Morphometric Characteristics of Rat Salivary Glands Hemomicrovasculature Capacity Component Under Normal Conditions and in Ethanol Chronic Intoxication

Єрошенко, Галина Анатоліївна; Ерошенко, Галина Анатольевна; Yeroshenko, G. А.; Шевченко, К. В.; Шевченко, К. В.; Якушко, Олена Святославівна; Якушко, Елена Святославовна; Yakushko, O. S.

doi:10.26724/2079-8334-2018-3-65-149-152

Cited by 13 publications

(5 citation statements)

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“…The first group consisted of intact rats, which were under normal conditions without the influence of additional factors. The second group of animals was simulated alcohol intoxication by administering 40% ethanol solution at a rate of 12 mg/kg body weight intragastrically 4 times a day [19,25,26]. On day 30 after the start of the experiment, rats were euthanized with thiopental anesthesia at a rate of 25 mg/kg body weight.…”

Section: Methodsmentioning

confidence: 99%

Morphometric features of rat pinealocytes in conditions of chronic ethanol intoxication

Pshychenko

Черно

2021

Rep. of Morph.

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Ethanol has chronobiological effects, which are associated with inhibition of melatonin synthesis and secretion and disruption of the sleep-wake cycle. Ethanol is known to cause sleep fragmentation due to frequent awakenings, prolong wakefulness, and reduce the duration of the slow sleep phase. At the same time, changes in the morphology of the pineal gland under chronic exposure to ethanol remain poorly studied. Of particular interest are changes in the basic morphometric parameters of pinealocytes, because they are a marker of the functional state of the pineal gland. The aim of the study was to morphometrically study the features of morphological changes in rat pinealocytes in the physiological norm and in chronic ethanol intoxication. To achieve this goal, we used 20 laboratory male rats: a control group and an experimental group. The control group was under standard vivarium conditions. For the experimental group, alcohol intoxication was modeled by injecting a 40% ethanol solution at the rate of 12 mg/kg of body weight intragastric 4 times a day. The morphometric parameters of pinealocytes were studied on day 30 from the start of the study. According to the results of morphometric measurements, a significant increase in the parameters of light pinealocytes and a decrease in the parameters of dark cells were established. It was determined that the average values of the cytoplasm area of light pinealocytes increase by 54.55% (p<0.05), the area of the nucleus increases by 61.32% (p<0.05), and the area of the nucleolus by 32.84% (p<0.05) compared with the control group. The area of the cytoplasm of dark pinealocytes decreases by 27.2% (p<0.05), and the area of the nucleus by 37.33% (p<0.05). Changes in the ratio of light and dark pinealocytes were also noted. An increase in the number of active light cells by 8.17% was found. The detected morphometric changes indicate high functional activity of cells, which has a compensatory nature in response to apoptosis of pinealocytes.

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Section: Methodsmentioning

confidence: 99%

Morphometric features of rat pinealocytes in conditions of chronic ethanol intoxication

Pshychenko

Черно

2021

Rep. of Morph.

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“…10 animals were assigned in the control group and were administered with isotonic saline solution QID, delivered directly into the stomach. 40 animals were assigned in the experimental group, who were administered with 12 mg/kg ethanol 40 о QID, delivered directly into the stomach [16]. Animals were withdrawn from the experiment on day 5, 9, 12 and 30 by thiopental anesthesia overdose (25 mg / kg).…”

Section: Methodsmentioning

confidence: 99%

Remodeling of the Duct System of the Rat Submandibular Salivary Glands in Chronic Ethanol Intoxication

