Most LAM Mycobacterium tuberculosis strains in Venezuela, but not SIT605, belong to the RDRio subfamily

Méndez, María Victoria; Abadia, Edgar; Sequera, Mónica; Waard, Jacobus H. de; Takiff, Howard

doi:10.1016/j.meegid.2020.104380

Cited by 3 publications

(5 citation statements)

References 29 publications

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“…Second, our study was done with samples from years 2012–2016, prior to the massive Venezuelan migration that arrived to Ecuador since 2018 following this country economic collapse ( 52 , 53 ). Considering that the highest prevalence of MTB RD Rio in South America has been described for Venezuela ( 19 ), the current scenario for MTB RD Rio prevalence in Ecuador could have changed due to importation of cases and further studies with MTB strains collection from recent years are needed.…”

Section: Discussionmentioning

confidence: 99%

“…This LAM variant, called MTB RD Rio is associated with multidrug resistance and distributed worldwide ( 22 , 24 – 27 ). The prevalence of MTB RD Rio varies in Latin America: 30 to 51.9% in Brazil ( 22 , 28 ), 69.8% in Venezuela ( 19 ), 37% in Colombia ( 17 , 21 , 29 ), 31% in Peru ( 21 , 30 ), 20% in Argentina ( 21 ), 10% in Paraguay ( 18 ) and 3% in Chile ( 21 ). However, there are no reports of the RD Rio in Ecuador.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence, drug resistance, and genotypic diversity of the RDRio subfamily of Mycobacterium tuberculosis in Ecuador: a retrospective analysis for years 2012–2016

Castro-Rodriguez,

Franco-Sotomayor,

Benitez-Medina

et al. 2024

Front. Public Health

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IntroductionA major sublineage within the Mycobacterium tuberculosis (MTB) LAM family characterized by a new in-frame fusion gene Rv3346c/55c was discovered in Rio de Janeiro (Brazil) in 2007, called RDRio, associated to drug resistance. The few studies about prevalence of MTB RDRio strains in Latin America reported values ranging from 3% in Chile to 69.8% in Venezuela, although no information is available for countries like Ecuador.MethodsA total of 814 MTB isolates from years 2012 to 2016 were screened by multiplex PCR for RDRio identification, followed by 24-loci MIRU-VNTR and spoligotyping.ResultsA total number of 17 MTB RDRio strains were identified, representing an overall prevalence of 2.09% among MTB strains in Ecuador. While 10.9% of the MTB isolates included in the study were multidrug resistance (MDR), 29.4% (5/17) of the RDRio strains were MDR.DiscussionThis is the first report of the prevalence of MTB RDRio in Ecuador, where a strong association with MDR was found, but also a very low prevalence compared to other countries in Latin America. It is important to improve molecular epidemiology tools as a part of MTB surveillance programs in Latin America to track the transmission of potentially dangerous MTB stains associated to MDR TB like MTB RDRio.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence, drug resistance, and genotypic diversity of the RDRio subfamily of Mycobacterium tuberculosis in Ecuador: a retrospective analysis for years 2012–2016

Castro-Rodriguez,

Franco-Sotomayor,

Benitez-Medina

et al. 2024

Front. Public Health

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“…The RDRio genotype is remarkably frequent in Brazil, although its prevalence varies widely between regions (15.5% to 52%) [38,39]. In Venezuela it was reported in almost 70% of LAM strains [40]. However, it appears to have a limited contribution to the TB burden in Argentina and Paraguay, even though they share a border with Brazil.…”

Section: Genotypes Associated With Euro-american Lineage In Latin Ame...mentioning

confidence: 99%

“…Analyses of 45 SNPs showed that it belongs to the LAM family, and due to its geographical distribution, it was named "Carabobo" [42]. Méndez et al [40] inferred, by using spoligotyping and 24-loci MIRU-VNTR, that the SIT605 genotype could belong to the LAM1 subfamily, and evidenced that none of the SIT605 strains harbored the RDRio genotype.…”

Section: Genotypes Associated With Euro-american Lineage In Latin Ame...mentioning

confidence: 99%

“…The 24-loci MIRU-VNTR method confirmed the designation of the Carabobo genotype as belonging to the LAM family. Additionally this method demonstrated that the strains harboring the SIT1698, specific to Venezuela, seem to belong to the Carabobo genotype, since they shared the same genotype profile [40,42,44].…”

Section: Genotypes Associated With Euro-american Lineage In Latin Ame...mentioning

confidence: 99%

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Genotyping methods and their contributions to the study of tuberculosis dynamic in Latin America

Castillos de Ibrahim das Neves,

Reis,

Xavier Maio

et al. 2023

J Infect Dev Ctries

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Introduction: Mycobacterium tuberculosis genotyping has impacted evolutionary studies worldwide. Nonetheless, its application and the knowledge generated depend on the genetic marker evaluated and the detection technologies that have evolved over the years. Here we describe the timeline of main genotypic methods related to M. tuberculosis in Latin America and the main findings obtained. Methodology: Systematic searches through the PubMed database were performed from 1993 to May 2021. A total of 345 articles met the inclusion criteria and were selected. Results: Spacer oligonucleotide typing (spoligotyping) was the most widely used method in Latin America, with decreasing use in parallel with increasing use of mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) and whole genome sequencing (WGS). Among the countries, Brazil, Mexico, and Argentina had the most publications, and a considerable part of the articles were in collaboration with Latin American or non-Latin American institutions; a small proportion of studies needed partnerships to perform the genotypic methods. The genotypic methods allowed the identification of M. tuberculosis genotypes with greater capacity for clonal expansion and revealed the predominance of the Euro-American lineage in Latin America. There was a notable presence of the Beijing family in Peru and Colombia. Conclusions: The data obtained demonstrated the importance of expanding collaborative networks of tuberculosis (TB) research groups to countries with low productivity in this area, the commitment of the few Latin American countries to advance TB research, as well as the inestimable value of building a Latin America database, considering ease of population mobility between countries.

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Molecular epidemiology of Mycobacterium tuberculosis in Ecuador: Recent advances and future challenges

Castro-Rodriguez,

Franco-Sotomayor,

Orlando

et al. 2024

Journal of Clinical Tuberculosis and Other Mycobacterial Diseas

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Most LAM Mycobacterium tuberculosis strains in Venezuela, but not SIT605, belong to the RDRio subfamily

Cited by 3 publications

References 29 publications

Prevalence, drug resistance, and genotypic diversity of the RDRio subfamily of Mycobacterium tuberculosis in Ecuador: a retrospective analysis for years 2012–2016

Prevalence, drug resistance, and genotypic diversity of the RDRio subfamily of Mycobacterium tuberculosis in Ecuador: a retrospective analysis for years 2012–2016

Genotyping methods and their contributions to the study of tuberculosis dynamic in Latin America

Molecular epidemiology of Mycobacterium tuberculosis in Ecuador: Recent advances and future challenges

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