First Reports, Morphological, and Molecular Characterization of Longidorus caespiticola and Longidorus poessneckensis (Nematoda: Longidoridae) from Ukraine

Abstract

Seven needle nematode species of the genus Longidorus have been reported in Ukraine. Nematological surveys for needle nematodes were carried out in Ukraine between 2016 and 2017 and two nematode species of Longidorus (L. caespiticola and L. poessneckensis) were collected from natural and anthropogenically altered habitats on the territory of Opillia and Zakarpattia in Ukraine. Nematodes were extracted from 500 cm 3 of soil by modified sieving and decanting method. Extracted specimens were processed to glycerol and mounted on permanent slides and subsequently identified morphologically and molecularly. Nematode DNA was extracted from single individuals and PCR assays were conducted as previously described for D2-D3 expansion segments of 28S rRNA. Sequence alignments for D2-D3 from L. caespiticola showed 97%-99% similarity to other sequences of L. caespiticola deposited in GenBank from Belgium, Bulgaria, Czech Republic, Russia, Slovenia, and Scotland. Similarly, D2-D3 sequence alignments from L. poessneckensis, showed 99% to other sequences of L. poessneckensis deposited in GenBank from Slovakia and Czech Republic. Morphology, morphometry, and molecular data obtained from these samples were consistent with L. caespiticola and L. poessneckensis identification. To our knowledge, these are the first reports of L. caespiticola and L. poessneckensis in Ukraine, extending the geographical distribution of these species.

First report with morphometrics and molecular characterization of phtyonematodes associating mango trees in the tropics of Saudi Arabia

“…The use of modern molecular tools and the phylogenetic analysis offer a good solution for the misleading problems of nematode identification by the classical methods. The large subunit of the nuclear ribosomal RNA (28S rRNA), also known as LSU-rRNA has conserved domains and certain expansion segments such as D2-D3 expansion region which is very useful for the molecular characterization of many nematode species (Bae et al, 2010, Susulovska et al, 2017). However, D2 segment is more variable, compared to D3 segment (Douda et al, 2013).…”

Morphological and chorological characterization of Longidorus intermedius Kozlowska & Seinhorst, 1979 firstly reported from Ukraine with the comments on Longidorus elongatus (de Man, 1876) Thorne & Swanger, 1936

“…Nine Longidorus species have been previously reported from Ukraine: Longidorus attenuatus Hooper, 1961, L. caespiticola Hooper, 1961, L. danuvii Barsi Thorne, 1939. [11,13,14]. During recent survey, four populations of Longidorus genus were identified as Longidorus intermedius Kozlovska, Seinhorst, 1979.…”

“…Oesophago-intestinal valve variable in shape, from conical to rounded. Vuvla a transverse slit situated almost equatorially, vagina 16(13)(14)(15)(16)(17)(18)(19)) µm wide and 26 (23-33) µm long occupying approximately half of the corresponding body width. Reproductive system amphidelphic, with equally developed…”