Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology

Rozo-Lopez, Paula; Mengual, Ximo

doi:10.3897/zookeys.513.9561

Cited by 39 publications

(29 citation statements)

References 91 publications

(101 reference statements)

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“…NJ trees using K2P substitution model are the most widely constructed phylogenetic trees used in many barcoding studies, especially on mosquitoes, to obtain more accurate recognition of the targeted group [3, 6, 9, 49]. Accordingly, the same methodology was applied during this study to increase the reliability of the results.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka

Weeraratne

Surendran

Reimer

et al. 2017

Malar J

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BackgroundGenus Anopheles is a major mosquito group of interest in Sri Lanka as it includes vectors of malaria and its members exist as species complexes. Taxonomy of the group is mainly based on morphological features, which are not conclusive and can be easily erased while handling the specimens. A combined effort, using morphology and DNA barcoding (using the markers cytochrome c oxidase subunit I (COI) gene and internal transcribed spacer 2 (ITS2) region, was made during the present study to recognize anophelines collected from eight districts of Sri Lanka for the first time.Methods Cytochrome c oxidase subunit I and ITS2 regions of morphologically identified anopheline mosquitoes from Sri Lanka were sequenced. These sequences together with GenBank sequences were used in phylogenetic tree construction and molecular characterization of mosquitoes.ResultsAccording to morphological identification, the field-collected adult mosquitoes belonged to 15 species, i.e., Anopheles aconitus, Anopheles annularis, Anopheles barbirostris, Anopheles culicifacies, Anopheles jamesii, Anopheles karwari, Anopheles maculatus, Anopheles nigerrimus, Anopheles pallidus, Anopheles peditaeniatus, Anopheles pseudojamesi, Anopheles subpictus, Anopheles tessellatus, Anopheles vagus, and Anopheles varuna. However, analysis of 123 COI sequences (445 bp) (16 clades supported by strong bootstrap value in the neighbour joining tree and inter-specific distances of >3%) showed that there are 16 distinct species. Identity of the morphologically identified species, except An. subpictus, was comparable with the DNA barcoding results. COI sequence analysis showed that morphologically identified An. subpictus is composed of two genetic entities: An. subpictus species A and species B (inter-specific K2P distance 0.128). All the four haplotypes of An. culicifacies discovered during the present study belonged to a single species. ITS2 sequences (542 bp) were obtained for all the species except for An. barbirostris, An. subpictus species B, An. tessellatus, and An. varuna. Each of these sequences was represented by a single species-specific haplotype.ConclusionsThe present study reflects the importance and feasibility of COI and ITS2 genetic markers in identifying anophelines and their sibling species, and the significance of integrated systematic approach in mosquito taxonomy. Wide distribution of malaria vectors in the country perhaps indicates the potential for re-emergence of malaria in the country.Electronic supplementary materialThe online version of this article (doi:10.1186/s12936-017-1876-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka

Weeraratne

Surendran

Reimer

et al. 2017

Malar J

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“…MEGA v 6.06 program (Tamura, Stecher, Peterson, Filipski, & Kumar, ) was used for sequences alignment, for searching for stop codons and for the calculation of intra‐ and interspecific genetic distances using the Kimura two parameters distance model (K2P) (Kimura, ; Tamura et al, ). To provide a graphic representation of the grouping pattern between the different species a K2P distance similarity tree was constructed using the Neighbor‐Joining model (NJ) with Bootstrap support values calculated with 1,000 replicates (Laurito et al, ; Rozo‐López & Mengual, ; Saitou & Nei, ).…”

Section: Methodsmentioning

confidence: 99%

“…Recent studies indicate that it is necessary to keep identifying mosquito species in different regions of the world through the use of traditional taxonomy and DNA sequences, to achieve a better estimation of mosquito biodiversity (Ashfaq et al, ; Azari‐Hamidian et al, ; Batovska, Blacket, Brown, & Lynch, ; Cywinska, Hunter, & Hebert, ; Rozo‐López & Mengual, ; Versteirt et al, ; Wang et al, ; Weeraratne, Surendran, & Parakrama Karunaratne, ). The combined use of DNA and morphology allowed to analyze a greater number of species worldwide and to discover cryptic speciation in taxa of medical importance such as culicides (Ashfaq et al, ; Hebert & Gregory, ; Laurito, Oliveira, Almirón, & Sallum, ; Torres‐Gutiérrez et al, ; Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

