Mosquito (Diptera: Culicidae) fauna of the Iranian islands in the Persian Gulf

Azari-Hamidian, Shahyad; Linton, Yvonne‐Marie; Abai, Mohammad Reza; Ladonni, H.; Oshaghi, MA; Hanafi-Bojd, AA; Moosa-Kazemi, Seyed Hassan; Shabkhiz, Hossein; Pakari, A.; Harbach, Ralph E.

doi:10.1080/00222930903437358

Cited by 24 publications

(17 citation statements)

References 16 publications

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“…Mean pairwise (K2P) sequence diversity within An. calderoni was 0.70%, well within values of intraspecific variation reported for the barcoding region in other mosquito species (Cywinska et al 2006, Azari-Hamidian et al 2010. Haplotypes were unique by country, with mean intra-country pairwise distances of 0.04% and 0.77% within Colombian and Ecuadorian specimens, respectively, and an overall mean inter-country sequence divergence of 1.03%.…”

Section: Discussionsupporting

confidence: 76%

Confirmation of Anopheles (Anopheles) calderoni Wilkerson, 1991 (Diptera: Culicidae) in Colombia and Ecuador through molecular and morphological correlation with topotypic material

Obando

Carrejo

Wilkerson

et al. 2010

Mem. Inst. Oswaldo Cruz

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Section: Discussionsupporting

confidence: 76%

Confirmation of Anopheles (Anopheles) calderoni Wilkerson, 1991 (Diptera: Culicidae) in Colombia and Ecuador through molecular and morphological correlation with topotypic material

Obando

Carrejo

Wilkerson

et al. 2010

Mem. Inst. Oswaldo Cruz

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“…), the Persian Gulf region (Azari‐Hamidian et al . ) and China (Wang et al . ), showing the usefulness of DNA barcoding for Culicidae.…”

Section: Introductionmentioning

confidence: 99%

Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding

Versteirt

Nagy

Roelants

et al. 2014

Molecular Ecology Resources

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Since its introduction in 2003, DNA barcoding has proven to be a promising method for the identification of many taxa, including mosquitoes (Diptera: Culicidae). Many mosquito species are potential vectors of pathogens, and correct identification in all life stages is essential for effective mosquito monitoring and control. To use DNA barcoding for species identification, a reliable and comprehensive reference database of verified DNA sequences is required. Hence, DNA sequence diversity of mosquitoes in Belgium was assessed using a 658 bp fragment of the mitochondrial cytochrome oxidase I (COI) gene, and a reference data set was established. Most species appeared as well-supported clusters. Intraspecific Kimura 2-parameter (K2P) distances averaged 0.7%, and the maximum observed K2P distance was 6.2% for Aedes koreicus. A small overlap between intra- and interspecific K2P distances for congeneric sequences was observed. Overall, the identification success using best match and the best close match criteria were high, that is above 98%. No clear genetic division was found between the closely related species Aedes annulipes and Aedes cantans, which can be confused using morphological identification only. The members of the Anopheles maculipennis complex, that is Anopheles maculipennis s.s. and An. messeae, were weakly supported as monophyletic taxa. This study showed that DNA barcoding offers a reliable framework for mosquito species identification in Belgium except for some closely related species.

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“…In mosquitoes, the effectiveness of the COI barcode marker for specimen identification has been tested in surveys of Canada (Cywinska et al 2006), India (Kumar et al 2007), the Iranian islands in the Persian Gulf (Azari-Hamidian et al 2010), China (Wang et al 2012), Amazonian Ecuador (Linton et al 2013), Pakistan (Ashfaq et al 2014), Singapore (Chan et al 2014), and Belgium (Versteirt et al 2015). These studies show correspondence between morphological species and DNA barcode clusters, but also point out the failure of the methodology to distinguish between very similar or cryptic species.…”

Section: Introductionmentioning

confidence: 99%

Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology

Rozo-Lopez¹,

Mengual²

2015

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Colombia, one of the world’s megadiverse countries, has a highly diverse mosquito fauna and a high prevalence of mosquito-borne diseases. In order to provide relevant information about the diversity and taxonomy of mosquito species in Colombia and to test the usefulness of DNA barcodes, mosquito species collected at different elevations in the departments of Antioquia and Caldas were identified combining adult morphology and barcode sequences. A total of 22 mosquito species from eight genera were identified using these combined techniques. We generated 77 barcode sequences with 16 species submitted as new country records for public databases. We examined the usefulness of DNA barcodes to discriminate mosquito species from the Neotropics by compiling 1,292 sequences from a total of 133 species and using the tree-based methods of neighbor-joining and maximum likelihood. Both methodologies provided similar results by resolving 105 species of mosquitoes separated into distinct clusters. This study shows the importance of combining classic morphological methodologies with molecular tools to accurately identify mosquitoes from Colombia.

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Mosquito (Diptera: Culicidae) fauna of the Iranian islands in the Persian Gulf

Cited by 24 publications

References 16 publications

Confirmation of Anopheles (Anopheles) calderoni Wilkerson, 1991 (Diptera: Culicidae) in Colombia and Ecuador through molecular and morphological correlation with topotypic material

Confirmation of Anopheles (Anopheles) calderoni Wilkerson, 1991 (Diptera: Culicidae) in Colombia and Ecuador through molecular and morphological correlation with topotypic material

Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding

Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology

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