Mosquitoes infected with dengue viruses in Brazil

Abstract

Dengue epidemics have been reported in Brazil since 1985. The scenery has worsened in the last decade because several serotypes are circulating and producing a hyper-endemic situation, with an increase of DHF/DSS cases as well as the number of fatalities. Herein, we report dengue virus surveillance in mosquitoes using a Flavivirus genus-specific RT-Hemi-Nested-PCR assay. The mosquitoes (Culicidae, n = 1700) collected in the Northeast, Southeast and South of Brazil, between 1999 and 2005, were grouped into 154 pools. Putative genomes of DENV-1, -2 and -3 were detected in 6 mosquito pools (3.8%). One amplicon of putative DENV-1 was detected in a pool of Haemagogus leucocelaenus suggesting that this virus could be involved in a sylvatic cycle. DENV-3 was found infecting 3 pools of larvae of Aedes albopictus and the nucleotide sequence of one of these viruses was identified as DENV-3 of genotype III, phylogenetically related to other DENV-3 isolated in Brazil. This is the first report of a nucleotide sequence of DENV-3 from larvae of Aedes albopictus.

Genetic characterization of dengue viruses prevalent in Kerala State, India

“…Kerala has an abundant Ae. albopictus population (Kumar et al, 2011), an established vector for dengue fever in Asia (De Figueiredo et al, 2010;Vasilakis et al, 2011), and restricted prevalence of Ae. aegypti in urban situations.…”

Identification of dengue viruses in naturally infected Aedes aegypti females captured with BioGents (BG)-Sentinel traps in Manaus, Amazonas, Brazil

“…The presence of DENV has been previously detected in A. aegypti and other mosquito species 8 , and DENV co-infections have been reported in clinical samples 9 . These findings of the present study in A. aegypti mosquitoes corroborate the detection of DENV in clinical samples and reinforces the need for strengthened epidemiological surveillance of dengue infection by the Health authorities.…”

Estudo da resposta de mosquitos (Diptera:Culicidae) a diferentes tipos de criadouros e condições de fatores físico-químicos da água

“…A espécie Aedes albopictus já foi encontrada naturalmente infectada com vírus da dengue (Ibáñez-Bernal et al, 1997;Figueiredo et al, 2010), febre amarela (Instituto Evandro Chagas, 2018) e é considerada vetora do vírus Chikungunya (Organização Mundial da Saúde, 2017). Esta espécie coloniza os mais diversos tipos de criadouros artificiais e naturais, apresentando-se como generalista no que diz respeito à seleção de recipientes para oviposição, em ambientes que podem ir de preservados a totalmente modificados (Consoli & Lourenço-de-Oliveira, 1994).…”