Proteomic Response of Aedes aegypti Larvae to Silver/Silver Chloride Nanoparticles Synthesized Using Bacillus thuringiensis subsp. israelensis Metabolites

Chimkhan, Nantipat; Thammasittirong, Sutticha Na-Ranong; Roytrakul, Sittiruk; Krobthong, Sucheewin; Thammasittirong, Anon

doi:10.3390/insects13070641

Cited by 5 publications

(5 citation statements)

References 70 publications

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“…The possible mechanism of action of Ag/AgCl NPs against larvae of A. aegypti is through mitochondrial dysfunction, DNA and protein damage, inhibited cell proliferation, and cell apoptosis. Thus, Ag/AgCl NPs are recommended as an alternative method to control A. aegypti, a mosquito-borne disease vector (Chimkhan et al 2022). So that B. subtilis strain LS9.1 results from this study can also be used for the synthesis of green Ag-NPs and prospectively as a biocontrol agent to reduce the A. aegypti mosquito population.…”

Section: Discussionmentioning

confidence: 86%

“…Geetha et al (2012) reported the need to improve the mosquito adulticidal activity of biosurfactants produced by B. subtilis subtilis. B. subtilis also has been developed as a microbial agent for the green synthesis of silver nanoparticles, a perspective for the control of A. aegypti larvae (Wilson et al 2022;Chimkhan et al 2022). The other studies reported by Bais et al (2004), Fira et al (2018 and Kumbar et al (2019) showed that B. subtilis could be developed for plant pathogens biocontrol agent.…”

Section: Introductionmentioning

confidence: 99%

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Prospects of Indigenous Bacillus subtilis Strain LS9.1 as a Potential Biocontrol Agent against Aedes aegypti Larvae

Salamun,

Ni'matuzahroh,

Fatimah

et al. 2023

HAYATI J Biosci

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Biocontrol is one way that can be developed to overcome public health and agricultural problems. Biocontrol agents could be a microorganism, for example, from the Bacillus group. This study aims to determine the larvicidal toxicity of the Bacillus subtilis strain LS9.1 against Aedes aegypti larvae. The mode of action of the isolate caused larval death, which was detected with parasporal inclusions and secondary metabolites. Larvicidal toxicity with the bioassay method. Parasporal inclusion proteins were detected with the SDS-PAGE method. The Cry toxin coding gene with the PCR method and cell ultrastructure with transmission and scanning electron microscopy (TEM and SEM). The action of secondary metabolites was detected with hemolytic activity test. The isolate high toxicity to A. aegypti larvae. Parasporal inclusion proteins with molecular weights ranging from 72-7 kDa and the gene encoding a protein toxin in size 300 bp. The TEM and SEM results did not Cry-IV toxin. The hemolytic activity test showed a positive reaction correlated with biosurfactant production. Based on this study, the death of larvae is probably caused by secondary metabolites produced by Bacillus subtilis strain LS9.1. Thus, this isolate has the prospect of being an environmentally friendly biocontrol agent to disease vectors and insect pests.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Prospects of Indigenous Bacillus subtilis Strain LS9.1 as a Potential Biocontrol Agent against Aedes aegypti Larvae

Salamun,

Ni'matuzahroh,

Fatimah

et al. 2023

HAYATI J Biosci

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“…Iftikhar et al (2018), recommended the development of silver nanoparticles produced by B. mojavensis BTCB15, which may play a role in combating mosquito populations, thereby controlling vector-borne diseases. The larvicidal activity of silver nanoparticles (Ag/AgCl NPs) synthesized using Bti supernatant showed that LC50=0.133 g/mL, higher toxicity than that synthesized using insecticidal protein, LC50=0.148-0.217 g/mL (Chimkhan et al 2022). Possible mechanism of action of Ag/AgCl NPs against A. aegypti larvae is through mitochondrial dysfunction, DNA and protein damage, inhibition of cell proliferation, and cell apoptosis, and recommended that Ag/AgCl NPs are an alternative approach to control A. aegypti larvae, a mosquito-borne disease vector (Chimkhan et al 2022).…”

