Morphology of the reproductive tract of females of leaf beetle Chrysomela populi (Chrysomelidae: Coleoptera)

Abstract

In this study, oocyte development and vitellogenesis of the leaf beetle Chrysomela populi are described and illustrated using light and scanning electron microscopies. The female reproductive tract of C. populi consists of a pair of ovaries, a pair of lateral oviducts, a common oviduct and a spermathecae. Each ovary has 14 telotrophic meroistic ovarioles. Each ovariole consists of a terminal filament, germarium, vitellarium and pedicel. Terminal filament is thin, long thread shaped. The germarium contains trophocytes towards the distal part, prefollicular cells and oocytes towards the proximal part in the young stage. The vitellarium contains 3-4 oocytes at different stages of development (previteloogenic, vitellogenic and choriogenic oocytes). Previtellogenic oocytes located towards the distal end of the vitellarium are surrounded by follicle epithelium. Vitellogenic oocytes are surrounded by a monolayer follicular epithelium, and their ooplasma is basophilic. The ooplasma of the oocytes in the choriogenic stage, which is the last stage of vitellogenesis, is filled with eosinophilic yolk granules of different sizes. Chorion is clearly differentiated between the vitelline membrane surrounding the ooplasma and the follicular epithelium. The pedicel connects the ovarioles with the lateral oviduct. A pair of lateral oviducts are connected to the common oviduct. A spermatheca is located towards the proximal part of the common oviduct. Spermatheca consists of a reservoir, duct and gland. The eggs are light yellow in color and the chorion is very thin. This study intends to contribute to female reproductive biology studies in Coleoptera and other insect order species, agricultural struggle studies in terms of researching C. populi.

The Female Reproductive System Structure of Isotomus speciosus (Schneider, 1787) (Coleoptera: Cerambycidae) and Mylabris cernyi Pan & Bologna, 2014 (Coleoptera: Meloidae)-Light and Scanning Electron Microscopy Studies

“…For example, since the ovarioles are in the same plane in I. speciosus, the terminal lament connecting the ovarioles is of equal length, but in M. cernyi, the terminal lament lengths differ because the ovarioles are in different planes. Additionally, looking at the thickness of the terminal lament, it is distinguished that the terminal lament of I. speciosus is twice as thick as that of M. cernyi and C. populi (Chrysomelidae) (Özyurt Koçakoğlu et al, 2021). In the germarium, the second part of the ovariole, trophocytes of both species are distinguished, but the germarium wide varied interspeci cally.…”

“…In the germarium, the second part of the ovariole, trophocytes of both species are distinguished, but the germarium wide varied interspeci cally. The germarium wide of I. speciosus (~ 0.17 mm wide) is larger than that of M. cernyi and C. populi (Chrysomelidae) (~ 0.1 mm wide) (Özyurt Koçakoğlu et al, 2021). In both species, previtellogenesis, vitellogenesis, and choriogenesis stages oocytes are found in the vitellarium, the third part of the ovariole, as in C. populi (Chrysomelidae) and C. herbacea (Chrysomelidae) (Özyurt Koçakoğlu et al, 2021Koçakoğlu et al, , 2022a.…”

“…In M. cernyi and I. speciosus, a spermatheca and a bursa copulatrix are seen around the common oviduct, as in Capnodis tenebrionis (Linnaeus, 1761) (Buprestidae) (Özyurt Koçakoğlu et al, 2022b). However, some species have only spermatheca and there is no bursa copulatrix, as in Tenuisvalvae notata (Mulsant, 1850) (Coccinellidae), C. populi (Chrysomelidae), and C. herbacea (Chrysomelidae) (Túler et al 2018;Özyurt Koçakoğlu et al, 2021Özyurt Koçakoğlu et al, , 2022a.…”

“…M.cernyi has a large bulging, sac-like shaped, white bursa copulatrix and a cylindrical sac shaped and white spermatheca. In C. populi (Chrysomelidae) and C. herbacea (Chrysomelidae), the spermatheca has brown and hook -shaped (Özyurt Koçakoğlu et al, 2021Koçakoğlu et al, , 2022a. The spermatheca of T. notata (Coccinellidae) is round or rhiniform and brown (Túler et al 2018).…”

Morphology of female reproductive system of Mediterranean flatheaded peachborer, Capnodis tenebrionis (Linnaeus, 1761) (Coleoptera: Buprestidae)

“…Similar structures were also seen in most coleopteran species (El Naggar et al, 2010; Gardner, 1986; Malekpour et al, 2010; Nasserzadeh et al, 2005; Stringer, 1988). However, bursa copulatrix is not seen in the female reproductive system of Phaedon brassicae Baly (Chrysomelidae), Chrysomela populi Linnaeus, 1758 (Chrysomelidae), and Chrysolina herbacea (Duftschmid 1825) (Chrysomelidae) (Özyurt Koçakoğlu et al, 2021a, 2021b; Wang et al, 2007). The similarities and differences between insects depend on the phylogeny of the species.…”