“…They were distributed within the stratified squamous epithelium, which showed the same morphological features as those of epidermal Langerhans cells. The present TEM study also confirmed that the esophageal Langerhans cells occurred within the epithelium [8]. On the other hand, immunohistochemical studies already illustrated that the human epidermal Langerhans cells were more positive for anti-S-100 protein, anti-MHC-Class II protein, anti-CD54, anti-CD80, anti-CD 86, and anti-CD40 [2,4].…”
Section: Discussionsupporting
confidence: 84%
“…Langerhans cells in the human esophagus were also examined by electron microscopy [8]. They were distributed within the stratified squamous epithelium, which showed the same morphological features as those of epidermal Langerhans cells.…”
Section: Discussionmentioning
confidence: 99%
“…On the other hand, transmission electron microscopic observation of the human esophagus demonstrated that Langerhans cells were also present within the stratified squamous epithelium [4]. The ultrastructure of Langerhans cells in the human esophagus was clearly identical with that of the human skin [8]. Langerhans cells were characterized by the presence of lobed nuclei, cytoplasmic processes and Birbeck granules.…”
Our previous report illustrated that the epithelium of the human esophagus has numerous lymphocytes and Langerhans cells with Birbeck granules. In the present study, the distribution of Langerhans cells in the human esophagus was examined immunohistochemically. The human esophageal specimens were obtained by the surgery for esophageal carcinoma with informed consent. The tissue blocks were fixed with 10% formalin and embedded in paraffin. The thin sections were treated with anti-S-100 protein antibody, placed in IgG-gold (5 nm) solution as a second antibody, and then sensitized with the physical development method. The Langerhans cells, which showed a positive immunoreaction for the S-100, were distributed within the epithelium throughout the esophagus. Their distribution patterns differed according to the region of the esophagus. The Langerhans cells tended to increase in number as they moved further from the oral cavity toward the abdominal part. In this study, it was suggested that they played important roles in the immune response of the human esophagus.
“…They were distributed within the stratified squamous epithelium, which showed the same morphological features as those of epidermal Langerhans cells. The present TEM study also confirmed that the esophageal Langerhans cells occurred within the epithelium [8]. On the other hand, immunohistochemical studies already illustrated that the human epidermal Langerhans cells were more positive for anti-S-100 protein, anti-MHC-Class II protein, anti-CD54, anti-CD80, anti-CD 86, and anti-CD40 [2,4].…”
Section: Discussionsupporting
confidence: 84%
“…Langerhans cells in the human esophagus were also examined by electron microscopy [8]. They were distributed within the stratified squamous epithelium, which showed the same morphological features as those of epidermal Langerhans cells.…”
Section: Discussionmentioning
confidence: 99%
“…On the other hand, transmission electron microscopic observation of the human esophagus demonstrated that Langerhans cells were also present within the stratified squamous epithelium [4]. The ultrastructure of Langerhans cells in the human esophagus was clearly identical with that of the human skin [8]. Langerhans cells were characterized by the presence of lobed nuclei, cytoplasmic processes and Birbeck granules.…”
Our previous report illustrated that the epithelium of the human esophagus has numerous lymphocytes and Langerhans cells with Birbeck granules. In the present study, the distribution of Langerhans cells in the human esophagus was examined immunohistochemically. The human esophageal specimens were obtained by the surgery for esophageal carcinoma with informed consent. The tissue blocks were fixed with 10% formalin and embedded in paraffin. The thin sections were treated with anti-S-100 protein antibody, placed in IgG-gold (5 nm) solution as a second antibody, and then sensitized with the physical development method. The Langerhans cells, which showed a positive immunoreaction for the S-100, were distributed within the epithelium throughout the esophagus. Their distribution patterns differed according to the region of the esophagus. The Langerhans cells tended to increase in number as they moved further from the oral cavity toward the abdominal part. In this study, it was suggested that they played important roles in the immune response of the human esophagus.
“…Morphometric analysis was performed for quantification of CD20 + cells using the Metamorph Imaging system (Universal Imaging, West Chester, Pennsylvania, USA). Total stained cells and total surface area were quantified in the three compartments of the oesophageal mucosa: the epithelium, the vascular papillae (projections of the lamina propria towards the undersurface of the epithelium) 35 and the lamina propria, and results are expressed as number of positive cells per mm 2 of tissue. Tryptase + cells were counted in at least 10 non-overlapping fields and the results are expressed as the maximum number of positive cells per hpf.…”
Background:Eosinophilic oesophagitis (EO) is an emerging yet increasingly prevalent disorder characterised by a dense and selective eosinophilic infiltration of the oesophageal wall. While EO is considered an atopic disease primarily triggered by food antigens, disparities between standard allergen testing and clinical responses to exclusion diets suggest the participation of distinct antigen-specific immunoglobulin E (IgE) in the pathophysiology of EO.Aim:To find evidence for a local IgE response.Methods:Endoscopic biopsies of the distal oesophagus of atopic and non-atopic EO and control individuals (CTL) were processed for immunohistochemistry and immunofluorescence to assess the presence of B cells, mast cells, and IgE-bearing cells. Oesophageal RNA was analysed for the expression of genes involved in B cell activation, class switch recombination to IgE and IgE production, including germline transcripts (GLTs), activation-induced cytidine deaminase (AID), IgE heavy chain (Cε) and mature IgE mRNA using polymerase chain reaction and microarray analysis.Results:Regardless of atopy, EO showed increased density of B cells (p<0.05) and of IgE-bounded mast cells compared to CTL. Both EO and CTL expressed μGLT, εGLT, γ4GLT, AID, Cε and IgE mRNA. However, the frequency of expression of total GLTs (p = 0.002), εGLT (p = 0.024), and Cε (p = 0.0003) was significantly higher in EO than in CTL, independent of the atopic status.Conclusion:These results support the heretofore unproven occurrence of both local immunoglobulin class switching to IgE and IgE production in the oesophageal mucosa of EO patients. Sensitisation and activation of mast cells involving local IgE may therefore critically contribute to disease pathogenesis.
“…Accordingly, immune cells such as lymphocytes must be distributed within the stratified squamous epithelium to recognize antigens. It has been reported that lymphocytes and Langerhans cells are localized within the stratified squamous epithelium of the human oesophagus (Matsumoto et al., 1997). This study observed that TLR4 was strongly expressed within the stratified squamous epithelium, which can recognize antigens.…”
This study was performed to determine the localization strategies of Toll-like Receptor 4 (TLR4) in digestive tract (oesophagus, bulbodium, foregut, midgut and hindgut) of Blunt snout bream (Megalobrama amblycephala) using immunohistochemical staining method. TLR4 positive cells were observed throughout the digestive tract. In the oesophagus, some positive reactions in lamina propria were found around small blood vessels and there were also some positive cells within the stratified squamous epithelium. Lots of positive cells were observed in the muscular layer of the oesophagus. In bulbodium, foregut and hindgut, the expression of TLR4 was mainly restricted to the apical surface of epithelial cells located at the bottom of the mucosal folds and the mesenchymal cells in lamina propria. It was very interesting that epithelial cells in the midgut, but none in other parts, had many TLR4 positive cytoplasmic granular structures which were also periodic acid Schiff positive. These findings suggested that TLR4 was expressed in a compartmentalized manner in the Blunt snout bream (M. amblycephala) digestive tract and provided novel information about the in vivo localization of pattern recognition receptors.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.