Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

Abstract

Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 ± 5.63 to 105.78 ± 5.27 lms )1 ) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 ± 0.16 mg ml )1 ), spermatozoa concentration (0.28 ± 0.27 · 10 9 spz ml )1 ), seminal plasma osmolality (51.33 ± 4.91 mOsmol kg )1 ), the pH (8.49 ± 0.01) and Na + (18.97 ± 3.65 mM M) , K + (2.83 ± 1.36 mM M), Ca 2+ (0.19 ± 0.06 mM M), Mg 2+ (0.49 ± 0.23 mM M) and Cl ) (6.33 ± 0.58 mM M) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and isoelectric points ranging from 5 to 9.5 were found. The observed physiological and biochemical properties, together with protein patterns, should be considered for the development of methods for controlled reproduction and sperm cryopreservation for the highly endangered beluga sturgeon.

Evaluation of Spermiation Indices with Multiple Sperm Collections in Endangered Sterlet (Acipenser ruthenus)

“…1990), but in agreement with concentrations reported for sturgeon (Piros et al. 2002; Li et al. 2009, 2010).…”

“…The impact of seminal plasma proteins on sperm function is complex and not fully understood. Accumulating evidence shows that seminal plasma plays a pivotal role in the regulation of sperm motility and in the protection against proteolytic attack during storage of spermatozoa in the reproductive system (Li et al. 2010).…”

Comparison of Protein Fractions in Seminal Plasma from Multiple Sperm Collections in Sterlet (Acipenser ruthenus)

“…Sperm samples were initially centrifuged at 300 · g for 30 min followed by 10 min at 16 000 · g at 4°C according to the methods described by Li et al (2010b). The supernatant was collected and stored at )80°C until analysis.…”

Effect of season on proteases and serine protease inhibitors of Siberian sturgeon (Acipenser baeriiBrandt, 1869) semen

“…The latter possess spermatozoa that lack an acrosome and are simplified structurally. Compared to that of teleost fish, the sperm concentration of semen is low, usually about 1 x 10 9 spermatozoa ml À1 (Piros et al, 2002;Li et al, 2010Li et al, , 2011aShaliutina et al, 2012). The sturgeon acrosome seems to be functional, because the acrosome reaction (AR) has been described for this species despite the presence of micropyles in the eggs (Ginsburg, 1968;Cherr and Clark, 1984;Alavi et al, 2012b).…”

“…Contrary to those of teleost fish, sturgeon spermatozoa are bigger, relatively complex and have an acrosome (Dettlaff et al, 1993). Among the most characteristic features sturgeon seminal plasma are of low osmolality (about 100 mOsm kg À1 or less) and protein concentration, about 1 mg ml À1 or less (Piros et al, 2002;Li et al, 2010Li et al, , 2011aAlavi et al, 2012a;Shaliutina et al, 2012). Compared to that of teleost fish, the sperm concentration of semen is low, usually about 1 x 10 9 spermatozoa ml À1 (Piros et al, 2002;Li et al, 2010Li et al, , 2011aShaliutina et al, 2012).…”