Mosquitocidal activity of penfluridol as juvenile hormone antagonist

Lee, Seok-Hee; Lim, Hee Nam; Choi, Jae Young; Park, Dong Hwan; Ahn, Byung Hoon; Fāng, Yīng; Kim, Jong Hoon; Park, Min Gu; Woo, Ra Mi; Lee, Bo Ram; Kim, Woo Jin; Ko, Yongmin; Lee, Ill Young; Je, Yeon Ho

doi:10.1016/j.aspen.2017.11.009

Cited by 7 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, many studies have proven that a variety of chemicals has been screened as JH agonists (Abe, Watanabe, et al, 2015; Tatarazako et al, 2003). On the other hand, screening of JH antagonists has not been advanced, although a few chemicals have been found using the yeast two‐hybrid system of mosquito JH receptor as a reporter system (Lee et al, 2015, 2018). Recently, a high‐sensitivity reporter system in mammalian cells for detecting juvenoids using JH receptors of Daphnia has been established (Miyakawa & Iguchi, 2017; Tanaka, Iguchi, & Miyakawa, 2019).…”

Section: Discussionmentioning

confidence: 99%

Methyl farnesoate regulatory mechanisms underlying photoperiod‐dependent sex determination in the freshwater crustacean Daphnia magna

Toyota

Satō

Iguchi

et al. 2020

J of Applied Toxicology

View full text Add to dashboard Cite

Freshwater zooplankton Daphnia magna has been widely used in ecotoxicology studies. During the last 20 years, it has been demonstrated that the topical application of juvenile hormone (JH) or JH analogs to mother daphnids induce male offspring production. Based on this finding, an in vivo screening validation method for chemicals with JH agonistic effect has developed. Although this screening system successfully identified a number of JH-like chemicals, molecular mechanisms underlying the male sex-determining process remain largely unknown. To address this issue, we established a reliable male-or female-producing system using Daphnia pulex WTN6 strain by changing the rearing photoperiod. Taking advantage of this rearing system, we successfully found several factors involving male sex determination such as ionotropic glutamate receptors, protein kinase C and pantothenate. Here, we used two D. magna strains that can also control the production of female or male offspring by photoperiod differences as model species for ecotoxicology studies. We demonstrated that either treatment of antagonist of ionotropic glutamate receptors or inhibitor of protein kinase C strongly suppressed male offspring production even under male-producing conditions. Moreover, we revealed that male sex-determining processes are likely diverged between D. magna and D. pulex based on the current experiment. This study provides a fine experimental method for in vivo screening not only JH agonists but also JH antagonists. Moreover, using daphnids with photoperiod-dependent sex determination manner will hugely contribute to understanding the mode-of-action of JH in daphnids.

show abstract

Section: Discussionmentioning

confidence: 99%

Methyl farnesoate regulatory mechanisms underlying photoperiod‐dependent sex determination in the freshwater crustacean Daphnia magna

Toyota

Satō

Iguchi

et al. 2020

J of Applied Toxicology

View full text Add to dashboard Cite

show abstract

“…Among 53 compounds with anti-JH activities, penfluridol showed high toxicity against larvae of the mosquito Aedes albopictus. Anti-JH activity increased in proportion to the concentration of penfluridol (Lee et al, 2018).…”

Section: Additional Anti-jh Compoundsmentioning

confidence: 95%

“…However, when these compounds are poorly applicable control agents, they are at least used as an effective tool in devising 'fourth generation insecticides' (Staal, 1986;Moya et al, 1997;Szczcpanik et al, 2005). Very recently, Lee et al (2018) reported that anti-JH compounds may be effective for controlling the target pests during their larval stages which have high level of endogenous JH titer.…”

Section: Potential Use Of Anti-jh Agents For Pest Controlmentioning

confidence: 99%