Mössbauer spectroscopic study of transformations of iron species by the cyanobacterium Arthrospira platensis (formerly Spirulina platensis)

Perfiliev, Yu. D.; Тамбиев, А.Х.; Konnychev, Maxim A.; Skalny, Anatoly V.; Лобакова, Е. С.; Kirpichnikov, Mikhaǐl P.

doi:10.1016/j.jtemb.2018.02.030

Cited by 10 publications

(9 citation statements)

References 14 publications

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“…The percentage of the iron content determined in A. platensis was in the range of data reported by Isani et al (2022 b) [ 28 ] in the same species. The data in the present study provided additional evidence as to what had been hypothesised by Perfiliev et al (2018) [ 35 ] that A. platensis accumulated iron, for the most part in an inorganic form such as ferrihydrite. An even higher iron content in pellets was found in C. vulgaris , H. pluvialis , and P. tricornutum , with percentages from 92 to 97%.…”

Section: Resultssupporting

confidence: 89%

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Dalmonte

Vecchiato

Biagi

et al. 2023

Veterinary Sciences

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Chlorella vulgaris, Arthrospira platensis, Haematoccocus pluvialis, and Phaeodactylum tricornutum are species of interest for commercial purposes due to their valuable nutritional profile. The aim of this study was to investigate the iron content in these four microalgae, with emphasis on their iron bioaccessibility assessed using an in vitro digestion system to simulate the process which takes place in the stomach and small intestine of dogs, followed by iron quantification using atomic absorption spectrometry. Furthermore, the extraction of soluble proteins was carried out and size exclusion chromatography was applied to investigate iron speciation. Significant differences (p < 0.004) in iron content were found between C. vulgaris, which had the highest (1347 ± 93 μg g−1), and H. pluvialis, which had the lowest (216 ± 59 μg g−1) iron content. C. vulgaris, A. platensis, and H. pluvialis showed an iron bioaccessibility of 30, 31, and 30%, respectively, while P. tricornutum showed the lowest bioaccessibility (11%). The four species analysed presented soluble iron mainly bound to proteins with high molecular mass ranging from >75 to 40 kDa. C. vulgaris showed the highest iron content associated with good bioaccessibility; therefore, it could be considered to be an interesting natural source of organic iron in dog nutrition.

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Section: Resultssupporting

confidence: 89%

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Dalmonte

Vecchiato

Biagi

et al. 2023

Veterinary Sciences

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“…On average, daily supplementation with the most commonly recommended dose of 3.0 g would constitute 25% of AI with some Spirulina and Chlorella products reaching over 60 and 40% of AI, respectively. As found in previous studies, bioavailability of Fe from Spirulina biomass (in which it is mostly present as ferrihydrite) is lower than that of ferrous sulfate, often used in mineral food supplements, but higher than that in whole wheat (Kapoor and Mehta 1992;Perfiliev et al 2018). However, in the case of Fe-fortified Spirulina, mineral bioavailability can be even higher than that from meat products (Puyfoulhoux et al 2001).…”

Section: Discussionmentioning

confidence: 63%

Essential and toxic elements in commercial microalgal food supplements

et al. 2018

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Arthrospira spp. (known commercially as Spirulina) and Chlorella spp., valued for their evidence-based nutritional and bioactive properties, are cultivated for the purpose of production of food supplements for worldwide distribution. However, the quality and safety of the final product depends on culturing and manufacturing conditions. The present study investigated the content of toxic elements (As species, Al, Cd, Hg, Ni, Pt, Pb, Cr (VI), rare earth elements) and minerals (Ca, Co, Cr, Cu, Fe, K, Na, Mg, Mn, P, Zn) in Chlorella (n = 10) and Spirulina (n = 13) food supplements registered in the European Union. Considering the most common recommended daily dosage 3.0, supplementation with any of the studied product would contribute significantly to mineral intake, with the exception of Fe which was found at high but acceptable levels in both Spirulina and Chlorella formulas. The majority of products revealed agreement between factual mineral content and that declared on the label, the only exception being Cu content in Chlorella products found to be significantly higher (> 130%). All studied supplements were found to have Cd, Hg, and Ni levels much below safety limits, although selected ones were characterized by increased content of Al, Pb, and inorganic As. No hexavalent Cr was detected in the studied products. The study highlights that microalgal supplements can be safe for consumers if appropriate measures are taken to ensure consumer safety, although it underlines the continuous need to monitor these products in order to fully eliminate those of low quality.

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“…It has been hypothesized that iron precipitates as FePO 4, determining the decrease in the metal concentration available for cell growth [ 14 ]. Moreover, it has been reported by Perfiliev et al (2018) that iron concentrations in the culture medium higher than 5 µg L −1 causes sedimentation of the metal, determining a lower bioaccessibility of iron [ 15 ]. In the present study, the discrepancy between the iron nominal concentration and the concentration determined in culture media by AAS suggests iron precipitation.…”

Section: Discussionmentioning

confidence: 99%

“…On average, for biomass obtained at the highest iron concentration in this research, daily supplementation with the commonly recommended dietary intake of 3.0 g of spirulina would constitute 64% of the population reference intake (PRI) of 11 mg day −1 reported by EFSA for iron [ 19 ]. It has been reported that A. platensis trichomes accumulate iron mostly in the form of Fe 3+ as ferrihydrite [ 15 ]. Therefore, the bulk of iron present in the pellet of all the examined samples should be stored in this inorganic form.…”

Section: Discussionmentioning

confidence: 99%

Iron Speciation and Iron Binding Proteins in Arthrospira platensis Grown in Media Containing Different Iron Concentrations

Isani

Niccolai

Andreani

et al. 2022

IJMS

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Cyanobacteria are characterized by high iron content. This study investigated the effects of varying iron concentrations (1, 5, and 10 mg L−1) in the culture media on the biochemical composition and the iron bioaccumulation and speciation in Arthrospira platensis F&M-C256. Iron content measured in biomasses varied from 0.35 to 2.34 mg g−1 dry weight depending on the iron concentration in the culture media. These biomasses can be considered of interest for the production of spirulina-based supplements with low and high iron content. Iron speciation was studied using size exclusion chromatography followed by atomic absorption spectrometry and proteomic analysis. The role of C-phycocyanin as an iron binding protein was also investigated. Overall, the present results provide a better understanding of iron metabolism in cyanobacteria and a foundation for further studies.

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Mössbauer spectroscopic study of transformations of iron species by the cyanobacterium Arthrospira platensis (formerly Spirulina platensis)

Cited by 10 publications

References 14 publications

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Iron Bioaccessibility and Speciation in Microalgae Used as a Dog Nutrition Supplement

Essential and toxic elements in commercial microalgal food supplements

Iron Speciation and Iron Binding Proteins in Arthrospira platensis Grown in Media Containing Different Iron Concentrations

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