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DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Chan-Chablé

Martínez‐Arce

Mis-Ávila³

et al. 2019

Ecology and Evolution

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Culicidae mosquitoes are potential vectors of pathogens that affect human health. The correct species identification, as well as the discovery and description of cryptic species, is important in public health for the control and management of specific vectors. In the present study, the diversity of anthropophagous mosquitoes in Quintana Roo, at the border between Mexico and Belize, was evaluated using morphological and molecular data (COI‐DNA Barcoding). A total of 1,413 adult female specimens were collected, belonging to eight genera and 31 morphospecies. Most species formed well‐supported clades. Intraspecific Kimura 2 parameters (K2P) distance average was 0.75%, and a maximum distance of 4.40% was observed for Anopheles crucians s.l. ABGD method identified 28 entities, while 32 entities were identified with the BIN system. In Culex interrogator and Culex nigripalpus a low interspecific genetic distance of 0.1% was observed. One undescribed species belonging to the genus Aedes ( Aedes n. sp.) was discovered, but no clear genetic divergence was found between this species and the closely related species Aedes angustivittatus . An intraspecific K2P distance greater than 2.7% was observed in Aedes serratus (3.9%), Anopheles crucians s.l. (4.4%), Culex taeniopus (3.7%), Haemagogus equinus (3.9%), Culex erraticus (5.0%), Psorophora ferox (4.5%), and in Anopheles apicimacula (8.10%); therefore, evidences of cryptic diversity are shown in these species. This study showed that DNA barcodes offer a reliable framework for mosquito species identification in Quintana Roo, except for some closely related species for which it is recommended to use additional nuclear genetic markers such as ITS2, in order to resolve these small discrepancies.

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“…Standardized mitochondrial fragments from COI (DNA barcodes) have been used to identify mosquitoes (Cywinska et al 2006; Harrison et al 2012; Kumar et al 2007; Ruiz-Lopez et al 2012), including species from the Colombian Andes (Rozo-Lopez & Mengual 2015). Also, in Colombia, DNA barcodes were used to distinguish An.…”

Section: Introductionmentioning

confidence: 99%

Use of DNA barcoding to distinguish the malaria vector Anopheles neivai in Colombia

López-Rubio

Suaza-Vasco

Marcet

et al. 2016

Zootaxa

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A reference 535 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI), acquired from specimens of An. neivai Howard, Dyar & Knab, 1913 from its type locality in Panama, was used as a tool for distinguishing this species from others in the subgenus Kerteszia. Comparisons with corresponding regions of COI between An. neivai and other species in the subgenus (An. bellator Dyar & Knab 1906, An. homunculus Komp 1937, An cruzii Dyar & Knab, 1908 and An. laneanus Corrêa & Cerqueira, 1944) produced K2P genetic distances of 8.3–12.6%, values well above those associated with intraspecific variation. In contrast, genetic distances among 55 specimens from five municipalities in the Colombian Pacific coastal state of Chocó were all within the range of 0–2.5%, with an optimized barcode threshold of 1.3%, the limit for unambiguous differentiation of An. neivai. Among specimens from the Chocó region, 18 haplotypes were detected, two of which were widely distributed over the municipalities sampled. The barcode sequence permits discrimination of An. neivai from sympatric species and indicates genetic variability within the species; aspects key to malaria surveillance and control as well as defining geographic distribution and dispersion patterns.

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Mosquito species (Diptera, Culicidae) in three ecosystems from the Colombian Andes: identification through DNA barcoding and adult morphology

Cited by 39 publications

References 91 publications

Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka

Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Use of DNA barcoding to distinguish the malaria vector Anopheles neivai in Colombia

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