Morphology of the Pineal Glin Pregnant Nursing Sows

Wyrzykowski, Z; Przybylska, B; Wyrzykowska, K

doi:10.1111/j.1600-079x.1987.tb00849.x

Cited by 10 publications

(7 citation statements)

References 19 publications

(5 reference statements)

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“…Morphologically, the pineal gland of the pig is characterized by a large number of electron-dense bodies in pinealocyte (Karasek and Wyrzykowski, 1980;. The structure and number of the bodies change under various physiological (Wyrzykowski et al, 1987;Przybylska, 1990) and experimental (Przybylska et al, 1993 conditions.…”

Section: Tion; Pigmentioning

confidence: 99%

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Przybylska‐Gornowicz¹,

Lewczuk²,

Møller

1997

Anat. Rec.

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Background The pineal gland is richly innervated by the neuropeptide Y (NPY)‐positive nerves that mostly exibit immunoreactivity for the enzyme tyrosine hydroxylase, a marker of sympathetic nerve fibers. NPY is synthesized as a part of larger prepromolecule. The present study was undertaken to demonstrate that the posttranslational processing of preproNPY resulted in the presence of C‐terminal flanking peptide of NPY (CPON) in nerve fibers of the pig pineal gland. Methods An immunohistochemical anti‐CPON technique was done over mounted sections of perfusion‐paraformaldehyde‐fixed material. An immunocytochemical preembedding technique was done to study the CPON‐positive nerve terminals under electron microscopy. Results The pig pineal gland is densely innervated by CPON‐immunoreactive nerve fibers. These nerve fibers follow from the pineal capsule into the connective tissue septa and farther into the pineal parenchyma, where the varicose branches terminate between the pinealocytes. The fiber density was the highest in the peripheral and ventral parts of the gland. At the ultrastructural level, the CPON‐immunoreactive nerve terminals were found in the perivascular spaces and in the parenchyma. The terminals contained small vesicles (30‐40 nm in diameter), some of which showed an eccentrically located dense core. In addition, large clear vesicles (80‐100 nm in diameter) were present. Some of the CPON‐immunoreactive nerve terminals were found in close apposition to the pinealocyte cell membrane, making a synapticlike contact with the pinealocytes. Conclusions Our results show the presence of dense CPON‐IR innervation in the pig pineal gland. The ultrastructural localization of CPON‐IR nerve terminals shows that the peptide can be released to both perivascular and intercellular spaces. The functional role for this peptide in pig pineal gland is still an open question. Anat. Rec. 248:576‐582, 1997. © 1997 Wiley‐Liss, Inc.

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Section: Tion; Pigmentioning

confidence: 99%

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Przybylska‐Gornowicz¹,

Lewczuk²,

Møller

1997

Anat. Rec.

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“…Ultrastructural changes have been described in the pineal gland of rats [Satodate et al, 1980;Karasek et al, 1982], moles [Pevet and Smith, 1975], guinea pigs [Vollrath and Schmidt, 1969], and sows [Wyrzykowski et al, 1987]. Likewise, pineal activity has been related to pregnancy by biochemical studies performed in rats [Tigchelaar and Nalbandov, 1975] and humans [Pang et al, 1987].…”

Section: Introductionmentioning

confidence: 99%

Interstitial Cells in the Pineal Gland of Pregnant and Nonpregnant Viscachas <b><i>(Lagostomus maximus maximus)</i></b>: A Morphometric and Biochemical Study

