Mosquitocidal potential of silver nanoparticles synthesized using local isolates of Bacillus thuringiensis subsp. israelensis and their synergistic effect with a commercial strain of B. thuringiensis subsp. israelensis

Thammasittirong, Anon; Prigyai, Kanchana; Thammasittirong, Sutticha Na-Ranong

doi:10.1016/j.actatropica.2017.07.020

Cited by 5 publications

(10 citation statements)

References 35 publications

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“…The inclusion proteins of the mixed toxins and secreted metabolites of Bti were prepared according to our previous report [ 17 ]. The expression of the mixed toxins in Bti was analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).…”

Section: Methodsmentioning

confidence: 99%

“…The biological-based green synthesis NPs have many advantages, including being simple, rapid, non-toxic, reproducible, easy to scale up, and cost-effective, compared to chemical and physical syntheses. In addition, the biological-based NPs have been reported to be more effective, probably as a result of the attachment of biologically active components on the surface of the synthesized NPs [ 15 , 16 , 17 ]. The chemical composition of NPs, such as Ag + that is released from silver nanoparticles (AgNPs), preferentially bind to a sulfhydryl (-SH) group containing proteins, leading to cytotoxicity [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…NPs can promote the production of intracellular reactive oxygen species (ROS) that cause serious cellular damage and apoptosis [ 18 , 19 ]. In addition, various bioactivities of NPs have been reported, including antimicrobial, antioxidant, anti-inflammatory, anticancer, and insecticidal activities [ 9 , 10 , 14 , 17 ]. Silver/silver chloride nanoparticles (Ag/AgCl NPs) synthesized from various extracts of plants and microorganisms have been reported to contain high larvicidal activity against A. aegypti larvae [ 17 , 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, various bioactivities of NPs have been reported, including antimicrobial, antioxidant, anti-inflammatory, anticancer, and insecticidal activities [ 9 , 10 , 14 , 17 ]. Silver/silver chloride nanoparticles (Ag/AgCl NPs) synthesized from various extracts of plants and microorganisms have been reported to contain high larvicidal activity against A. aegypti larvae [ 17 , 20 , 21 ]. Damage to the A. aegypti larval midgut, epithelial cells, and cortex results in physiological changes, such as shrinkage in the abdominal region, thorax shape change, and loss of lateral hairs, anal gills, and brushes, which have been reportedly caused by green synthesis NPs [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

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Proteomic Response of Aedes aegypti Larvae to Silver/Silver Chloride Nanoparticles Synthesized Using Bacillus thuringiensis subsp. israelensis Metabolites

Chimkhan

Thammasittirong

Roytrakul

et al. 2022

Insects

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Silver/silver chloride nanoparticles (Ag/AgCl NPs) are an alternative approach to control the larvae of Aedes aegypti, a vector of mosquito-borne diseases. However, the molecular mechanisms of Ag/AgCl NPs to A. aegypti have not been reported. In this work, Ag/AgCl NPs were synthesized using supernatant, mixed toxins from Bacillus thuringiensis subsp. israelensis (Bti), and heterologously expressed Cry4Aa and Cry4Ba toxins. The images from scanning electron microscopy revealed that the Ag/AgCl NPs were spherical in shape with a size range of 25–100 nm. The larvicidal activity against A. aegypti larvae revealed that the Ag/AgCl NPs synthesized using the supernatant of Bti exhibited higher toxicity (LC50 = 0.133 μg/mL) than the Ag/AgCl NPs synthesized using insecticidal proteins (LC50 = 0.148–0.217 μg/mL). The proteomic response to Ag/AgCl NPs synthesized using the supernatant of Bti in A. aegypti larvae was compared to the ddH2O-treated control. Two-dimensional gel electrophoresis analysis revealed 110 differentially expressed proteins, of which 15 were selected for identification using mass spectrometry. Six upregulated proteins (myosin I heavy chain, heat shock protein 70, the F0F1-type ATP synthase beta subunit, methyltransferase, protein kinase, and condensin complex subunit 3) that responded to Ag/AgCl NP treatment in A. aegypti were reported for NP treatments in different organisms. These results suggested that possible mechanisms of action of Ag/AgCl NPs on A. aegypti larvae are: mitochondrial dysfunction, DNA and protein damage, inhibition of cell proliferation, and cell apoptosis. The findings from this work provide greater insight into the action of green synthesized Ag/AgCl NPs on the control of A. aegypti larvae.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Proteomic Response of Aedes aegypti Larvae to Silver/Silver Chloride Nanoparticles Synthesized Using Bacillus thuringiensis subsp. israelensis Metabolites

Chimkhan

Thammasittirong

Roytrakul

et al. 2022

Insects

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“…In addition to lepidopteran insects, the selected B. thuringiensis isolates were assayed for toxicity against the larvae of a dipteran insect, Aedes aegypti, according to Thammasittirong et al (2017). A. aegypti larvae were hatched from eggs supplied by the Ministry of Public Health, Thailand.…”

Section: Insecticidal Activity Assaysmentioning

confidence: 99%

Molecular characterization of lepidopteran-specific toxin genes in Bacillus thuringiensis strains from Thailand

Boonmee

Thammasittirong

2019

3 Biotech

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A total of 511 local isolates of Bacillus thuringiensis from different geographical regions of Thailand were analyzed for the presence of the cry1A, cry1B, cry2A, cry9, and vip3A genes encoding for lepidopteran-specific toxins. PCR results revealed that 94.32% (482/511) of B. thuringiensis isolates harbored at least one of the detected genes, of which the cry1A, cry1B, cry2A, cry9, and vip3A genes were detected at frequencies of 90.61%, 89.63%, 76.32%, 40.70%, and 48.18%, respectively. Nineteen gene-combination profiles were discovered among 482 B. thuringiensis isolates, of which the most frequently detected profile contained the cry1A, cry1B, cry2A, and vip3A genes. Sixty-one isolates (12.66%), which harbored all of the detected insecticidal toxin genes, were further detected for the exochitinase (chi36) gene and chitinase activity. The results revealed that all 61 isolates contained the chi36 gene and exhibited chitinase activity. Insect bioassays showed that five isolates were highly toxic (more than 80% mortality) against second instar larvae of Spodoptera litura, of which the highest insect mortality (93%) was obtained from the B. thuringiensis isolates 225-15 and 417-1. Scanning electron microscopy revealed that the crystal morphologies of the five effective isolates were bipyramidal and cuboidal shapes. SDS-PAGE analysis of the spore-crystal mixture showed major bands of approximately 65 and 130 kDa. These five effective strains are alternative candidates for use as a microbial insecticide for the control of the S. litura pest.

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Anti-plasmodial and mosquitocidal potential of metallic nanoparticles: a perspective

Kulkarni

Mohanty

Bhagit

et al. 2022

Proc.Indian Natl. Sci. Acad.

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Mosquitocidal potential of silver nanoparticles synthesized using local isolates of Bacillus thuringiensis subsp. israelensis and their synergistic effect with a commercial strain of B. thuringiensis subsp. israelensis

Cited by 5 publications

References 35 publications

Proteomic Response of Aedes aegypti Larvae to Silver/Silver Chloride Nanoparticles Synthesized Using Bacillus thuringiensis subsp. israelensis Metabolites

Proteomic Response of Aedes aegypti Larvae to Silver/Silver Chloride Nanoparticles Synthesized Using Bacillus thuringiensis subsp. israelensis Metabolites

Molecular characterization of lepidopteran-specific toxin genes in Bacillus thuringiensis strains from Thailand

Anti-plasmodial and mosquitocidal potential of metallic nanoparticles: a perspective

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