et al. 2020

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The aim: To determine the dynamics of changes in metric parameters of the duct system of rat submandibular glands in normal conditions and chronic ethanol intoxication. Materials and methods: 50 albino outbred rats were involved in the experiment. 10 animals were assigned in the control group, 40 animals – in the experimental group. Animals were sacrificed on 5, 9, 12 and 30 days by overdose of thiopental anesthesia. Pieces of the submandibular glands were embedded into epon-812 according to the conventional technique. Results: On day 5 of the experiment the lumen diameter of intercalated duct reduced by 9,15 % (р <0,05). The lumen diameter of the striated ducts was by 5,29 % significantly greater than the values in controls (р <0,05). The lumen diameter of the granular ducts reduced by 2,45 % (р <0,05). On day 30 of the experiment the height of the epithelial cells of the intercalated ducts was by 8,47 % significantly less (р <0,05), the height of the epithelial cells of the striated ducts was by 12,27 % less (р <0,05) and the height of the epithelial cells of the granular ducts was by 11,96 % less (р <0,05) than the values in controls. Conclusions: No recovery of parameters occurs by day 30 of the experiment, indicating the depletion of the secretory epithelium of the duct system, due to dystrophic changes caused by vascular disorder in the microvasculature.

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“…Алкогольне ураження СЗ відтворювали шляхом внутрішньошлункового (через зонд) введення 40%-го розчину етанолу в дозі 24 мг/кг 2 рази на день протягом 14 діб [14].…”

Section: вплив поліфенолів на пероксидне окиснення ліпідів та антиокс...unclassified

The Effect of Polyphenols on Lipid Peroxidation and the Antioxidant System in the Submandular Salivary Glands during Combined Administration of Alcohol and S. typhi Lipopolysaccharide

Kozaeva¹,

Klymenko²

2022

Ukr. ž. med. bìol. sportu

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The purpose of the study was to study the effect of polyphenols (curcumin, epigallocatechin-3-gallate, and quercetin) on indicators of lipid peroxidation and antioxidant protection in the submandibular salivary glands of rats with alcohol damage against the background of lipopolysaccharide-induced systemic inflammatory response. Materials and methods. The studies were conducted on 35 rats of the Wistar line weighing 205–220 g, divided into 5 groups of seven animals in each: the 1st group (control) included animals receiving isotonic sodium chloride solution intragastrically twice a day; the 2nd group included rats exposed to alcohol (in a dose of 24 mg/kg intragastrically through gavage twice a day) for last 2 weeks during lipopolysaccharide-induced systemic inflammatory response; the rats of the 3rd, 4th and 5th groups were exposed to alcohol during lipopolysaccharide-induced systemic inflammatory response, which also received polyphenols, administered intragastrically: curcumin (diferuloylmethane, in a daily dose of 200 mg/kg), epigallocatechin-3-gallate (in a daily dose of 40 mg/kg), quercetin (in a daily dose of 200 mg/kg), respectively. The level of lipid peroxidation in the tissues of the submandibular salivary glands was assessed by the formation of compounds that react with thiobarbituric acid. The state of antioxidant protection was assessed by the increase in the concentration of thiobarbituric acid-active products during a 1.5-hour incubation in iron ascorbate buffer solution, as well as by the activity of superoxide dismutase. Results and discussion. When using curcumin under the experimental conditions, the concentration of thiobarbituric acid-reactants in the homogenate of submandibular salivary glands before and after incubation was 59.3 and 55.7% inferior to the corresponding results of the 2nd group, when prescribing epigallocatechin-3-gallate – by 55.7 and 51.2%, quercetin – by 67.0 and 61.0%, respectively. This was accompanied by a probable increase in the activity of superoxide dismutase in the tissues of the submandibular salivary glands. Conclusion. The use of curcumin and bioflavonoids (epigallocatechin-3-gallate and quercetin) under conditions of combined administration of 40% ethanol and S. typhi lipopolysaccharide significantly limits the development of lipid peroxidation in the tissues of the submandibular salivary glands, increases their antioxidant potential, superoxide dismutase activity

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Morphometric Characteristics of Rat Salivary Glands Hemomicrovasculature Capacity Component Under Normal Conditions and in Ethanol Chronic Intoxication

Cited by 13 publications

References 0 publications

Morphometric features of rat pinealocytes in conditions of chronic ethanol intoxication

Morphometric features of rat pinealocytes in conditions of chronic ethanol intoxication

Remodeling of the Duct System of the Rat Submandibular Salivary Glands in Chronic Ethanol Intoxication

The Effect of Polyphenols on Lipid Peroxidation and the Antioxidant System in the Submandular Salivary Glands during Combined Administration of Alcohol and S. typhi Lipopolysaccharide

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