Section: Discussionmentioning

confidence: 99%

Potential biocontrol agent of indigenous Bacillus sp. EG6.4: Molecular identification, larvicidal toxicity, and mechanism of actions

et al. 2022

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Abstract. Salamun, Susetyo RD, Nafidiastri FA, Zain RA, Sari RP, Geraldi A, Fatimah, Ni’matuzahroh. 2022. Potential biocontrol agent of indigenous Bacillus sp. EG6.4: Molecular identification, larvicidal toxicity, and mechanism of actions Biodiversitas 23: 5431-5438. This research was carried out for molecular identification, as well as the determination and mechanism of action of larvicidal toxicity of Bacillus sp. EG6.4 isolated from breeding sites of Aedes aegypti from Gresik, East Java, Indonesia. Bacillus sp. EG6.4 was a Gram-positive endospore-forming bacteria. Molecular species identification using 16S rRNA gene sequencing showed that isolate had 97.89% similarity with Bacillus mojavensis. The isolate showed larvicidal toxicity against A. aegypti larvae. The lethal concentration 50% (LC50) values ??at 24 and 48hours exposure were 8.99±1.01 ×107 cells/mL and 8.43±1.01 ×107 cells/mL, respectively, while lethal time 50% (LT50) value was 11.9±1.1 hours. Production of chitinolytic enzymes or biosurfactants, chitinolytic and hemolytic assays were conducted to determine the larvicidal mechanism. As a result, Bacillus sp. EG6.4 showed hemolytic, but not chitinolytic activity, indicating its potency to produce biosurfactants. Transmission Electron Microscopy (TEM) result showed that isolate had oval-shaped endospores located subterminal with massive-shape parasporal inclusions. The detection of srfA-D gene showed that isolate produced surfactin biosynthesis thioesterase. Thus, Bacillus sp. EG6.4 produced biosurfactant that potentially to be developed as a biocontrol agent for disease vectors and plant pathogens.

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“…When silenced, larvae were much more tolerant of the toxin, indicating an interesting assistive effect of this protein with the toxin. However, another study found that 2 HSP70 proteins were upregulated in larvae treated with nanoparticles to deliver the Cry4Aa toxin ( 44 ). This may reflect differences in response to the specific toxins.…”

Section: Heat Shock Protein Gene Induction By Insecticidementioning

confidence: 99%

Heat shock proteins, thermotolerance, and insecticide resistance in mosquitoes

Mack,

Attardo

2024

Front. Insect Sci.

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Mosquitoes transmit pathogens that pose a threat to millions of people globally. Unfortunately, widespread insecticide resistance makes it difficult to control these public health pests. General mechanisms of resistance, such as target site mutations or increased metabolic activity, are well established. However, many questions regarding the dynamics of these adaptations in the context of developmental and environmental conditions require additional exploration. One aspect of resistance that deserves further study is the role of heat shock proteins (HSPs) in insecticide tolerance. Studies show that mosquitoes experiencing heat stress before insecticide exposure demonstrate decreased mortality. This is similar to the observed reciprocal reduction in mortality in mosquitoes exposed to insecticide prior to heat stress. The environmental shifts associated with climate change will result in mosquitoes occupying environments with higher ambient temperatures, which could enhance existing insecticide resistance phenotypes. This physiological relationship adds a new dimension to the problem of insecticide resistance and further complicates the challenges that vector control and public health personnel face. This article reviews studies illustrating the relationship between insecticide resistance and HSPs or hsp genes as well as the intersection of thermotolerance and insecticide resistance. Further study of HSPs and insecticide resistance could lead to a deeper understanding of how environmental factors modulate the physiology of these important disease vectors to prepare for changing climatic conditions and the development of novel strategies to prevent vector-borne disease transmission.

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Proteomic Response of Aedes aegypti Larvae to Silver/Silver Chloride Nanoparticles Synthesized Using Bacillus thuringiensis subsp. israelensis Metabolites

Cited by 5 publications

References 70 publications

Prospects of Indigenous Bacillus subtilis Strain LS9.1 as a Potential Biocontrol Agent against Aedes aegypti Larvae

Prospects of Indigenous Bacillus subtilis Strain LS9.1 as a Potential Biocontrol Agent against Aedes aegypti Larvae

Potential biocontrol agent of indigenous Bacillus sp. EG6.4: Molecular identification, larvicidal toxicity, and mechanism of actions

Heat shock proteins, thermotolerance, and insecticide resistance in mosquitoes

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