Morphometric evaluation of seminiferous tubule and proportionate numerical analysis of Sertoli and spermatogenic cells indicate differences between crossbred and purebred bulls

Abstract

Aim:The present study compared the testicular cytology and histology between crossbred (Holstein–Friesian [HF] × Tharparkar) and purebred (HF and Tharparkar) bulls to find out differences if any.Materials and Methods:Four peripubertal bulls from each breed were utilized for the study. Through percutaneous needle aspiration biopsy, Sertoli and spermatogenic cells were extracted, and morphometry was studied. For histological studies, testicular tissues obtained through unilateral castration were utilized. Sertoli cells specific GATA4 antibody was used to study the population of Sertoli cells in the seminiferous tubule through immunofluorescence.Results:The testicular weight, volume, and scrotal circumference differed significantly among the breeds. The diameter and area of the seminiferous tubule was high in HF, followed by Karan Fries (KF), and Tharparkar bulls. However, the degree of compactness, based on qualitative evaluation, was high in Tharparkar followed by KF and HF bulls. The intensity of Leydig cells was higher in Tharparkar bulls followed by KF and HF. The proportion of Sertoli cells was higher (p<0.05) in HF and Tharparkar bulls compared to KF bulls.Conclusion:It may be concluded that variations exist in testicular components of the breeds studied and the proportion of Sertoli cells in relation to spermatogenic cells was significantly lower in crossbred bulls compared to purebred bulls.

Effects of a standardized 40% ellagic acid pomegranate (Punica granatum L.) extract on seminiferous tubule histopathology, diameter, and epithelium thickness in albino Wistar rats after heat exposure

“…The resulting prevention of the damage of cell membranes and preservation of hormone receptors involved in the promotion of spermatogenesis has been shown to result in an increase in spermatogenic cells [10]. Increase in the spermatogenic and Sertoli cells in the tubular epithelium results in narrowing of seminiferous tubule lumen, thickening of seminiferous epithelium, and increase the diameter of the seminiferous tubule [11]. According to Franca and Russell [12], there is a positive relation between tubular diameter and the spermatogenic activity.…”

A High Phosphorus Diet Impairs Testicular Function and Spermatogenesis in Male Mice with Chronic Kidney Disease

“…Testicular seminiferous parameters such as the thickness of the germinal epithelium, mean seminiferous tubule diameter (MSTD), and testicular spermatogenesis were measured. The thickness of the germinal epithelium and diameter of the seminiferous tubule were calculated across the horizontal and vertical axes using ImageJ software (1.50, National Institutes of Health, MD, USA) [ 24 ]. The Johnsen score was used to determine testicular spermatogenesis in each group, judging from the level of sperm maturation and scoring from 1 to 10 [ 25 ].…”

Effect of co-administration ofHypoxis hemerocallideaextract and antiretroviral therapy (HAART) on the histomorphology and seminal parameters in Sprague Dawley rats

“…For the light microscopic studies, testes fixed in neutral buffered formalin (NBF) were dehydrated in various grades of ethanol, cleared in xylene and finally embedded in paraffin wax blocks as previously performed in Tripathi et al. () with slight modifications (while Bouin's fluid and benzene were used as fixative and clearing agent, we used NBF and Xylene as fixative and clearing agent respectively). Sections (5 μm) prepared using rotary microtome and mounted on albumenized glass slides were stained by haematoxylin and eosin for light microscopy.…”