

Ziętara

Słomińska

Świerczyński

et al. 2004

Fish Physiol Biochem

View full text Add to dashboard Cite

Influence of various energetic substrates on ATP content and adenine nucleotide catabolism in African catfish, Clarias gariepinus spermatozoa was investigated. Present data indicate that sperm storage at 4°C in the presence of different energetic substrates leads to time dependent ATP depletion. Among the substrates studied (i.e. glycine, glucose, or lactate plus pyruvate), the lactate plus pyruvate seems to be the most favourable substrate to maintain ATP concentration. ATP depletion is associated with increase of the AMP content, while ADP concentration is changing only slightly. Moreover, significant increase in hypoxanthine content both in the sperm and a storage medium was found. Adenine nucleotide energy charge (AEC) decreased significantly during the first 24 h when sperm was stored in the presence of glycine. During the same period, only slight decrease in AEC was observed in spermatozoa stored in the presence of glucose and no significant changes were found in the presence of lactate plus pyruvate. A further decrease in AEC was observed until 120 h of storage in the medium containing glycine, lactate plus pyruvate or glucose. However, the changes were relatively small when spermatozoa were stored in the presence of lactate plus pyruvate. Important finding is that in contrast to the results reported recently, AEC value in fresh milt (at 0 time of storage) was in the normal range. The present study shows that African catfish spermatozoa are able to maintain physiological level of ATP and AEC when they are stored in the presence of favourable energetic substrates. In conclusion, the results of this investigation suggest that successful short-term storage of African catfish milt depends on energetic substrates supply.Abbreviations: AEC -adenine nucleotide energy charge; ADP -adenosine diphosphate; AMP -adenosine monophosphate; ATP -adenosine triphosphate; cAMP -cyclic AMP; HPLC -high performance liquid chromatography; IMP -inosine monophosphate; TAN -total adenine nucleotide.

show abstract

Motility analysis and energetics of the Siberian sturgeon Acipenser baerii spermatozoa

Cited by 55 publications

References 12 publications

Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

Spermatozoa Concentration, Seminal Plasma Composition and Their Physiological Relationship in the Endangered Stellate Sturgeon (Acipenser stellatus) and Russian Sturgeon (Acipenser gueldenstaedtii )

ATP Content and Adenine Nucleotide Catabolism in African Catfish Spermatozoa Stored in Various Energetic Substrates

Contact Info

Product

Resources

About