Mortality related to candidemia and risk factors associated with non-<i>Candida albicans</i>

Fagnani, Renata; Resende, Mariângela Ribeiro; Trabasso, Plı́nio; Mikami, Yuzuru; Schreiber, Angélica Zaninelli; Busso-Lopes, Ariane F.; Muraosa, Yasunori; Kamei, Katsuhiko; Moretti, Maria Luíza

doi:10.3109/23744235.2015.1075662

Cited by 2 publications

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“…Our study was not designed to include the clinical data of the patients for whom blood cultures were analyzed; however, by comparing the patients' clinical and epidemiologic findings, we observed a high mortality in patients with fungemia (55.7%). Previous studies performed in our hospital have also shown high mortality rates in patients with candidemia, ranging from 66% for non-intensive care unit (ICU) to 79% for ICU patients with candidemia (41). The high prevalence of patients with hematologic and nonhematologic cancers may reflect the referral population of our hospital, as described in previous studies (41,42).…”

Section: Discussionsupporting

confidence: 58%

Visible DNA Microarray System as an Adjunctive Molecular Test in Identification of Pathogenic Fungi Directly from a Blood Culture Bottle

et al. 2018

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A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridization, the results were visible with the naked eye. The performance of the microarray platform was evaluated against the commercial automated systems (Vitek 2 and BD Phoenix systems) and DNA sequencing (gold standard). A total of 461 blood culture bottles were tested: 127 positive for fungi, 302 positive for bacteria, and 32 that were negative. Once the microorganisms were identified by automated systems, fungal DNA was extracted directly from the blood culture bottles. The DNA products were tested using the microarray platform, and DNA sequencing was performed. The results of the microarray and DNA sequencing were concordant in 96.7% of cases, and the results from the automated systems and DNA sequencing were concordant in 98.4%. Of all the nucleotide sequences contained in the microarray platform, the microarray failed to identify four fungal isolates (one , two, and one ). Of note, the microarray detected DNA in two blood cultures from the same patient, whereas the automated system was only positive for Our microarray system provided reliable and fast fungal identification compared to that from DNA sequencing and the automated systems. The simplicity of reading the results by the naked eye made this DNA platform a suitable method for fungal molecular diagnosis.

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Section: Discussionsupporting

confidence: 58%