Busolini

Gallol

Rodríguez

et al. 2018

Cells Tissues Organs

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The pineal gland of mammals undergoes morphological and biochemical changes throughout the gestation period. In viscachas, a seasonal breeding rodent, pregnancy lasts approximately 154 days and 3 stages can be defined, i.e., early, mid, and late pregnancy. The purpose of this study is to analyze morphometric variations in the expression of S-100 protein, glial fibrillary acidic protein (GFAP), and vimentin in the interstitial cells (IC) in pregnant and nonpregnant viscachas by immunohistochemistry (IHC). We also aim to evaluate a probable relation between glandular activity and pregnancy. The immunopositive percentage area (%IA) for the studied proteins and the number of immunoreactive cells against the S-100 protein with a visible nucleus (nº IC-S-100) were analyzed. Estradiol and progesterone serum levels were also determined by RIA. Variations in the expression of the S-100 protein and GFAP, as well as changes in the nº IC-S-100 related to serum hormone levels, were found between pregnant and nonpregnant viscachas. Viscachas in mid pregnancy exhibited the highest values of %IA for the analyzed proteins, followed by females in late and early pregnancy, while the nonpregnant ones showed the lowest values for all of the groups studied. Likewise, the nº IC-S-100 also varied following the same pattern. Thus, these variations seem to indicate a direct relationship between glandular activity and gonadal hormone levels. On these grounds, we may conclude that IC undergo changes in relation to ovarian hormone levels and participate in the regulation of glandular activity during pregnancy. However, further research is necessary to elucidate this relationship.

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“…The pineal gland of the domestic pig, which is the subject of the present study, shows several species-specific morphological features (Wyrzykowski et al 1987, Lewczuk et al 1994. Pinealocytes in the pig distinguish from these cells in other mammalian species by the presence of numerous cytoplasmic dense bodies called MBB (Wyrzykowski et al 1987).…”

Section: Introductionmentioning

confidence: 99%

“…The morphology and relative volume of MBB change in different physiological stages (Wyrzykowski et al 1987, Przybylska et al 1990) and under experimental conditions Przybylska-Gornowicz 1997a, 2000b). Also, the specificity of pig pineal physiology is well documented Przybylska-Gornowicz 1997b, 2000a).…”

Section: Introductionmentioning

confidence: 99%

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Neuropeptide Y as a presynaptic modulator of norepinephrine release from the sympathetic nerve fibers in the pig pineal gland

Ziółkowska¹,

Lewczuk²,

Przybylska‐Gornowicz³

2015

Polish Journal of Veterinary Sciences

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Norepinephrine (NE) released from the sympathetic nerve endings is the main neurotransmitter controlling melatonin synthesis in the mammalian pineal gland. Although neuropeptide Y (NPY) co-exists with NE in the pineal sympathetic nerve fibers it also occurs in a population of non-adrenergic nerve fibers located in this gland. The role of NPY in pineal physiology is still enigmatic. The present study characterizes the effect of NPY on the depolarization-evoked 3 H-NE release from the pig pineal explants.The explants of the pig pineal gland were loaded with 3 H-NE in the presence of pargyline and superfused with Tyrode medium. They were exposed twice to the modified Tyrode medium containing 60 mM of K + to evoke the 3 H-NE release via depolarization. NPY, specific agonists of Y 1 -and Y 2 -receptors and pharmacologically active ligands of α 2 -adrenoceptors were added to the medium before and during the second depolarization. The radioactivity was measured in medium fractions collected every 2 minutes during the superfusion. NPY (0.1 -10 μM) significantly decreased the depolarization-induced 3 H-NE release. Similar effect was observed after the treatment with Y 2 -agonist: NPY 13-36 , but not with Y 1 -agonist: [Leu 31 ,Pro 34 ]-NPY. The tritium overflow was lower in the explants exposed to the 5 μM NPY and 1 μM rauwolscine than to rauwolscine only. The effects of 5 μM NPY and 0.05 μM UK 14,304 on the depolarization-evoked 3 H-NE release were additive.The results show that NPY is involved in the regulation of NE release from the sympathetic terminals in the pig pineal gland, inhibiting this process via Y 2 -receptors.

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Morphology of the Pineal Glin Pregnant Nursing Sows

Cited by 10 publications

References 19 publications

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Demonstration of nerve fibers containing the C-terminal flanking peptide of neuropeptide Y (CPON) in the pig pineal gland (Sus domesticus): An immunocytochemical study by light and electron microscopy

Interstitial Cells in the Pineal Gland of Pregnant and Nonpregnant Viscachas <b><i>(Lagostomus maximus maximus)</i></b>: A Morphometric and Biochemical Study

Neuropeptide Y as a presynaptic modulator of norepinephrine release from the sympathetic nerve fibers in the pig pineal gland

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