Bioremediation of Crude Oil by Rhizosphere Fungal Isolates in the Presence of Silver Nanoparticles

Al-Zaban, Mayasar I.; Mahmoud, Mohamed A.; Alharbi, Maha; Bahatheq, Aisha M

doi:10.3390/ijerph17186564

Cited by 17 publications

(5 citation statements)

References 38 publications

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“…The combinations were divided into four groups: The first group contained individual isolate, the second group contained two isolates, the third group contained three isolates, and the fourth group contained all four isolates. The initial inoculation quantity for each group was 5 mL of fungal suspension and 45 mL mineral salt medium (MSM) [ 17 ] of each set according to the principle of equal quantities ( Table 1 ). Initial, 45 mL of MSM in 100 mL of flasks was set with 1% crude oil.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of crude oil biodegradation using mixed fungal cultures

et al. 2021

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The use of potent fungal mixed cultures is a promising technique for the biodegradation of crude oil. Four isolates of fungi, namely, Alternaria alternata (AA-1), Aspergillus flavus (AF-3), Aspergillus terreus (AT-7), and Trichoderma harzianum (TH-5), were isolated from date palm soil in Saudi Arabia. The mixed fungal of the four isolates have a powerful tool for biodegradation up to 73.6% of crude oil (1%, w/v) in 14 days. The fungal consortium no. 15 containing the four isolates (1:1:1:1) performed significantly better as a biodegradation agent than other consortium in a variety of environmental factors containing crude oil concentration, incubation temperature, initial pH, biodegradation time and the salinity of the medium. The fungal consortium showed better performance in the biodegradation of normal alkanes (n-alkanes) than that of the polycyclic aromatic hydrocarbons (PAHs); the biodegradation efficiency of normal alkanes of the fungal consortium (67.1%) was clearly high than that of the PAHs (56.8%).

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Section: Methodsmentioning

confidence: 99%

Evaluation of crude oil biodegradation using mixed fungal cultures

et al. 2021

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“…The fungal isolate, Fusarium solani (FS12), was isolated and identified in a previous study (Al‐Zaban et al, 2020). This isolate was used in the present research as a feedstock for biodiesel output due to its potential capacity to accumulate and produce a high quantity of lipids in its biomass.…”

Section: Methodsmentioning

confidence: 99%

Production of biodiesel from oleaginous fungal lipid using highly catalytic bimetallic gold‐silver core‐shell nanoparticle

Al-Zaban

Alharbi

Mahmoud

et al. 2021

J of Applied Microbiology

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Aims: This study aims to synthesize, characterize and apply gold-silver core-shell nanoparticles (Au@Ag NPs), a nanocatalyst, to maximize biodiesel production from fungal isolate Fusarium solani (FS12) via a transesterification one-step reaction. Methods and Results:The Au@Ag NPs structure was examined by UV-vis spectrophotometer, transmission electron microscopy, X-ray diffraction and Fourier transform infrared (FTIR). All devices were used to characterize Au@Ag NPs and confirmed successful synthesis of nanoparticles. Fungal lipid was quantitatively determined by sulfo-phosphovanillin colorimetric method. Among 15 F. solani isolates obtained from rhizospheric soils of the date palm, F. solani (AF12) was chosen as the highly significant producer that accumulates above 20% lipid. The maximum biodiesel yield was 91.28 ± 0.19%, obtained under the optimum reaction conditions of 3% Au@Ag NPs as nanocatalyst concentration, and 1:20 oil to methanol molar ratio at 70℃ for 30 min. HPLC method was applied for monitoring in situ transesterification reaction. FTIR spectroscopy was used in qualitative analysis of biodiesel by verifying the presence of unique characteristic peaks of diagnostic significance. The quality of the biodiesel produced was confirmed by the high purity of fatty acid methyl esters analysis content up to >99%. Conclusions: These findings propose the applicability of F. solani (FS12) as a promising isolate to accumulate lipids and biodiesel production as a feedstock. Significance and Impact of the Study: The link between nanotechnology and fungi.Au@Ag NPs were synthesized at room temperature, which displayed high catalytic activity for in situ transesterification reaction. Catalytic activity appeared at low temperature, mole ratio and short reaction time. Oleaginous fungi are described as easily grown, have short life cycle, are cost-effective, and they utilized various sources of carbon up to waste and a simplified process to develop scale-up production as well, economic value, opposite the usage of vegetable oils which need for large agricultural areas.

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“…In a previous study, four fungal isolates were isolated: Aspergillus terreus KC462061, Aspergillus flavus (AF15), Trichoderma harzianum (TH07), and Fusarium solani (FS12). These isolates were identified morphologically and molecularly by internal transcribed spacer (ITS) regions according to the methodology of the previous study ( Al-Zaban et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Hydrocarbon biodegradation and transcriptome responses of cellulase, peroxidase, and laccase encoding genes inhabiting rhizospheric fungal isolates

Al-Zaban

Alharbi

Mahmoud

2021

Saudi Journal of Biological Sciences

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By using the indigenous micro-organisms of the polluted environment to be treated, bioremediation can be a successful strategy. PCR and RT-PCR molecular techniques were applied to examine the evolution of fungal isolates through putative genes f ligninolytic enzymes like lignin peroxidase (LiP), laccase (LaC), manganese peroxidase (MnP), and cellulase (Cx) as a response to polluting of the environment by hydrocarbons. In this study, isolation of rhizospheric fungal isolates, molecular identification, crude oil tolerance, and enzyme excretions were demonstrated. From the date palm rhizosphere, 3 fungal isolates were isolated and characterized morphologically and molecularly by ITS ribosomal RNA (rRNA) sequencing. The isolates were identified as Aspergillus flavus AF15, Trichoderma harzianum TH07, and Fusarium solani FS12 through using the BLAST tool in NCBI. All fungal isolates showed high tolerance to crude oil and survived with various responses at the highest concentration (20%). Aspergillus flavus AF15 and Trichoderma harzianum TH07 demonstrated promising oil-degrading tolerance ability based on the dose inhibition response percentage (DIRP) of the fungal isolates. A. flavus had a powerful capacity to production Cx, LaC, LiP and MnP with a range from 83.7 to 96.3 mL. Molecularly, nine genes of the ligninolytic enzymes, cbh ( cbhI.1 , cbhI.1 , cbhII ) lcc , lig (1, 2, 4 and 6) and mnp were tested for presence and expression (by PCR and RT-PCR, respectively). PCR showed that all isolates contained all the nine genes examined, regardless of capacity to enzymes production profiles, so the presence responses of nine genes did not correlate with enzymes-production ability. Gene expression analysis shows a more diverse pattern for tested isolates for example, Aspergillus flavus AF15 had over-expression of lig and mnp genes, Fusarium solani FS12 have a weak signal with lcc gene while, Trichoderma harzianum TH07 showed moderate expression of mnp and lcc genes. The power of the transcription of the gene leads to increased enzyme secretion by fungal isolates. Fungi are important microorganisms in the clean-up of petroleum pollution. They have bioremediation highly potency that is related to their diverse production of these catalytic enzymes.

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Bioremediation of Crude Oil by Rhizosphere Fungal Isolates in the Presence of Silver Nanoparticles

Cited by 17 publications

References 38 publications

Evaluation of crude oil biodegradation using mixed fungal cultures

Evaluation of crude oil biodegradation using mixed fungal cultures

Production of biodiesel from oleaginous fungal lipid using highly catalytic bimetallic gold‐silver core‐shell nanoparticle

Hydrocarbon biodegradation and transcriptome responses of cellulase, peroxidase, and laccase encoding genes inhabiting rhizospheric fungal isolates

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