Morphometric allometry of representatives of three naviculoid genera throughout their life cycle

Woodard, K. E.; Kulichová, Jana; Poláčková, Tereza; Neustupa, Jiří

doi:10.1080/0269249x.2016.1227375

Cited by 13 publications

(24 citation statements)

References 34 publications

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“…Cell size declined markedly during the vegetative phase of the life cycle, and strong allometric effect caused profound shape differences between big and small cells [23]. This pattern was reflected in our Procrustes ANOVA models by components of symmetric variation among the cells (factor "individual") that revealed highly significant variability in both populations.…”

Section: Discussionsupporting

confidence: 52%

“…These analyses of total asymmetry could be based on PCA of the Procrustes-aligned configurations that form the symmetry group of the biradial object under study [6,12,39]. Specifically, a variant of this method that includes separate PCAs of the symmetry group for each cell would allow straightforward comparison of the datasets with different numbers of cells [23], an analysis closely related to the continuous symmetry measure introduced by Zabrodsky et al [11,40,41].…”

Section: Discussionmentioning

confidence: 99%

“…Both strains were cultivated for over a year to capture the entire size range of the cells throughout the life cycle, as described in our previous study [23]. The strains were cultivated in liquid WC medium [28] at 18 • C in 7 cm diameter Petri dishes and illuminated at 40 µmol·m −2 ·s −1 by 18-W cool fluorescent tubes (Phillips TLD 18W/33, Amsterdam, The Netherlands).…”

Section: Culturing and Data Acquisitionmentioning

confidence: 99%

“…The medium was exchanged weekly to avoid nutrient depletion, and approximately 10% of the cells were transplanted to new Petri dishes monthly in order to avoid overcrowding. Samples were collected from the cultures and processed for light microscopy analysis every second month [23]. Permanent slides were made from the samples [27,29], with micrographs of 50 valves captured from each permanent slide (i.e., a sample corresponding to a certain life-cycle stage in a culture) using an Olympus BX-51 light microscope (Olympus Corporation, Tokyo, Japan) fitted with an Olympus Z5060 digital camera.…”

Section: Culturing and Data Acquisitionmentioning

confidence: 99%

“…Shape variation of pennate diatoms is predominantly determined by allometric changes relating to size diminution of frustules [19,23], and therefore, we also examined the relationship between the DA and FA of valve outlines across the longitudinal axis and cell size. Does FA (or even DA) increase during successive vegetative divisions, thereby resulting in accumulating irregularities in the shape of the siliceous frustules?…”

Section: Introductionmentioning

confidence: 99%

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Morphometric Asymmetry of Frustule Outlines in the Pennate Diatom Luticola poulickovae (Bacillariophyceae)

Woodard

Neustupa

2016

Symmetry

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Abstract:Side orientation of cells is usually ambiguous in unicellular organisms, making it impossible to separate components of directional asymmetry (DA) and fluctuating asymmetry (FA). However, frustules of the diatom Luticola poulickovae have biradially symmetric outlines, and their central areas bear ornamentation that is asymmetric across the apical axis. The goal of this study was to explore differentiation of morphometric asymmetry across the apical axis into DA and FA components. Is there detectable DA of the valve outlines of two L. poulickovae strains that may be related to the asymmetric central areas? Given that the life cycle of diatoms involves cell-size diminution, and cell shape is strongly affected by allometry, we also explored the question of whether asymmetry is correlated with cell size. The extent of symmetric variation among individuals in each strain, as well as DA and FA across the apical axis, were quantified using two Procrustes ANOVA models. The results revealed no correlation of either total asymmetry or FA with valve size. DA was significant and considerably more pronounced than FA in both strains, indicating that there is previously unknown systematic asymmetry of valve outlines of L. poulickovae, which may be related to the asymmetry of its central area.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Culturing and Data Acquisitionmentioning

confidence: 99%

Section: Culturing and Data Acquisitionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphometric Asymmetry of Frustule Outlines in the Pennate Diatom Luticola poulickovae (Bacillariophyceae)

Woodard

Neustupa

2016

Symmetry

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Exploring the effects of acid mine drainage on diatom teratology using geometric morphometry

et al. 2017

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Metal pollution of aquatic habitats is a major and persistent environmental problem. Acid mine drainage (AMD) affects lotic systems in numerous and interactive ways. In the present work, a mining area (Roșia Montană) was chosen as study site, and we focused on two aims: (i) to find the set of environmental predictors leading to the appearance of the abnormal diatom individuals in the study area and (ii) to assess the relationship between the degree of valve outline deformation and AMD-derived pollution. In this context, morphological differences between populations of Achnanthidium minutissimum and A. macrocephalum, including normal and abnormal individuals, were evidenced by means of valve shape analysis. Geometric morphometry managed to capture and discriminate normal and abnormal individuals. Multivariate analyses (NMDS, PLS) separated the four populations of the two species mentioned and revealed the main physico-chemical parameters that influenced valve deformation in this context, namely conductivity, Zn, and Cu. ANOSIM test evidenced the presence of statistically significant differences between normal and abnormal individuals within both chosen Achnanthidium taxa. In order to determine the relative contribution of each of the measured physico-chemical parameters in the observed valve outline deformations, a PLS was conducted, confirming the results of the NMDS. The presence of deformed individuals in the study area can be attributed to the fact that the diatom communities were strongly affected by AMD released from old mining works and waste rock deposits.

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Mini review: Diatom species as seen through a molecular window

Medlin

2018

Braz. J. Bot

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It has been accepted that we know less than 10% of the identified diversity in the marine microbial world and the diatoms are no exception. Even the species that we think we can easily recognize are often cryptic species, and even less is known of their life histories and spatial and temporal trends in their abundance and distribution. With new molecular and analytical techniques, we can advance our knowledge of a species to understand its morphological range, biogeographies and reproductive isolation. Moreover, some of molecular techniques are very sensitive. Depending on the species-level question(s) being asked, the molecular tools appropriate to answer them differ greatly.

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Morphometric allometry of representatives of three naviculoid genera throughout their life cycle

Cited by 13 publications

References 34 publications

Morphometric Asymmetry of Frustule Outlines in the Pennate Diatom Luticola poulickovae (Bacillariophyceae)

Morphometric Asymmetry of Frustule Outlines in the Pennate Diatom Luticola poulickovae (Bacillariophyceae)

Exploring the effects of acid mine drainage on diatom teratology using geometric morphometry

Mini review: Diatom species as seen through a molecular window

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