Morphology of the Nucleoprotein Component of Rabies Virus

Hummeler, Klaus; Tomassini, N; Sokol, F.; Kuwert, E.; Koprowski, Hilary

doi:10.1128/jvi.2.10.1191-1199.1968

Cited by 52 publications

(16 citation statements)

References 28 publications

(24 reference statements)

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“…This description strongly suggests that rabies virus RNA synthesis is almost inactive in the inclusion bodies. Rabiesinduced inclusion bodies have been thought to be composed of accumulated viral nucleocapsids (5). Considering this assumption and our observations together, it is suggested that the accumulation of L and P proteins into the inclusion bodies would be responsible in part for the apparently lower rate of viral RNA synthesis than that seen in the VSV-infected cells in which little accumulation of viral nucleocapsids could be seen.…”

Section: Studies On the Behavior Of L Protein In The Cellsupporting

confidence: 51%

Studies on Rabies Virus RNA Polymerase: 1. cDNA Cloning of the Catalytic Subunit (L Protein) of Avirulent HEP‐Flury Strain and Its Expression in Animal Cells

Morimoto

Akamine

Takamatsu

et al. 1998

Microbiology and Immunology

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To investigate the RNA polymerase of rabies virus, we cloned a cDNA of the catalytic subunit (called L protein because of its large molecular size) of the HEP-Flury strain, an avirulent strain obtained by high frequencies of serial embryonated hen egg passages. Nucleotide sequencing showed that the cDNA encodes a long polypeptide of 2,127 amino acids (Mr. 242,938). A comparison of the deduced amino acid sequence with that of other strains (PV and SAD B19) indicated that the sequence was highly conserved, except for several amino acid substitutions which were accumulated in some limited regions. A fragment of the cDNA was used for expression in Escherichia coli (E. coli) to prepare the L antigen for raising the antibodies in rabbits. Immunoprecipitation studies with the rabbit antiserum showed that the polypeptides produced in the L cDNA-transfected COS-7 cells displayed almost the same electrophoretic mobility as that of authentic L protein.Immunofluorescence studies indicated that both L and P (another subunit of RNA polymerase) proteins displayed colocalized distribution with the nucleocapsid antigen (N) in the cytoplasmic inclusion bodies, where envelope proteins (G and M) were absent. On the other hand, expression of the L protein alone did not cause inclusion body-like granular distribution, suggesting that the inclusion body-like accumulation depends on certain interaction(s) with other viral gene products, probably with the ribonucleoproteins comprising the inclusion bodies.

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Section: Studies On the Behavior Of L Protein In The Cellsupporting

confidence: 51%

Studies on Rabies Virus RNA Polymerase: 1. cDNA Cloning of the Catalytic Subunit (L Protein) of Avirulent HEP‐Flury Strain and Its Expression in Animal Cells

Morimoto

Akamine

Takamatsu

et al. 1998

Microbiology and Immunology

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“…Such material consists of randomly orientated viral nucleocapsids. as described by Hummeler et al in 1968 [ 71 using ferritin-labelled anti-rabies antibodies. Tubular or bullet-shaped viral particles are also seen budding from the matrix into cisterns of endoplasmic reticulum (Figure 4 f, g) [12,151.…”

Section: Ultrastructure Of the Negri Bodiesmentioning

confidence: 99%

Rabies: interactions between neurons and viruses. A review of the history of Negri inclusion bodies

Kristensson¹,

Dk²,

Dk³

et al. 1996

Neuropathology Appl Neurobio

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The first clear-cut description of a virus-nerve cell interaction was made by Adelchi Negri in 1903 with the detection of cytoplasmic bodies (Negri bodies) in subsets of neurons in the brain from rabies-infected animals. A biographical sketch of Negri is given here; he was born in Perugia, Italy, in 1875 and died in Pavia in 1912. In 1900 Negri became assistant to Camillo Golgi, who encouraged him to study rabies-infected brains with histological techniques. The report of intraneuronal bodies described by Negri as specific for rabies stimulated an intense debate both concerning their diagnostic value and their nature. The diagnostic value was finally determined in a study by Negri's wife, Lina Negri-Luzzani, in 1913, while the viral nature of the bodies had to await the introduction of electron microscopy and immunohistochemistry. However, the true significance of the Negri bodies is still mysterious, since they only develop in subsets of infected neurons and occur mainly after infection with wild, so-called 'street', virus strains and not after infection with strains passaged in the laboratory, so-called 'fixed' strains.

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“…Rabies as a major zoonotic disease has a considerable public health, veterinary and economic impact. It is an acute fatal viral encephalomyelitis disease, caused by a filterable virus that belongs to family Rhabdoviridae group (Hummeler et al, 1968). Transmission of rabies occurs by bits of infected carnivores (Williams and Barker, 2001).…”

mentioning

confidence: 99%

Preparation of an inactivated penta cell culture vaccine against canine distemper, canine parvo, canine hepatitis "canine adeno-1", canine adeno-2 and rabies viruses for dogs

Aly¹,

Salama²

2005

Journal of Veterinary Medical Research

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A penta-dog inactivated cell culture vaccine was prepared to protect dogs against canine distemper virus, canine parvovirus, canine adenovirus1, 2 and rabies virus. The potency of this vaccine was compared with that of single inactivated vaccines prepared against each disease, in different groups of susceptible dogs. It was found that the protective dose of penta-dog vaccine (2ml) including the protective amounts of the five viral proteins resulted in full protection of vaccinated dogs against the challenge with virulent strain of the used viruses showing no antagonizing effect between each other with and no adverse postvaccinal reaction. So, the prepared inactivated cell culture penta-dog vaccine is a safe and potent vaccine for dogs which resulted in saving time, cost, and effort stress factors on animals and providing good immune statues.

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Morphology of the Nucleoprotein Component of Rabies Virus

Cited by 52 publications

References 28 publications

Studies on Rabies Virus RNA Polymerase: 1. cDNA Cloning of the Catalytic Subunit (L Protein) of Avirulent HEP‐Flury Strain and Its Expression in Animal Cells

Studies on Rabies Virus RNA Polymerase: 1. cDNA Cloning of the Catalytic Subunit (L Protein) of Avirulent HEP‐Flury Strain and Its Expression in Animal Cells

Rabies: interactions between neurons and viruses. A review of the history of Negri inclusion bodies

Preparation of an inactivated penta cell culture vaccine against canine distemper, canine parvo, canine hepatitis "canine adeno-1", canine adeno-2 and rabies viruses for dogs

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