Morphometrical and molecular data on plant parasitic nematodes <i>Longidorus attenuatus</i> Hooper, 1961 and <i>L. danuvii</i> Barsi et al., 2007 (Nematoda: Longidoridae) reported from Ukraine for the first time

A 7-year study was conducted to determine nematode species belonging to the Longidoridae and Trichodoridae families in agricultural areas in Thrace, Turkey. For this purpose, soil samples were collected from fruit, vegetable, vineyard, and forest areas. Eleven nematode species belonging to Xiphinema, Longidorus, and Trichodorus were recovered from soils around the rhizosphere of 28 plants. Identified species include X. pachtaicum (rhizosphere of 26 plants, e.g., grapevine and olive ), X. turcicum (rhizosphere of grapevine), X. pyrenaicum (rhizosphere of grapevine and fig), X.ingens (rhizosphere of grapevine), X. italiae (rhizosphere of, e.g., grapevine and fig), X. index (habitat of nine plants, e.g., olive, peach), Xiphinema diversicaudatum (rhizosphere of grapevine and fig), X. opisthohysterum (rhizosphere of grapevine), L. elongatus (rhizosphere of four plants, e.g., kiwi and cherry), L. attenuatus (habitat of olive and grapevine) and T. similis (rhizosphere of grapevine and walnut). Of all plants, the habitat of 22 constitutes a new record for longidorid and trichodorid nematodes in Turkey. This article also includes information generated on a national scale for Xiphinema spp., Longidorus spp., and Trichodorus spp. being identified in Turkey.

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First Reports, Morphological, and Molecular Characterization of Longidorus caespiticola and Longidorus poessneckensis (Nematoda: Longidoridae) from Ukraine

Susulovska

Castillo

Archidona-Yuste

2017

Journal of Nematology

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Seven needle nematode species of the genus Longidorus have been reported in Ukraine. Nematological surveys for needle nematodes were carried out in Ukraine between 2016 and 2017 and two nematode species of Longidorus (L. caespiticola and L. poessneckensis) were collected from natural and anthropogenically altered habitats on the territory of Opillia and Zakarpattia in Ukraine. Nematodes were extracted from 500 cm 3 of soil by modified sieving and decanting method. Extracted specimens were processed to glycerol and mounted on permanent slides and subsequently identified morphologically and molecularly. Nematode DNA was extracted from single individuals and PCR assays were conducted as previously described for D2-D3 expansion segments of 28S rRNA. Sequence alignments for D2-D3 from L. caespiticola showed 97%-99% similarity to other sequences of L. caespiticola deposited in GenBank from Belgium, Bulgaria, Czech Republic, Russia, Slovenia, and Scotland. Similarly, D2-D3 sequence alignments from L. poessneckensis, showed 99% to other sequences of L. poessneckensis deposited in GenBank from Slovakia and Czech Republic. Morphology, morphometry, and molecular data obtained from these samples were consistent with L. caespiticola and L. poessneckensis identification. To our knowledge, these are the first reports of L. caespiticola and L. poessneckensis in Ukraine, extending the geographical distribution of these species.

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Morphometrical and molecular data on plant parasitic nematodes Longidorus attenuatus Hooper, 1961 and L. danuvii Barsi et al., 2007 (Nematoda: Longidoridae) reported from Ukraine for the first time

Cited by 3 publications

References 11 publications

Longidorus attenuatus (needle nematode)

Longidorus attenuatus (needle nematode)

Türkiye’de Longidoridae ve Trichodoridae familyalarına bağlı nematod türlerinin konukçularına ilişkin yeni veriler

First Reports, Morphological, and Molecular Characterization of Longidorus caespiticola and Longidorus poessneckensis (Nematoda: Longidoridae) from Ukraine

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