Morroniside promotes bone marrow mesenchymal stem cell proliferation in rats

Abstract

The aim of this study was to explore the mechanism underlying the promotive effect of morroniside on rat mesenchymal stem cell (RMSC) proliferation and to provide an experimental basis for the development of potential new drugs. RMSCs were obtained from the bone marrow of Sprague-Dawley rats aged 3-4 months. The proliferation of primary and subcultured RMSCs in the high‑, medium‑ and low‑concentration morroniside intervention and blank control groups was observed using light microscopy. Cell proliferation and survival conditions were detected using methyl thiazolyl tetrazolium (MTT) colorimetric tests. Light microscopy and the MTT assay revealed that RMSC adherence time in the morroniside groups was shorter compared with that of the control group. Twelve hours after the media of primary RMSCs were changed, the number of adherent cells in the morroniside groups increased and an elongated cell morphology was observed. The cells at the fourth passage in the morroniside groups fused completely 12‑16 h after inoculation and then rapidly entered into the logarithmic phase. The primary RMSCs of the morroniside intervention groups grew into typical bone marrow mesenchymal stem cell (BMSC) colonies after 4 days of morroniside treatment and their fusion rate had reached 80% after 9‑11 days. By contrast, the cell fusion rate of the control group only reached 75-80% after 14 days of morroniside treatment. Morroniside exhibited a similar promotive effect on proliferation in primary and subcultured RMSCs. Morroniside may promote RMSC proliferation through secreted factors, cell-to-cell interactions and/or the interactions between cellular adhesion molecules and extracellular matrices (ECMs). However, the specific mechanism underlying this effect remains to be fully elucidated.

Root bark of Sambucus Williamsii Hance promotes rat femoral fracture healing by the BMP-2/Runx2 signaling pathway

“…So we expected that one of the two components of EE-rbSWH may playing a mainly role on promoting osteoblasts differentiation, or the mutually coordinated actions of the two components. Morroniside, which was the highest content Iridoids compound in EE-rbSWH (more than 50%) (Han et al, 2013a(Han et al, , 2013b, also had the function of promoting osteogenic differentiation (Hu et al, 2013;Li et al, 2010). So we speculated it may be the main active ingredient of EE-rbSWH in promoting fracture healing.…”

Pharmacokinetics and Pharmacodynamics of Morroniside: A Review

“…The specific mechanism of morroniside promoting RMSC proliferation has yet to be clarified, but the process may occur by secreted factors, cell-to-cell interactions, and/or interactions between cellular adhesion molecules and extracellular matrices. 35 Manyu Li et al also found that morroniside isolated from Liuwei Dihuang pills may directly promote the differentiation and inhibit the apoptosis of MC3T3-E1 cells. The anti-osteoporotic mechanisms in MC3T3-E1 cells are likely to improve the activity of alkaline phosphatase, increase the contents of collagen type I and osteocalcin, downregulate the mRNA expression of caspase-3 and capase-9, and upregulated the mRNA expression of bcl-2.…”

Secondary Metabolites with Biomedical Applications from Plants of the Sarraceniaceae Family

“…It also prevents apoptosis of cardiomyocytes induced by high glucose [ 180 ]. In the skeletal system, morroniside promotes osteoblast proliferation [ 181 ] and formation through PI3K/Akt/mTOR signaling both in vitro and in vivo [ 182 ]. Morroniside activates Akt and extracellular signal-regulated kinase (ERK) to promote cartilage cell survival and matrix synthesis [ 183 ].…”