Morphotypic and molecular identification of filamentous fungi from Spanish blue-veined Cabrales cheese, and typing of Penicillium roqueforti and Geotrichum candidum isolates

Flórez, Ana Belén; Álvarez‐Martín, Pablo; López-Dı́az, Teresa-Marı́a; Mayo, Baltasar

doi:10.1016/j.idairyj.2006.04.002

Cited by 46 publications

(4 citation statements)

References 31 publications

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“…PCoA found the same grouping pattern as found in the cluster analysis, indicating that significant variation exists in this study, the separation of PCo1 and PCo2 showed that seven fungal isolates were dispersed in all quarters, indicating a high level of genotypic variation among the fungal isolates. These results were similar to Flórez et al (2007) who reported that genetic diversity found among the different P. roqueforti and G. candidum isolates was assessed by Rapid amplified polymeric DNA-polymerase chain reaction (RAPD-PCR) with two primers.…”

Section: Discussionsupporting

confidence: 90%

Morphological and Molecular Identification of Endophytic Fungi from Roots of Tomato and Evaluation of Their Antioxidant and Cytotoxic Activities

El-Debaiky¹,

El-Sayed²

2023

Egypt. J. Bot.

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E NDOPHYTIC fungi are identified as mitosporic and meiosporic ascomycetes that asymptomatically inhabit the internal tissues of some plants. They have a beneficial role in plant protection as more as producing several natural bioactive products. So, the present study aimed to isolate endophytic fungal species which associated with roots of tomato plants, sub-cultured in pure cultures and identified them according to their morphological and genetics characterization. The results introduced seven fungal species belong to the genera Alternaria, Aspergillus, Penicillium, Talaromyces and Triangularia which identified according to their cultural appearance and microscopic examination features. Their identification was confirmed by molecular characterization using sequencing the ITS and β-tubulin genes then deposited in GenBank with an accession numbers serial from OP599904.1 to OP599910.1 for ITS and from LC733668 to LC733674 for β-tubulin. Additionally, the antioxidant, antibacterial and antitumor studies of the crude filtrates of the isolated endophytic fungi revealed high antioxidant potential, negative antibacterial and successful antitumor activities.

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Section: Discussionsupporting

confidence: 90%

Morphological and Molecular Identification of Endophytic Fungi from Roots of Tomato and Evaluation of Their Antioxidant and Cytotoxic Activities

El-Debaiky¹,

El-Sayed²

2023

Egypt. J. Bot.

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“…On the other hand, the lowest values were achieved by A. nidulans and Geotrichum candidum being 7.09 and 13.71 mm. Florez et al [29] reported that, Geotrichum candidum is commonly found in mould-ripened cheese varieties. The strains consume lactate and produce several enzymes that break down proteins and fats.…”

Section: Efficiency Of the Fungal Strains For Proteolysismentioning

confidence: 99%

Role of Fungal Enzymes in the Biochemistry of Egyptian Ras Cheese during Ripening Period

El-Fadaly¹,

El-Kadi²,

Hamad³

et al. 2015

OALib

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“…The sequence of the ITS1-5.8S-ITS2 region appeared to be a credible molecular approach for fungal identification and discrimination [30]. The ITS region is able to differentiate and provide accurate and rapid identification of many fungi genera, such as Aspergillus, Fusarium, and Trichoderma at the species level [31].…”

Section: Molecular Identificationmentioning

confidence: 99%

Bioremediation of Crude Oil by Rhizosphere Fungal Isolates in the Presence of Silver Nanoparticles

Al-Zaban

Mahmoud

Alharbi

et al. 2020

IJERPH

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Background: This research work focuses on the utilization of indigenous fungi for in situ bioremediation of crude oil in the presence of silver nanoparticles. Methods: Two fungi belonging to two different genera showed promising crude oil-degrading abilities. Fungal isolates were identified based on internal transcribed spacer rDNA sequence analysis. Gas chromatography-mass spectrometry analysis of the crude oil remaining in the culture medium after seven days was performed. The response surface method (RSM) designed by Box-Behnken was used to establish a mathematical model. Inter-simple sequence repeat (ISSR) primers were used to examine the genetic variation of fungal isolates. Results: Gas chromatography-mass spectrometry (GC-MS) analysis after seven days showed that the optimum biodegradation of crude oil was 57.8%. The crude oil degradation rate was significantly affected by a temperature of 30 °C, pH value of 7, crude oil concentration of 4 g/L, a 1:1 ratio between A. flavus AF15 and T. harzianum TH07, and an silver nanoparticle (AgNP) concentration of 0.05 g. Molecular characterization in fungal isolates is extremely valuable when using ISSR markers. Conclusions: Two fungal isolates showed promising crude oil-degrading abilities with positive effect of low concentrations of AgNPs on biodegradation. RSM is an efficient mathematical method to optimize the microbial biodegradation of crude oil.

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Morphotypic and molecular identification of filamentous fungi from Spanish blue-veined Cabrales cheese, and typing of Penicillium roqueforti and Geotrichum candidum isolates

Cited by 46 publications

References 31 publications

Morphological and Molecular Identification of Endophytic Fungi from Roots of Tomato and Evaluation of Their Antioxidant and Cytotoxic Activities

Morphological and Molecular Identification of Endophytic Fungi from Roots of Tomato and Evaluation of Their Antioxidant and Cytotoxic Activities

Role of Fungal Enzymes in the Biochemistry of Egyptian Ras Cheese during Ripening Period

Bioremediation of Crude Oil by Rhizosphere Fungal Isolates in the Presence of Silver Nanoparticles

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